Technical Updates to the Bacterial Method for Nitrate Isotopic AnalysesClick to copy article linkArticle link copied!
Abstract

The bacterial conversion of aqueous nitrate (NO3−) to nitrous oxide (N2O) for isotopic analysis has found widespread use since its introduction (Sigman, D. M.; Casciotti, K. L.; Andreani, M.; Galanter, M.; Böhlke, J. K. Anal. Chem.2001, 73, 4145−4153; Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905−4912). The bacterial strain Pseudomonas aureofaciens (ATTC no. 13985) was shown to convert NO3− to N2O while retaining both N and O isotopic signatures, and automation of the isotopic analysis of N2O greatly increased the throughput of the method (Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905−4912). Continued development of the denitrifier method has led to increased precision and throughput of NO3− isotopic analysis. Presented here are several recent procedural modifications and the demonstration of their effectiveness.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 218 publications.
- Dandan Liu, Xueying Li, Yue Zhang, Qi Qiao, Lu Bai. Using a Multi-isotope Approach and Isotope Mixing Models to Trace and Quantify Phosphorus Sources in the Tuojiang River, Southwest China. Environmental Science & Technology 2023, 57
(19)
, 7328-7335. https://doi.org/10.1021/acs.est.2c07216
- Xin Wang, Naomi S. Wells, Wei Xiao, Jessica L. Hamilton, Adele M. Jones, Richard N. Collins. Mackinawite (FeS) Chemodenitrification of Nitrate (NO3–) under Acidic to Neutral pH Conditions and Its Stable N and O Isotope Dynamics. ACS Earth and Space Chemistry 2022, 6
(12)
, 2801-2811. https://doi.org/10.1021/acsearthspacechem.2c00158
- Bo Wang, Shuai Zheng, Yan Huang, Ying Wang, Zhenyu Zhu, Ran Ma, Yu Zhao, Xijie Yin, Jing Su, Juan Xiong, Benli Zhang, Youping Zhou. Novel GC/Py/GC/IRMS-Based Method for Isotope Measurements of Nitrate and Nitrite. I: Converting Nitrate to Benzyl Nitrate for δ18O Analysis. Analytical Chemistry 2020, 92
(18)
, 12216-12225. https://doi.org/10.1021/acs.analchem.0c01403
- Stephen D. Sebestyen, Donald S. Ross, James B. Shanley, Emily M. Elliott, Carol Kendall, John L. Campbell, D. Bryan Dail, Ivan J. Fernandez, Christine L. Goodale, Gregory B. Lawrence, Gary M. Lovett, Patrick J. McHale, Myron J. Mitchell, Sarah J. Nelson, Michelle D. Shattuck, Trent R. Wickman, Rebecca T. Barnes, Joel T. Bostic, Anthony R. Buda, Douglas A. Burns, Keith N. Eshleman, Jacques C. Finlay, David M. Nelson, Nobuhito Ohte, Linda H. Pardo, Lucy A. Rose, Robert D. Sabo, Sherry L. Schiff, John Spoelstra, Karl W. J. Williard. Unprocessed Atmospheric Nitrate in Waters of the Northern Forest Region in the U.S. and Canada. Environmental Science & Technology 2019, 53
(7)
, 3620-3633. https://doi.org/10.1021/acs.est.9b01276
- Danielle S. Boshers, Julie Granger, Craig R. Tobias, John K. Böhlke, Richard L. Smith. Constraining the Oxygen Isotopic Composition of Nitrate Produced by Nitrification. Environmental Science & Technology 2019, 53
(3)
, 1206-1216. https://doi.org/10.1021/acs.est.8b03386
