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Article

Elevated HGF Levels in Sera from Breast Cancer Patients Detected Using a Protein Microarray ELISA

Ronald L. Woodbury, Susan M. Varnum, and Richard C. Zangar*

Battelle, Pacific Northwest National Laboratory, Molecular Biosciences Department, P7-56, P.O. Box 999, Richland, Washington 99352

Journal of Proteome Research, 2002, 1 (3), pp 233–237

DOI: 10.1021/pr025506q

Publication Date (Web): April 18, 2002

To whom correspondence should be addressed. Phone: (509) 376-8596. Fax: (509) 376-6767. E-mail: richard.zangar@pnl.gov.

Abstract

We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 μL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarray format provides the potential for high-throughput quantitation of multiple biomarkers in parallel, as demonstrated with a multiplex analysis of five biomarker proteins.

Keywords: microarray • ELISA • breast cancer • hepatocyte growth factor

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