In vitro
- Estrogen-receptor binding/transcriptional activation
- Androgen-receptor binding/transcriptional activation
- Steroidogenesis (to detect substances that interfere with the production of steroids
by inhibiting cytochrome P450 enzymes)
- Placental aromatase (alternative assay to detect substances that interfere with
aromatase, an enzyme that converts testosterone to estradiol)
In vivo
- Rodent 3-day uterotrophic (assay for estrogenicity)
- Rodent 20-day pubertal female (assay for thyroid and estrogenic effects based on
the age vaginal opening occurs in rats)
- Rodent 5-7-day Hershberger (assay for antiandrogens)
- Rodent 14-day intact adult male (to detect effects on male reproductive organs
that are sensitive to antiandrogens and substances that inhibit testosterone
production)
- Rodent 20-day thyroid/pubertal male (to detect androgens and antiandrogens)
- Frog metamorphosis (assay for thyroid and antithyroid effects based on tail
resorption rate in frogs)
- Fish gonadal recrudescence (to detect substances that effect maturation
in fish)
|
