Design of Recombinant Antibody Microarrays for Cell Surface Membrane Proteomics

Linda Dexlin†‡, Johan Ingvarsson†‡, Björn Frendéus§, Carl A. K. Borrebaeck†‡ and Christer Wingren*†‡
Deptartment of Immunotechnology, BMC D13, Lund University, SE-221 84 Lund, Sweden, CREATE Health, BMC D13, Lund University, SE-221 84 Lund, Sweden, and Bioinvent International AB, SE-223 70 Lund, Sweden
J. Proteome Res., 2008, 7 (01), pp 319–327
DOI: 10.1021/pr070257x
Publication Date (Web): November 30, 2007
Copyright © 2008 American Chemical Society
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Deptartment of Immunotechnology, BMC D13, Lund University.

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‡

CREATE Health, BMC D13, Lund University.

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§

Bioinvent International AB.

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* Corresponding author: Christer Wingren, Dept. of Immunotechnology, Lund University, BMC D 13, SE-221 84 Lund, Sweden. Phone: +46-46-222 4323 . E-mail: christer.wingren@immun.lth.se.

Abstract

Abstract Image

Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

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  • Published In Issue January 04, 2008
  • Article ASAPNovember 30, 2007
  • Received: May 4, 2007
    Accepted:  ,
    Revised:  ,

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