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Characterization of Electron Transfer Dissociation in the Orbitrap Velos HCD Cell

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    Research Article

    Characterization of Electron Transfer Dissociation in the Orbitrap Velos HCD Cell
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    • Christian K. Frese
      Christian K. Frese
      Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands
      Netherlands Proteomics Centre, 3584 CH, Utrecht, The Netherlands
      More by Christian K. Frese
    • Dirk Nolting
      Dirk Nolting
      Thermo Fisher Scientific, 28199, Bremen, Germany
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    • A. F. Maarten Altelaar
      A. F. Maarten Altelaar
      Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands
      Netherlands Proteomics Centre, 3584 CH, Utrecht, The Netherlands
      More by A. F. Maarten Altelaar
    • Jens Griep-Raming
      Jens Griep-Raming
      Thermo Fisher Scientific, 28199, Bremen, Germany
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    • Shabaz Mohammed
      Shabaz Mohammed
      Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands
      Netherlands Proteomics Centre, 3584 CH, Utrecht, The Netherlands
      Departments of Chemistry and Biochemistry, University of Oxford, Physical & Theoretical Chemistry Laboratory, South Parks Road, OX1 3QZ, Oxford, UK
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    • Albert J. R. Heck
      Albert J. R. Heck
      Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands
      Netherlands Proteomics Centre, 3584 CH, Utrecht, The Netherlands
      More by Albert J. R. Heck
    Other Access OptionsSupporting Information (1)

    Journal of The American Society for Mass Spectrometry

    Cite this: J. Am. Soc. Mass Spectrom. 2013, 24, 11, 1663–1670
    Click to copy citationCitation copied!
    https://doi.org/10.1007/s13361-013-0618-9
    Published April 20, 2013
    Copyright © 2013 © American Society for Mass Spectrometry 2013
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    Abstract

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    Electron transfer dissociation (ETD) is commonly employed in ion traps utilizing rf fields that facilitate efficient electron transfer reactions. Here, we explore performing ETD in the HCD collision cell on an Orbitrap Velos instrument by applying a static DC gradient axially to the rods. This gradient enables simultaneous three dimensional, charge sign independent, trapping of cations and anions, initiating electron transfer reactions in the center of the HCD cell where oppositely charged ions clouds overlap. Here, we evaluate this mode of operation for a number of tryptic peptide populations and the top-down sequence analysis of ubiquitin. Our preliminary data show that performing ETD in the HCD cell provides similar fragmentation as ion trap-ETD but requires further optimization to match performance of ion trap-ETD.

    Copyright © 2013 © American Society for Mass Spectrometry 2013

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    This article is cited by 5 publications.

    1. Robert L. Schrader, Thomas E. Walker, David H. Russell. Modified Ion Source for the Improved Collisional Activation of Protein Complexes. Journal of the American Society for Mass Spectrometry 2023, 34 (5) , 977-980. https://doi.org/10.1021/jasms.3c00071
    2. Nicholas M. Riley and Joshua J. Coon . The Role of Electron Transfer Dissociation in Modern Proteomics. Analytical Chemistry 2018, 90 (1) , 40-64. https://doi.org/10.1021/acs.analchem.7b04810
    3. Christopher M. Rose, Matthew J. P. Rush, Nicholas M. Riley, Anna E. Merrill, Nicholas W. Kwiecien, Dustin D. Holden, Christopher Mullen, Michael S. Westphall, Joshua J. Coon. A Calibration Routine for Efficient ETD in Large-Scale Proteomics. Journal of the American Society for Mass Spectrometry 2015, 26 (11) , 1848-1857. https://doi.org/10.1007/s13361-015-1183-1
    4. Joe R. Cannon, Dustin D. Holden, and Jennifer S. Brodbelt . Hybridizing Ultraviolet Photodissociation with Electron Transfer Dissociation for Intact Protein Characterization. Analytical Chemistry 2014, 86 (21) , 10970-10977. https://doi.org/10.1021/ac5036082
    5. Darren T. Gass, Ana V. Quintero, Jacob B. Hatvany, Elyssia S. Gallagher. Metal adduction in mass spectrometric analyses of carbohydrates and glycoconjugates. Mass Spectrometry Reviews 2024, 43 (4) , 615-659. https://doi.org/10.1002/mas.21801
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    Journal of The American Society for Mass Spectrometry

    Cite this: J. Am. Soc. Mass Spectrom. 2013, 24, 11, 1663–1670
    Click to copy citationCitation copied!
    https://doi.org/10.1007/s13361-013-0618-9
    Published April 20, 2013
    Copyright © 2013 © American Society for Mass Spectrometry 2013
    Request reuse permissions

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