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Article

Experimental Approach for Assessing the Outcome Accuracy of Antibody Microarray Experiments

Supporting Info

Qiang Gu,* Thamil Mani Sivanandam, and Jessica Haymore

Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157

J. Proteome Res., 2007, 6 (11), pp 4210–4217

DOI: 10.1021/pr0703932

Publication Date (Web): October 2, 2007

To whom correspondence should be addressed. Dr. Qiang Gu, Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157; Phone, (336) 716−9226; Fax, (336) 716−4534; E-mail, qgu@wfubmc.edu.

Abstract

An experimental strategy for quality control of antibody microarray analyses is proposed. The method utilizes proteins that are prepared for regular antibody microarray experiments. There is no need to use exogenous positive or negative reference markers and no need to determine the absolute concentration of each individual protein in the sample. Validation experiments support the basic principle of the proposed approach. This method can be a useful tool for assessing the outcome accuracy of microarray experiments.

Keywords: microarrays • proteomics analysis • accuracy assessment • quality control

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