| Figure 4. Developing an HPLC Method of a Pharmaceutical Preparation.
Shown are the sequential isocratic steps involving re-injections of the sample at successively lower acetonitrile content in the mobile phase of 100, 80, 65, and 40%. The analyte peak is resolved from extraneous components at 40% acetonitrile. Because the column is equilibrated in 2 min between mobile phase change, total method development time is 15 min. Column: 3 x3 C18 (33 x 4.6 mm i.d., 3 µm), mobile phase: acetonitrile: water, flow rate: 2.5 mL/min, detection: UV @ 254 nm. Reprinted with permission from Reference 1. |