- Dallin P. Jensen, Carey Gazis. Isotope Investigation of Nitrate in Soils and Agricultural Drains of the Lower Yakima Valley, Washington. ACS Earth and Space Chemistry 2018, 2
(12)
, 1249-1255. https://doi.org/10.1021/acsearthspacechem.8b00086
- Zhongjie Yu and Emily M. Elliott . Novel Method for Nitrogen Isotopic Analysis of Soil-Emitted Nitric Oxide. Environmental Science & Technology 2017, 51
(11)
, 6268-6278. https://doi.org/10.1021/acs.est.7b00592
- David X. Soto, Geoff Koehler, and Keith A. Hobson . Combining Denitrifying Bacteria and Laser Spectroscopy for Isotopic Analyses (δ15N, δ18O) of Dissolved Nitrate. Analytical Chemistry 2015, 87
(14)
, 7000-7005. https://doi.org/10.1021/acs.analchem.5b01119
- Mengyang Zhou, Julie Granger, Cesar B. Rocha, Samantha A. Siedlecki, Benedetto Barone, Angelicque E. White. Nitrogen Biogeochemistry of Adjacent Mesoscale Eddies in the North Pacific Subtropical Gyre. Global Biogeochemical Cycles 2025, 39
(2)
https://doi.org/10.1029/2024GB008206
- Wentao Wang, Zhiming Yu, Xiuxian Song, Yongquan Yuan, Zaixing Wu, Lianbao Chi. Isotopic composition of nitrate in the Western Tropical Pacific Ocean: Biogeochemical signals and their transport. Progress in Oceanography 2025, 46 , 103431. https://doi.org/10.1016/j.pocean.2025.103431
- Hongbin Gao, Gang Wang, Yanru Fan, Junfeng Wu, Mengyang Yao, Xinfeng Zhu, Xiang Guo, Bei Long, Jie Zhao. Tracing groundwater nitrate sources in an intensive agricultural region integrated of a self-organizing map and end-member mixing model tool. Scientific Reports 2024, 14
(1)
https://doi.org/10.1038/s41598-024-67735-x
- Emmi Kurosawa, Joanne M. Oakes. What is on the menu? Botanical carnivory in carnivorous plants of New England (
USA
). Freshwater Biology 2024, 69
(12)
, 1787-1799. https://doi.org/10.1111/fwb.14341
- Yuanzhe Ni, Li Luo, Shuhan Liu, Jianbin Huang, Yuxiao Li, Jianhua Qi. Refined source apportionment of nitrate aerosols based on isotopes and emission inventories in coastal city of northern China. Science of The Total Environment 2024, 957 , 177388. https://doi.org/10.1016/j.scitotenv.2024.177388
- Barbara Bayer, Katharina Kitzinger, Nicola L. Paul, Justine B. Albers, Mak A. Saito, Michael Wagner, Craig A. Carlson, Alyson E. Santoro. Contribution of ammonia oxidizers to inorganic carbon fixation in the dark ocean. 2024https://doi.org/10.1101/2024.11.16.623942
- Siqi Wu, Xianhui Sean Wan, Moge Du, Xirong Chen, Corday R. Selden, Mar Benavides, Sophie Bonnet, Carolin R. Löscher, M. Robert Hamersley, Margaret R. Mulholland, Xiuli Yan, Shuh-Ji Kao. Heterogeneity of nitrogen fixation in the mesopelagic zone of the South China Sea. Frontiers in Marine Science 2024, 11 https://doi.org/10.3389/fmars.2024.1495649
- Zanfang Jin, Jiawen Li, Qiang Yang, Yasheng Shi, Xun Lin, Fan Chen, Qifang Chen, Zhili Chen, Feili Li. Nitrogen isotope characteristics and importance of NOx from biomass burning in China. Science of The Total Environment 2024, 951 , 175430. https://doi.org/10.1016/j.scitotenv.2024.175430
- Wei‐Guo Fan, Xue‐Yan Liu, Mingzhong Zhou, Wei Song, Yongyun Hu, Yanan Shen, Cong‐Qiang Liu. Sedimentary
