Quantitative Proteomic Analysis of Drug-Induced Changes in Mycobacteria

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Abstract

A new approach for qualitative and quantitative proteomic analysis using capillary liquid chromatography and mass spectrometry to study the protein expression response in mycobacteria following isoniazid treatment is discussed. In keeping with known effects on the fatty acid synthase II pathway, proteins encoded by the kas operon (AcpM, KasA, KasB, Accd6) were significantly overexpressed, as were those involved in iron metabolism and cell division suggesting a complex interplay of metabolic events leading to cell death.

Keywords: isoniazid • tuberculosis • protein profiling • liquid chromatography • mass spectrometry

Citing Articles

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This article has been cited by 9 ACS Journal articles (5 most recent appear below).

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