Targeting Therapy for Breast Carcinoma by ATP Synthase Inhibitor Aurovertin B

Tsui-Chin Huang†, Hsin-Yi Chang†, Chun-Hua Hsu‡, Wen-Hung Kuo§, King-Jen Chang§ and Hsueh-Fen Juan*†#
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan, Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, Department of Surgery, National Taiwan University Hospital and College of Medicine, Taipei 100, Taiwan, Department of Life Science, National Taiwan University, Taipei 106, Taiwan, Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 106, Taiwan, and Center for Systems Biology and Bioinformatics, National Taiwan University, Taipei 106, Taiwan
J. Proteome Res., 2008, 7 (4), pp 1433–1444
DOI: 10.1021/pr700742h
Publication Date (Web): February 15, 2008
Copyright © 2008 American Chemical Society
†

Institute of Molecular and Cellular Biology, National Taiwan University.

,

These authors contributed equally to this work.

,
‡

Department of Agricultural Chemistry, National Taiwan University.

,
§

Department of Surgery, National Taiwan University Hospital and College of Medicine.

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* Address correspondence to: Hsueh-Fen Juan, Ph.D., Department of Life Science, Institute of Molecular and Cellular Biology, National Taiwan University, No 1, Sec. 4, Roosevelt Road, Taipei, 106 Taiwan. Tel: +886-2-33664536 . Fax: +886-2-23673374. E-mail: yukijuan@ntu.edu.tw.
,

Department of Life Science, National Taiwan University.

,

Institute of Biomedical Electronics and Bioinformatics, National Taiwan University.

,
#

Center for Systems Biology and Bioinformatics, National Taiwan University.

This article is part of the "Mammary Gland and Breast Cancer Protromics" special issue.

Abstract

Abstract Image

Targeting of tumor tissues is one of the most powerful approaches to accelerate the efficiency of anticancer treatments. The investigation of effective targets, including proteins specifically and abundantly expressed in abnormal regions, has been one of the most important research topics in cancer therapy. In this study, we performed a proteomic analysis on human breast carcinoma tissues to investigate the tumor-specific protein expression in breast carcinoma. Our study showed that ATP synthase was up-regulated in tumor tissues and was present on the plasma membrane of breast cancer cells. Furthermore, we treated the breast cancer cells with ATP synthase inhibitors and examined the inhibitory efficiency. Aurovertin B, an ATP synthase inhibitor, has strong inhibition on the proliferation of several breast cancer cell lines, but little influence on the normal cell line MCF-10A. Aurovertin B inhibits proliferation of breast cancer cells by inducing apoptosis and arresting cell cycle at the G0/G1 phase. This study showed aurovertin B can be used as an antitumorigenic agent and may be exploited in cancer chemotherapy.

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  • Published In Issue April 04, 2008
  • Article ASAPFebruary 15, 2008
  • Received: November 14, 2007
    Accepted:  ,
    Revised:  ,

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