17
O‐Nitrate Evidence for Phanerozoic Aridity and Humidity Oscillations in South China. Geophysical Research Letters 2024, 51
(20)
https://doi.org/10.1029/2024GL111475
- Akira Kurasawa, Yuji Onishi, Keisuke Koba, Keitaro Fukushima, Hiromi Uno. Sequential migrations of diverse fish community provide seasonally prolonged and stable nutrient inputs to a river. Science Advances 2024, 10
(43)
https://doi.org/10.1126/sciadv.adq0945
- Emmi Kurosawa, Naomi S Wells, Robert Gibson, Zachary Lyons, Richard Kesseli, Joanne M Oakes. To eat or not to eat: novel stable isotope models reveal a shift in carnivory with nutrient availability for aquatic
Utricularia
spp.. Annals of Botany 2024, 465 https://doi.org/10.1093/aob/mcae119
- Alexandra Auderset, François Fripiat, Roger C. Creel, Lukas Oesch, Anja S. Studer, Janne Repschläger, Ed Hathorne, Hubert Vonhof, Ralf Schiebel, Laura Gordon, Kira Lawrence, Haojia Abby Ren, Gerald H. Haug, Daniel M. Sigman, Alfredo Martínez‐García. Sea Level Modulation of Atlantic Nitrogen Fixation Over Glacial Cycles. Paleoceanography and Paleoclimatology 2024, 39
(8)
https://doi.org/10.1029/2024PA004878
- R. K. Thomas, S. E. Fawcett, H. J. Forrer, C. M. Robinson, A. N. Knapp. Estimates of the Isotope Effect for Nitrate Assimilation in the Indian Sector of the Southern Ocean. Journal of Geophysical Research: Oceans 2024, 129
(7)
https://doi.org/10.1029/2023JC020830
- Weilong Jiang, Fang Yang, Xihuan Wang, Qingfeng Miao, Meicheng Ji, Ting Pan, Qianqian Wang, Zhiming Han, Weiying Feng, Haiqing Liao. Improved determination of nitrate isotopes in irrigated agricultural areas and Bayesian mixing model quantification of nitrate sources and fate. Agricultural Water Management 2024, 298 , 108872. https://doi.org/10.1016/j.agwat.2024.108872
- Zelin Wang, Wangjia Ji, Furong Zhang, Yidi Liu, Zhi Li. Identifying the nitrate transport and transformations under apple orchards in the loess depositusing stable isotopes of water and nitrate. Agriculture, Ecosystems & Environment 2024, 364 , 108885. https://doi.org/10.1016/j.agee.2024.108885
- Momoka Suto, Hiroto Kawashima. Water-soluble ions and nitrogen and oxygen stable isotope ratios in nitrate in sake in Akita, Japan. LWT 2024, 198 , 115963. https://doi.org/10.1016/j.lwt.2024.115963
- Jinglong Mu, Shuai Ding, Su Mei Liu, Guodong Song, Xiaoyan Ning, Xiaotong Zhang, Wenqi Xu, Hongmei Zhang. Multiple isotopes decipher the nitrogen cycle in the cascade reservoirs and downstream in the middle and lower Yellow River: Insight for reservoir drainage period. Science of The Total Environment 2024, 918 , 170625. https://doi.org/10.1016/j.scitotenv.2024.170625
- Zanfang Jin, Jiawen Li, Yasheng Shi, Jing Hu, Yuchen Cai, Zhili Chen, Feili Li. Using PMF model and Bayesian model to quantify NOx sources in non-typhoons and Super Typhoon Lekima (2019) in Hangzhou. Atmospheric Environment 2024, 319 , 120282. https://doi.org/10.1016/j.atmosenv.2023.120282
