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An Immuno-PF2D-MS/MS Proteomic Approach for Bacterial Antigenic Characterization: To Bacillus and Beyond

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Virginie Ruelle,^†^‡^§ Nandini Falisse-Poirrier,^†^§ Benaissa ElMoualij,^† Danièle Zorzi,^† Olivier Pierard,^† Ernst Heinen,^† Edwin De Pauw,^‡ and Willy Zorzi*^†

Center of Research on Prion Proteins, University of Liège, B-4000 Liège, Belgium, and Mass Spectrometry Laboratory, University of Liège, B-4000 Liège, Belgium

J. Proteome Res., 2007, 6 (6), pp 2168–2175

DOI: 10.1021/pr060661g

Publication Date (Web): May 9, 2007

Section:

Biochemical Methods

Abstract

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.

Keywords: Bacillus subtilis • flagellin • in-gel digest • mass spectrometry • PF2D

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