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Confirmatory analysis of ethylglucuronide in urine by liquid-chromatography/electrospray ionization/tandem mass spectrometry according to forensic guidelines

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Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Am. Soc. Mass Spectrom. 2004, 15, 2, 188-193
    Research Article

    Confirmatory analysis of ethylglucuronide in urine by liquid-chromatography/electrospray ionization/tandem mass spectrometry according to forensic guidelines
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    • Wolfgang Weinmann
      Wolfgang Weinmann
      Forensic Toxicology, University Hospital, Institute of Legal Medicine, Albertstrasse 9, D-79104, Freiburg, Germany
      More by Wolfgang Weinmann
    • Patrick Schaefer
      Patrick Schaefer
      Forensic Toxicology, University Hospital, Institute of Legal Medicine, Albertstrasse 9, D-79104, Freiburg, Germany
      More by Patrick Schaefer
    • Annette Thierauf
      Annette Thierauf
      Forensic Toxicology, University Hospital, Institute of Legal Medicine, Albertstrasse 9, D-79104, Freiburg, Germany
      More by Annette Thierauf
    • André Schreiber
      André Schreiber
      Applied Biosystems, Darmstadt, Germany
      More by André Schreiber
    • Friedrich Martin Wurst
      Friedrich Martin Wurst
      Psychiatric University Clinic, Basel, Switzerland
      More by Friedrich Martin Wurst
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    Journal of the American Society for Mass Spectrometry

    Cite this: J Am Soc Mass Spectrom 2004, 15, 2, 188–193
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    https://doi.org/10.1016/j.jasms.2003.10.010
    Published February 1, 2004
    Copyright © 2004 © American Society for Mass Spectrometry 2004
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    Abstract

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    β-D-ethylglucuronide (EtG) is a stable Phase II metabolite of ethanol which can be detected in urine samples several days after elimination of ethanol. It is a useful diagnostic parameter for monitoring abstinence of alcoholics in alcohol withdrawal treatment. For this purpose, determination in urine is mainly performed by LC-MS, LC-MS/MS, or by GC-MS. For the mass spectrometric identification and detection of controlled substances in more sensitive fields such as forensic toxicology, workplace drug testing, doping analysis, and veterinary organic residue control, official guidelines have been released requiring a chromatographic separation and a minimum of two mass spectrometric transitions of the analyte. However, for detection of EtG none of the published LC-MS/MS methods could fulfill the minimum requirements of any of these guidelines. Therefore, an existing LC-MS/MS method has been modified by monitoring further MS/MS transitions instead of only one (deprotonated molecule [M − H]−/product ions: m/z 75, 85, 113, and 159 optional) with the aim of withstanding administrative or court scrutiny in forensic or workplace drug testing cases. Full method validation has been performed in accordance to guidelines of the German Society of Toxicology and Forensic Chemistry (GTFCh) and requirements of ISO 17025. One application field in the United States is a workplace monitoring program to detect surreptitious alcohol use among recovering health professionals, who by contract had agreed on total abstinence after drug and alcohol withdrawal therapy.

    Copyright © 2004 © American Society for Mass Spectrometry 2004

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    This article is cited by 114 publications.

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    Cite this: J Am Soc Mass Spectrom 2004, 15, 2, 188–193
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