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Application of Tandem Two-Dimensional Mass Spectrometry for Top-Down Deep Sequencing of Calmodulin

  • Federico Floris
    Federico Floris
    Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK
  • Lionel Chiron
    Lionel Chiron
    CASC4DE, 20 Avenue du Neuhof, 67100, Strasbourg, France
  • Alice M. Lynch
    Alice M. Lynch
    Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK
  • Mark P. Barrow
    Mark P. Barrow
    Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK
  • Marc-André Delsuc
    Marc-André Delsuc
    CASC4DE, 20 Avenue du Neuhof, 67100, Strasbourg, France
    Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut National de la Santé et de la Recherche, U596; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7104, Université de Strasbourg, 67404, Illkirch-Graffenstaden, France
  • , and 
  • Peter B. O’Connor
    Peter B. O’Connor
    Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK
Cite this: J. Am. Soc. Mass Spectrom. 2018, 29, 8, 1700–1705
Publication Date (Web):June 4, 2018
https://doi.org/10.1007/s13361-018-1978-y
Copyright © 2018 © The Author(s) 2018

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    Abstract

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    Two-dimensional mass spectrometry (2DMS) involves simultaneous acquisition of the fragmentation patterns of all the analytes in a mixture by correlating their precursor and fragment ions by modulating precursor ions systematically through a fragmentation zone. Tandem two-dimensional mass spectrometry (MS/2DMS) unites the ultra-high accuracy of Fourier transform ion cyclotron resonance (FT-ICR) MS/MS and the simultaneous data-independent fragmentation of 2DMS to achieve extensive inter-residue fragmentation of entire proteins. 2DMS was recently developed for top-down proteomics (TDP), and applied to the analysis of calmodulin (CaM), reporting a cleavage coverage of about ~23% using infrared multiphoton dissociation (IRMPD) as fragmentation technique. The goal of this work is to expand the utility of top-down protein analysis using MS/2DMS in order to extend the cleavage coverage in top-down proteomics further into the interior regions of the protein. In this case, using MS/2DMS, the cleavage coverage of CaM increased from ~23% to ~42%.

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    Cited By

    This article is cited by 11 publications.

    1. Hannah M. Britt, Tristan Cragnolini, Konstantinos Thalassinos. Integration of Mass Spectrometry Data for Structural Biology. Chemical Reviews 2022, 122 (8) , 7952-7986. https://doi.org/10.1021/acs.chemrev.1c00356
    2. Nicholas D. Schmitt, Joshua M. Berger, Jeremy B. Conway, Jeffrey N. Agar. Increasing Top-Down Mass Spectrometry Sequence Coverage by an Order of Magnitude through Optimized Internal Fragment Generation and Assignment. Analytical Chemistry 2021, 93 (16) , 6355-6362. https://doi.org/10.1021/acs.analchem.0c04670
    3. Luis A. Macias, Inês C. Santos, Jennifer S. Brodbelt. Ion Activation Methods for Peptides and Proteins. Analytical Chemistry 2020, 92 (1) , 227-251. https://doi.org/10.1021/acs.analchem.9b04859
    4. Maria A. van Agthoven, David P. A. Kilgour, Alice M. Lynch, Mark P. Barrow, Tomos E. Morgan, Christopher A. Wootton, Lionel Chiron, Marc-André Delsuc, Peter B. O’Connor. Phase relationships in two-dimensional mass spectrometry. Journal of the American Society for Mass Spectrometry 2019, 30 (12) , 2594-2607. https://doi.org/10.1007/s13361-019-02308-1
    5. Frederik Lermyte, Yury O. Tsybin, Peter B. O’Connor, Joseph A. Loo. Top or Middle? Up or Down? Toward a Standard Lexicon for Protein Top-Down and Allied Mass Spectrometry Approaches. Journal of the American Society for Mass Spectrometry 2019, 30 (7) , 1149-1157. https://doi.org/10.1007/s13361-019-02201-x
    6. Federico Floris, Lionel Chiron, Alice M. Lynch, Mark P. Barrow, Marc-André Delsuc, Peter B. O’Connor. Top-Down Deep Sequencing of Ubiquitin Using Two-Dimensional Mass Spectrometry. Analytical Chemistry 2018, 90 (12) , 7302-7309. https://doi.org/10.1021/acs.analchem.8b00500
    7. Yulin Qi, Pingqing Fu, Dietrich A. Volmer. Analysis of natural organic matter via fourier transform ion cyclotron resonance mass spectrometry: an overview of recent non‐petroleum applications. Mass Spectrometry Reviews 2022, 41 (5) , 647-661. https://doi.org/10.1002/mas.21634
    8. Maria Teresa Melfi, Basem Kanawati, Philippe Schmitt-Kopplin, Luigi Macchia, Diego Centonze, Donatella Nardiello. Investigation of fennel protein extracts by shot-gun Fourier transform ion cyclotron resonance mass spectrometry. Food Research International 2021, 139 , 109919. https://doi.org/10.1016/j.foodres.2020.109919
    9. Wei Jia, Lin Shi, Feng Zhang, James Chang, Xiaogang Chu. High-throughput mass spectrometry scheme for screening and quantification of flavonoids in antioxidant nutraceuticals. Journal of Chromatography A 2019, 1608 , 460408. https://doi.org/10.1016/j.chroma.2019.460408
    10. Maria A. van Agthoven, Yuko P. Y. Lam, Peter B. O’Connor, Christian Rolando, Marc-André Delsuc. Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry. European Biophysics Journal 2019, 48 (3) , 213-229. https://doi.org/10.1007/s00249-019-01348-5
    11. Federico Floris, Peter B. O'Connor. Fundamentals of two dimensional Fourier transform mass spectrometry. 2019, 187-232. https://doi.org/10.1016/B978-0-12-814013-0.00007-7

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