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Proteomic 2DE Database for Spot Selection, Automated Annotation, and Data Analysis

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Lars Malmström,^† Johan Malmström,^† György Marko-Varga,*^‡ and Gunilla Westergren-Thorsson^†

Cell & Molecular Biology, University of Lund, BMC, C13, 221 84 Lund, Sweden, and Analytical Chemistry, University of Lund, Box 124, 221 00 Lund, Sweden

Journal of Proteome Research, 2002, 1 (2), pp 135–138

DOI: 10.1021/pr010004i

Publication Date (Web): February 2, 2002

Section:

History, Education, and Documentation

Abstract

We present a software solution that enables faster and more accurate data analysis of 2DE/MALDI TOF MS data. The software supports data analysis through a number of automated data selection functions and advanced graphical tools. Once protein identities are determined using MALDI TOF MS, automated data retrieval from online databases provides biological information. The software, called 2DDB, reduces analysis time to a fraction without losing any quality compared to more manual data analysis. The database contains over 100 000 data entries, and selected parts can be reached at http://2ddb.org.

Keywords: spot selection • automated annotation • 2DE/MALDI TOF MS • 2DDB

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This article has been cited by 3 ACS Journal articles (3 most recent appear below).

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Evaluation of Shotgun Sequencing for Proteomic Analysis of Human Plasma Using HPLC Coupled with Either Ion Trap or Fourier Transform Mass Spectrometry
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  Journal of Proteome Research2003 2 (4), 383-393
  This paper reports on studies directed to the characterization of the proteome of human plasma by the shotgun sequencing approach, namely the use of HPLC coupled to mass spectrometry (MS). The report will present data from two laboratories that allows the ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links