- Xinwei Ren, Fu-Jun Yue, Jianhui Tang, Cai Li, Si-Liang Li. Nitrate transformation and source tracking of rivers draining into the Bohai Sea using a multi-tracer approach combined with an optimized Bayesian stable isotope mixing model. Journal of Hazardous Materials 2024, 463 , 132901. https://doi.org/10.1016/j.jhazmat.2023.132901
- Simone Moretti, Nicolas N. Duprey, Alan D. Foreman, Anthea Arns, Sven Brömme, Jonathan Jung, Xuyuan E. Ai, Alexandra Auderset, Aaron L. Bieler, Camino Eck, Jesse Farmer, Barbara Hinnenberg, Matthew Lacerra, Jennifer Leichliter, Tina Lüdecke, Sergey Oleynik, Florian Rubach, Mareike Schmitt, Marissa Vink, Tanja Wald, Maayan Yehudai, Daniel M. Sigman, Alfredo Martínez‐García. Analytical improvements and assessment of long‐term performance of the oxidation–denitrifier method. Rapid Communications in Mass Spectrometry 2024, 38
(1)
https://doi.org/10.1002/rcm.9650
- Nestor Arandia-Gorostidi, Alexander L Jaffe, Alma E Parada, Bennett J Kapili, Karen L Casciotti, Rebecca S R Salcedo, Chloé M J Baumas, Anne E Dekas. Urea assimilation and oxidation support activity of phylogenetically diverse microbial communities of the dark ocean. The ISME Journal 2024, 18
(1)
https://doi.org/10.1093/ismejo/wrae230
- Noémie Choisnard, Nicolas Noel Duprey, Tanja Wald, Martin Thibault, Fanny Houlbrèque, Alan D. Foreman, Pascale Cuet, Mireille M. M. Guillaume, Hubert Vonhof, Daniel M. Sigman, Gerald H. Haug, Jean‐François Maguer, Stéphane L'Helguen, Alfredo Martínez‐García, Anne Lorrain. Tracing the fate of seabird‐derived nitrogen in a coral reef using nitrate and coral skeleton nitrogen isotopes. Limnology and Oceanography 2024, 37 https://doi.org/10.1002/lno.12485
- Le Deng, Hong Liu, Yonghui Zhang, Weiwei Li, Yongzhi Chen. Advances in the research on N and O dual isotope analysis of biological nitrogen removal performance. Journal of Water Process Engineering 2024, 57 , 104595. https://doi.org/10.1016/j.jwpe.2023.104595
- Xuefeng Peng, David J. Yousavich, Annie Bourbonnais, Frank Wenzhöfer, Felix Janssen, Tina Treude, David L. Valentine. The fate of fixed nitrogen in Santa Barbara Basin sediments during seasonal anoxia. Biogeosciences 2024, 21
(12)
, 3041-3052. https://doi.org/10.5194/bg-21-3041-2024
- Colette L. Kelly, Nicole M. Travis, Pascale Anabelle Baya, Claudia Frey, Xin Sun, Bess B. Ward, Karen L. Casciotti. Isotopomer labeling and oxygen dependence of hybrid nitrous oxide production. Biogeosciences 2024, 21
(14)
, 3215-3238. https://doi.org/10.5194/bg-21-3215-2024
- Li Rongfu, Liao Wencheng, Liang Peiyu, Yao Zhong, Deng Mi, Liu Yajun, Liu Lizhen, Wu Yongming, . Driving factors of algal bloom and nitrogen sources tracing of a drinking water source reservoir in Lake Poyang Basin. Journal of Lake Sciences 2024, 36
(4)
, 1014-1024. https://doi.org/10.18307/2024.0412
- Josie L. Mottram, Anne M. Gothmann, Maria G. Prokopenko, Austin Cordova, Veronica Rollinson, Katie Dobkowski, Julie Granger. Quantifying the
δ
15
N trophic offset in a cold-water scleractinian coral (CWC): implications for the CWC diet and coral
δ
15
N as a marine N cycle proxy. Biogeosciences 2024, 21
(5)
, 1071-1091. https://doi.org/10.5194/bg-21-1071-2024
