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Letter

Myotube Assembly on Nanofibrous and Micropatterned Polymers

Supporting Info

Ngan F. Huang,^†^‡ Shyam Patel,^†^‡ Rahul G. Thakar,^†^‡ Jun Wu,^§ Benjamin S. Hsiao,^§ Benjamin Chu,^§ Randall J. Lee,^† and Song Li*^†^‡

Joint Graduate Program in Bioengineering, University of California San Francisco/University of California Berkeley, California 94720-1762, Department of Bioengineering and Center for Tissue Bioengineering, University of California Berkeley, California 94720, Department of Chemistry, Stony Brook University, Stony Brook, New York 11794-3400, and Department of Medicine, University of California San Francisco, San Francisco, California 94143

Nano Lett., 2006, 6 (3), pp 537–542

DOI: 10.1021/nl060060o

Publication Date (Web): February 16, 2006

Abstract

Skeletal muscle consists of parallel bundles of myotubes formed by the fusion of myoblasts. We fabricated nanofibrous and micropatterned polymers as cell culture substrates to guide the morphogenesis of muscular tissue. The nanoscale and microscale topographic features regulate cell and cytoskeleton alignment, myotube assembly, myotube striation, and myoblast proliferation. This bottom-up approach from nanoscale to tissue level demonstrates the potential of nanofibrous polymers for engineering the assembly of cell and tissue structure.

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