Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Am. Soc. Mass Spectrom. 2013, 24, 9, 1366-1375
Fragmentation of Neutral Amino Acids and Small Peptides by Intense, Femtosecond Laser Pulses
- Martin J. DuffyMartin J. DuffyCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Martin J. Duffy
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- Orla KellyOrla KellyCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Orla Kelly
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- Christopher R. CalvertChristopher R. CalvertCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Christopher R. Calvert
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- Raymond B. KingRaymond B. KingCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Raymond B. King
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- Louise BelshawLouise BelshawCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Louise Belshaw
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- Thomas J. KellyThomas J. KellyNational Centre for Plasma Science and Technology, School of Physical Sciences, Dublin City University, Dublin, Republic of IrelandMore by Thomas J. Kelly
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- John T. CostelloJohn T. CostelloNational Centre for Plasma Science and Technology, School of Physical Sciences, Dublin City University, Dublin, Republic of IrelandMore by John T. Costello
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- David J. TimsonDavid J. TimsonSchool of Biological Sciences, Queen’s University Belfast, BT9 7BL, Belfast, UKMore by David J. Timson
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- William A. BryanWilliam A. BryanDepartment of Physics, Swansea University, SA2 8PP, Swansea, UKMore by William A. Bryan
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- Thomas KierspelThomas KierspelSTFC Rutherford Appleton Laboratory, OX11 0QX, Didcot, Oxon, UKCenter for Free‐Electron Laser Science, DESY, Notkestrasse 85, 22607, Hamburg, GermanyMore by Thomas Kierspel
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- I. C. Edmond TurcuI. C. Edmond TurcuSTFC Rutherford Appleton Laboratory, OX11 0QX, Didcot, Oxon, UKMore by I. C. Edmond Turcu
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- Cephise M. CachoCephise M. CachoSTFC Rutherford Appleton Laboratory, OX11 0QX, Didcot, Oxon, UKMore by Cephise M. Cacho
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- Emma Springate
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- Ian D. WilliamsIan D. WilliamsCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Ian D. Williams
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- Jason B. GreenwoodJason B. GreenwoodCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, BT7 1NN, Belfast, UKMore by Jason B. Greenwood
Abstract
High power femtosecond laser pulses have unique properties that could lead to their application as ionization or activation sources in mass spectrometry. By concentrating many photons into pulse lengths approaching the timescales associated with atomic motion, very strong electric field strengths are generated, which can efficiently ionize and fragment molecules without the need for resonant absorption. However, the complex interaction between these pulses and biomolecular species is not well understood. To address this issue, we have studied the interaction of intense, femtosecond pulses with a number of amino acids and small peptides. Unlike previous studies, we have used neutral forms of these molecular targets, which allowed us to investigate dissociation of radical cations without the spectra being complicated by the action of mobile protons. We found fragmentation was dominated by fast, radical-initiated dissociation close to the charge site generated by the initial ionization or from subsequent ultrafast migration of this charge. Fragments with lower yields, which are useful for structural determinations, were also observed and attributed to radical migration caused by hydrogen atom transfer within the molecule.
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