Comparison of Hydroxylated Print Additives on Antibody Microarray Performance

Peng Wu and David W. Grainger*
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872
J. Proteome Res., 2006, 5 (11), pp 2956–2965
DOI: 10.1021/pr060217d
Publication Date (Web): October 19, 2006
Copyright © 2006 American Chemical Society
*

 To whom correspondence should be addressed at current address:  Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112-5820. Tel, +1 801 581 4532; e-mail, david.grainger@pharm.utah.edu.

Abstract

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Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 °C for capturing model analytes, anti-human IL-1β, IL-4, and TNFα, on these microarraying slides.

Keywords: microarray • antibody • surface chemistry • immobilization • assay • diagnostics • shelf life

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History

  • Published In Issue November 03, 2006
  • Received May 5, 2006

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