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Proteomic Analysis of Amniotic Fluid in Pregnancies with Turner Syndrome Fetuses

Ariadni Mavrou^†, Athanasios K. Anagnostopoulos^†^‡, Aggeliki Kolialexi^†, Konstantinos Vougas^‡, Nikos Papantoniou^§, Aris Antsaklis^§, Michael Fountoulakis^‡ and George Th. Tsangaris*^‡

Medical Genetics, Athens University School of Medicine, Athens, Greece, Proteomics Research Unit, Centre of Basic Research II, Biomedical Research Foundation, Academy of Athens, Athens, Greece, and 1st Department of Obstetrics & Gynaecology, Athens University School of Medicine, Athens, Greece

J. Proteome Res., 2008, 7 (5), pp 1862–1866

DOI: 10.1021/pr700588u

Publication Date (Web): March 26, 2008

†

Medical Genetics, Athens University School of Medicine.

‡

Biomedical Research Foundation.

1st Department of Obstetrics & Gynaecology, Athens University School of Medicine.

* Corresponding author: George Th. Tsangaris Ph.D., Proteomics Research Unit, Centre of Basic Research II, Biomedical Research Foundation, Academy of Athens, Soranou Efesiou 4, 115 27 Athens, Greece. Tel.: ++ 210 6597075 . Fax: ++ 210 6597545. E-mail: gthtsangaris@bioacademy.gr.

Abstract

Turner syndrome, occurring in 1:2500 female births, is caused by the complete or partial absence of one X chromosome. Amniotic fluid supernatant proteins from five second trimester pregnancies with Turner syndrome fetuses and five normal ones were analyzed by 2DE, MALDI-TOF-MS, and Western blot. Serotransferin, lumican, plasma retinol-binding protein, and apolipoprotein A-I were increased in Turner syndrome, while kininogen, prothrombin, and apolipoprotein A-IV were decreased. Since differentially expressed proteins are likely to cross the placenta barrier and be detected in maternal plasma, proteomic analysis may enhance research for noninvasive prenatal diagnosis of Turner syndrome.

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