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Article

Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins

Rupa S. Rao,^†^‡ Steven R. Visuri,^† Mary T. McBride,^† Joanna S. Albala,^† Dennis L. Matthews,^†^‡ and Matthew A. Coleman*^†

Lawrence Livermore National Laboratory, 7000 East Avenue, P.O. Box 808, Livermore, California 94550, and Department of Biomedical Engineering, University of California Davis, Davis, California 95616

Journal of Proteome Research, 2004, 3 (4), pp 736–742

DOI: 10.1021/pr034130t

Publication Date (Web): May 7, 2004

†

Lawrence Livermore National Laboratory.

‡

Department of Biomedical Engineering, University of California Davis.

To whom correspondence should be addressed. L-448, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551. Telephone: +1−925-423-7687. Fax: +1−925-424-3130. E-mail address: coleman16@llnl.gov.

Abstract

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

Keywords: microarray • multiplex assay • bead-based assay • Luminex • detection • immunoassay

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