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    Programmable Delivery of DNA through a Nanopipet
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    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, U.K., and MRC Clinical Science Centre, Division of Medicine, Imperial College School of Medicine, London, W12 0NN, U.K.
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    Analytical Chemistry

    Cite this: Anal. Chem. 2002, 74, 6, 1380–1385
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    https://doi.org/10.1021/ac015674m
    Published February 8, 2002
    Copyright © 2002 American Chemical Society
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    Abstract

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    We report the pulsed delivery of single-stranded DNA molecules through a nanopipet. The conical geometry of the pipet leads to a localized electric field, since all of the potential drop occurs in the tip region. Pulsatile delivery of DNA molecules can be achieved in an experimentally simple way with high precision by controlling the applied voltage. Single-molecule detection and fluorescence correlation spectroscopy in the nanopipet enable us to determine the number of molecules delivered. Anomalous slow diffusion of the DNA molecules in the pipet has also been observed. This nanopumping technique may have potential applications in local drug delivery and nanofabrication of biomolecules on surfaces in aqueous environments.

    Copyright © 2002 American Chemical Society

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    †

     University of Cambridge.

    ‡

     Imperial College School of Medicine.

    *

     To whom correspondence should be addressed. Tel:  +44 1223 336481. Fax:  +44 1223 336362. E-mail:  [email protected].

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    This article is cited by 80 publications.

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    Analytical Chemistry

    Cite this: Anal. Chem. 2002, 74, 6, 1380–1385
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ac015674m
    Published February 8, 2002
    Copyright © 2002 American Chemical Society
    Request reuse permissions

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