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Amphibian Skin Secretomics: Application of Parallel Quadrupole Time-of-Flight Mass Spectrometry and Peptide Precursor cDNA Cloning to Rapidly Characterize the Skin Secretory Peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a Novel Peptide Family, the Hyposins

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Alan Hunter Thompson,^† Anthony John Bjourson,^† David Francis Orr,^† Chris Shaw,^‡ and Stephen McClean*^†

Institute of Biomedical Sciences, University of Ulster, Coleraine, Co Londonderry BT52 1SA, UK, and School of Pharmacy, Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK

J. Proteome Res., 2007, 6 (9), pp 3604–3613

DOI: 10.1021/pr0702666

Publication Date (Web): August 14, 2007

Section:

Biochemical Methods

Abstract

This study reports the variety of peptides present in the skin secretory peptidome of Phyllomedusa hypochondrialis azurea. Peptide structures, along with post-translational modifications, were elucidated by QTOF MS/MS analysis, cDNA sequencing, or a combination of both. Twenty-two peptides, including 19 novel structures, were identified from six different structural classes, including tryptophyllins, dermorphins, and a novel group of peptides termed hyposins. The study demonstrates the power of this combined approach to mine the rich peptidome compliment of the amphibian defensive skin secretome.

Keywords: amphiban skin • secretome • host defense • hyposin • mass spectrometry • de novo sequencing • Phyllomedusa hypochondrialis azurea

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This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Experimental Approach for Deep Proteome Measurements from Small-Scale Microbial Biomass Samples
Melissa R. Thompson, Karuna Chourey, Jennifer M. Froelich, Brian K. Erickson, Nathan C. VerBerkmoes and Robert L. Hettich
Analytical Chemistry2008 80 (24), 9517-9525
- Experimental Approach for Deep Proteome Measurements from Small-Scale Microbial Biomass Samples
  Melissa R. Thompson, Karuna Chourey, Jennifer M. Froelich, Brian K. Erickson, Nathan C. VerBerkmoes and Robert L. Hettich
  Analytical Chemistry2008 80 (24), 9517-9525
  Many methods of microbial proteome characterizations require large quantities of cellular biomass (>1−2 g) for sample preparation and protein identification. Our experimental approach differs from traditional techniques by providing the ability to ...
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