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Microfluidic Synthesis of Biodegradable Polyethylene-Glycol Microspheres for Controlled Delivery of Proteins and DNA Nanoparticles

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Department of Bioengineering, Stanford University 300 Pasteur Drive, Edwards R105, MC5341, Stanford, California 94305, United States
MSTP Program, School of Medicine, Stanford University 300 Pasteur Drive, Stanford, California 94305, United States
§ Department of Biological Sciences, San Jose State University One Washington Square, San Jose, California 95192, United States
Department of Orthopaedic Surgery, Stanford University, 300 Pasteur Drive, Stanford, California 94305, United States
Cite this: ACS Biomater. Sci. Eng. 2015, 1, 3, 157–165
Publication Date (Web):February 10, 2015
Copyright © 2015 American Chemical Society

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    Abstract Image

    Polymeric microspheres represent an injectable platform for controlling the release of a variety of biologics; microspheres may be combined in a modular fashion to achieve temporal release of two or more biomolecules. Microfluidics offers a versatile platform for synthesizing uniform polymeric microspheres harboring a variety of biologics under relatively mild conditions. Poly(ethylene glycol) (PEG) is a bioinert polymer that can be easily tailored to encapsulate and control the release of biologics. In this study, we report the microfluidic synthesis of biodegradable PEG-based microparticles for controlled release of growth factors or DNA nanoparticles. Simple changes in microfluidic design increased the rate of microparticle formation and controlled the size of the microspheres. Mesh size and degradation rate were controlled by varying the PEG polymer weight percent from 7.5 to 15% (w/v), thus tuning the release of growth factors and DNA nanoparticles, which retained their bioactivity in assays of cell proliferation and DNA transfection, respectively. This platform may provide a useful tool for synthesizing microspheres for use as injectable carriers to achieve coordinated growth-factor or DNA nanoparticle release in therapeutic applications.

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    The following file is available free of charge on the ACS Publications website at DOI: 10.1021/ab500051v.

    • bFGF encapsulation efficiency (Figure S1) and microsphere formation rate (Table S1) (PDF)

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