- Nicole M. Travis, Colette L. Kelly, Karen L. Casciotti. Testing the influence of light on nitrite cycling in the eastern tropical North Pacific. Biogeosciences 2024, 21
(8)
, 1985-2004. https://doi.org/10.5194/bg-21-1985-2024
- Yifan Hou, Shihui Wang, Yukun Ma, Zhenyao Shen, Ashantha Goonetilleke. Influence of landscape patterns on nitrate and particulate organic nitrogen inputs to urban stormwater runoff. Journal of Environmental Management 2023, 348 , 119190. https://doi.org/10.1016/j.jenvman.2023.119190
- Heather J. Forrer, Sophie Bonnet, Rachel K. Thomas, Olivier Grosso, Cecile Guieu, Angela N. Knapp. Quantifying N2 fixation and its contribution to export production near the Tonga-Kermadec Arc using nitrogen isotope budgets. Frontiers in Marine Science 2023, 10 https://doi.org/10.3389/fmars.2023.1249115
- Anya V. Hess, Alexandra Auderset, Yair Rosenthal, Kenneth G. Miller, Xiaoli Zhou, Daniel M. Sigman, Alfredo Martínez-García. A well-oxygenated eastern tropical Pacific during the warm Miocene. Nature 2023, 619
(7970)
, 521-525. https://doi.org/10.1038/s41586-023-06104-6
- Claudette Proctor, Pierre Coupel, Karen Casciotti, Jean‐Eric Tremblay, Emily Zakem, Kevin R. Arrigo, Matthew M. Mills. Light, ammonium,
pH
, and phytoplankton competition as environmental factors controlling nitrification. Limnology and Oceanography 2023, 68
(7)
, 1490-1503. https://doi.org/10.1002/lno.12359
- Ping Zhang, Xiao-Dan Wang, Fu-Jun Yue, Sai-Nan Chen, Zi-Han Liu, Meng Lu, Zhi-Yu Shi. Dynamic characteristics of nitrogen transport in various land use in a typical karst catchment during rainfall events. Environmental Earth Sciences 2023, 82
(13)
https://doi.org/10.1007/s12665-023-10980-6
- Meng Lu, Fu-Jun Yue, Xiao-Dan Wang, Zi-Han Liu, Zhi-Yu Shi, Ping Zhang. Identify nitrogen transport paths and sources contribution in karst valley depression area using isotopic approach. Journal of Environmental Management 2023, 337 , 117751. https://doi.org/10.1016/j.jenvman.2023.117751
- Emilee Severe, Isabella M. Errigo, Mary Proteau, Sayedeh Sara Sayedi, Tamara Kolbe, Jean Marçais, Zahra Thomas, Christophe Petton, François Rouault, Camille Vautier, Jean-Raynald de Dreuzy, Florentina Moatar, Luc Aquilina, Rachel L. Wood, Thierry LaBasque, Christophe Lécuyer, Gilles Pinay, Benjamin W. Abbott. Deep denitrification: Stream and groundwater biogeochemistry reveal contrasted but connected worlds above and below. Science of The Total Environment 2023, 880 , 163178. https://doi.org/10.1016/j.scitotenv.2023.163178
- Colette L. Kelly, Cara Manning, Claudia Frey, Jan Kaiser, Noah Gluschankoff, Karen L. Casciotti. Pyisotopomer: A Python package for obtaining intramolecular isotope ratio differences from mass spectrometric analysis of nitrous oxide isotopocules. Rapid Communications in Mass Spectrometry 2023, 37
(11)
https://doi.org/10.1002/rcm.9513
