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Comprehensive Lipidome Analysis by Shotgun Lipidomics on a Hybrid Quadrupole-Orbitrap-Linear Ion Trap Mass Spectrometer

  • Reinaldo Almeida
    Reinaldo Almeida
    Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences University of Southern Denmark, 5230, Odense, Denmark
  • Josch Konstantin Pauling
    Josch Konstantin Pauling
    Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences University of Southern Denmark, 5230, Odense, Denmark
  • Elena Sokol
    Elena Sokol
    Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences University of Southern Denmark, 5230, Odense, Denmark
    More by Elena Sokol
  • Hans Kristian Hannibal-Bach
    Hans Kristian Hannibal-Bach
    Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences University of Southern Denmark, 5230, Odense, Denmark
  • , and 
  • Christer S. Ejsing
    Christer S. Ejsing
    Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences University of Southern Denmark, 5230, Odense, Denmark
Cite this: J. Am. Soc. Mass Spectrom. 2015, 26, 1, 133–148
Publication Date (Web):November 13, 2014
Copyright © 2014 © American Society for Mass Spectrometry 2014

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    Abstract Image

    Here we report on the application of a novel shotgun lipidomics platform featuring an Orbitrap Fusion mass spectrometer equipped with an automated nanoelectrospray ion source. To assess the performance of the platform for in-depth lipidome analysis, we evaluated various instrument parameters, including its high resolution power unsurpassed by any other contemporary Orbitrap instrumentation, its dynamic quantification range and its efficacy for in-depth structural characterization of molecular lipid species by quadrupole-based higher-energy collisional dissociation (HCD), and ion trap-based resonant-excitation collision-induced dissociation (CID). This evaluation demonstrated that FTMS analysis with a resolution setting of 450,000 allows distinguishing isotopes from different lipid species and features a linear dynamic quantification range of at least four orders of magnitude. Evaluation of fragmentation analysis demonstrated that combined use of HCD and CID yields complementary fragment ions of molecular lipid species. To support global lipidome analysis, we designed a method, termed MSALL, featuring high resolution FTMS analysis for lipid quantification, and FTMS2 analysis using both HCD and CID and ITMS3 analysis utilizing dual CID for in-depth structural characterization of molecular glycerophospholipid species. The performance of the MSALL method was benchmarked in a comparative analysis of mouse cerebellum and hippocampus. This analysis demonstrated extensive lipidome quantification covering 311 lipid species encompassing 20 lipid classes, and identification of 202 distinct molecular glycerophospholipid species when applying a novel high confidence filtering strategy. The work presented here validates the performance of the Orbitrap Fusion mass spectrometer for in-depth lipidome analysis.

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