Prev. Article Next Article Table of Contents

Article

Comprehensive Proteomic Analysis of Human Cervical−Vaginal Fluid

Supporting Info

Surendra Dasari,^‡ Leonardo Pereira,^† Ashok P. Reddy, John-Edward A. Michaels, Xinfang Lu,^‡ Thomas Jacob, Archana Thomas, Matthew Rodland,^‡ Charles T. Roberts, Jr.,^‡ Michael G. Gravett,^§ and Srinivasa R. Nagalla*^‡

Departments of Pediatrics and Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon 97239, Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98101, and ProteoGenix, Inc., Portland, Oregon 97213

J. Proteome Res., 2007, 6 (4), pp 1258–1268

DOI: 10.1021/pr0605419

Publication Date (Web): March 21, 2007

‡

Department of Pediatrics, Oregon Health and Science University.

†

Department of Obstetrics and Gynecology, Oregon Health and Science University.

ProteoGenix, Inc.

University of Washington.

To whom correspondence should be addressed. E-mail, nagallas@ohsu.edu; tel, 503-494-1928; fax, 503-494-4821.

Abstract

Cervical−vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding of human parturition and pathologic conditions affecting pregnancy. In this study, we performed a comprehensive survey of the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry and gel-electrophoresis-based protein separation and identification. In total, 150 unique proteins were identified using multiple protein identification algorithms. Metabolism (32%) and immune response-related (22%) proteins are the major functional categories represented in the CVF proteome. A comparison of the CVF, serum, and amniotic fluid proteomes showed that 77 proteins are unique to CVF, while 56 and 17 CVF proteins also occur in serum and amniotic fluid, respectively. This data set provides a foundation for evaluation of these proteins as potential CVF biomarkers for noninvasive diagnosis of pregnancy-related disorders.

Keywords: vaginal fluid • pregnancy • proteome • prematurity

View: Full Text HTML | Hi-Res PDF