- Sophie Bonnet, Cécile Guieu, Vincent Taillandier, Cédric Boulart, Pascale Bouruet-Aubertot, Frédéric Gazeau, Carla Scalabrin, Matthieu Bressac, Angela N. Knapp, Yannis Cuypers, David González-Santana, Heather J. Forrer, Jean-Michel Grisoni, Olivier Grosso, Jérémie Habasque, Mercedes Jardin-Camps, Nathalie Leblond, Frédéric A. C. Le Moigne, Anne Lebourges-Dhaussy, Caroline Lory, Sandra Nunige, Elvira Pulido-Villena, Andrea L. Rizzo, Géraldine Sarthou, Chloé Tilliette. Natural iron fertilization by shallow hydrothermal sources fuels diazotroph blooms in the ocean. Science 2023, 380
(6647)
, 812-817. https://doi.org/10.1126/science.abq4654
- Leonard I. Wassenaar, Cedric Douence, Susan Fortson, Douglas S. Baer. Automated rapid triple-isotope (
δ
15
N,
δ
18
O,
δ
17
O) analyses of nitrate by Ti(III) reduction and N
2
O laser spectrometry. Isotopes in Environmental and Health Studies 2023, 59
(3)
, 297-308. https://doi.org/10.1080/10256016.2023.2222222
- Jing Hu, Mengyan Pan, Yuliang Li, Meng Xing, Yunning Cao, Kaili Yang, Weiguo Liu. Discussion on the need for correction during isotopic analysis of nitrogen by the denitrifier method. RSC Advances 2023, 13
(20)
, 13834-13839. https://doi.org/10.1039/D3RA00371J
- Zanfang Jin, Jiazheng Xiao, Hongyu Ye, Guowei Fu, Guangyao Li, Wangbin Wu, Feili Li. Determination of nitrogen sources and losses in surface runoff from different lands at a watershed scale. Environmental Science and Pollution Research 2023, 30
(22)
, 63052-63064. https://doi.org/10.1007/s11356-023-26459-7
- Zhong-Jun Wang, Fu-Jun Yue, Li-Li Xue, Yu-Chun Wang, Cai-Qing Qin, Jie Zeng, Hu Ding, Yu-Cong Fu, Si-Liang Li. Soil nitrogen transformation in different land use and implications for karst soil nitrogen loss controlling. CATENA 2023, 225 , 107026. https://doi.org/10.1016/j.catena.2023.107026
- Xin Wang, Naomi S. Wells, Wei Xiao, Jessica L. Hamilton, Adele M. Jones, Richard N. Collins. Abiotic reduction of nitrate to ammonium by iron (oxy)(hydr)oxides and its stable isotope (δ15N, δ18O) dynamics. Geochimica et Cosmochimica Acta 2023, 347 , 28-41. https://doi.org/10.1016/j.gca.2023.02.013
- Xin Sun, Claudia Frey, Bess B. Ward. Nitrite Oxidation Across the Full Oxygen Spectrum in the Ocean. Global Biogeochemical Cycles 2023, 37
(4)
https://doi.org/10.1029/2022GB007548
- Germain Esquivel-Hernández, Ioannis Matiatos, Ricardo Sánchez-Murillo, Yuliya Vystavna, Raffaella Balestrini, Naomi S. Wells, Lucilena R. Monteiro, Somporn Chantara, Wendell Walters, Leonard I. Wassenaar. Nitrate isotopes (
δ
15
N,
δ
18
O) in precipitation: best practices from an international coordinated research project. Isotopes in Environmental and Health Studies 2023, 59
(2)
, 127-141. https://doi.org/10.1080/10256016.2023.2177649
- Mengru Jia, Roland Bol, Annemieke Kooijman, Wim W. Wessel, Albert Tietema. A decision support tool for the selection of 15 N analysis methods of ammonium and nitrate. Nutrient Cycling in Agroecosystems 2023, 125
(2)
, 309-343. https://doi.org/10.1007/s10705-022-10227-z
- Wei Song, Xue-Yan Liu. Nitrogen isotope signatures of oxidized nitrogen species from biomass burning. Applied Geochemistry 2023, 150 , 105569. https://doi.org/10.1016/j.apgeochem.2023.105569
- Wei Song, Zhi-Li Chen, Yi-Meng Yin, Xue-Yan Liu. Primary nitrate from combustion-related sources biases the Δ17O differentiation of formation pathway contributions of atmospheric secondary nitrate. Atmospheric Environment 2023, 296 , 119574. https://doi.org/10.1016/j.atmosenv.2022.119574
- Alma E. Parada, Xavier Mayali, Peter K. Weber, Jessica Wollard, Alyson E. Santoro, Jed A. Fuhrman, Jennifer Pett‐Ridge, Anne E. Dekas. Constraining the composition and quantity of organic matter used by abundant marine Thaumarchaeota. Environmental Microbiology 2023, 25
(3)
, 689-704. https://doi.org/10.1111/1462-2920.16299
- Rebecca S. Robinson, Sandi M. Smart, Jonathan D. Cybulski, Kelton W. McMahon, Basia Marcks, Catherine Nowakowski. Insights from Fossil-Bound Nitrogen Isotopes in Diatoms, Foraminifera, and Corals. Annual Review of Marine Science 2023, 15
(1)
, 407-430. https://doi.org/10.1146/annurev-marine-032122-104001
- Jesse R. Farmer, Tamara Pico, Ona M. Underwood, Rebecca Cleveland Stout, Julie Granger, Thomas M. Cronin, François Fripiat, Alfredo Martínez-García, Gerald H. Haug, Daniel M. Sigman. The Bering Strait was flooded 10,000 years before the Last Glacial Maximum. Proceedings of the National Academy of Sciences 2023, 120
(1)
https://doi.org/10.1073/pnas.2206742119
- Wei Song, Xue-Yan Liu. Source oxygen contributions of primary nitrate emitted from biomass burning. Science of The Total Environment 2023, 854 , 158736. https://doi.org/10.1016/j.scitotenv.2022.158736
- Jie Zeng, Guilin Han, Shitong Zhang, Rui Qu. Nitrate dynamics and source identification of rainwater in Beijing during rainy season: Insight from dual isotopes and Bayesian model. Science of The Total Environment 2023, 856 , 159234. https://doi.org/10.1016/j.scitotenv.2022.159234
- Zeyu Sun, Zheng Zong, Yang Tan, Chongguo Tian, Zeyu Liu, Fan Zhang, Rong Sun, Yingjun Chen, Jun Li, Gan Zhang. Characterization of the nitrogen stable isotope composition (
δ
15
N) of ship-emitted NO
x
. Atmospheric Chemistry and Physics 2023, 23
(19)
, 12851-12865. https://doi.org/10.5194/acp-23-12851-2023
- Adam Francis, Raja S. Ganeshram, Robyn E. Tuerena, Robert G. M. Spencer, Robert M. Holmes, Jennifer A. Rogers, Claire Mahaffey. Permafrost degradation and nitrogen cycling in Arctic rivers: insights from stable nitrogen isotope studies. Biogeosciences 2023, 20
(2)
, 365-382. https://doi.org/10.5194/bg-20-365-2023
- Nicole M. Travis, Colette L. Kelly, Margaret R. Mulholland, Karen L. Casciotti. Nitrite cycling in the primary nitrite maxima of the eastern tropical North Pacific. Biogeosciences 2023, 20
(2)
, 325-347. https://doi.org/10.5194/bg-20-325-2023
- Shaosong Zhen, Min Luo, Yang Shao, Diandou Xu, Lingling Ma. Application of Stable Isotope Techniques in Tracing the Sources of Atmospheric NOX and Nitrate. Processes 2022, 10
(12)
, 2549. https://doi.org/10.3390/pr10122549
- Hiroto Kawashima. Seasonal trends of the stable nitrogen isotope ratio in particulate nitrogen compounds and their gaseous precursors in Akita, Japan. Tellus B: Chemical and Physical Meteorology 2022, 71
(1)
, 1627846. https://doi.org/10.1080/16000889.2019.1627846
- Margot E. White, Patrick A. Rafter, Brandon M. Stephens, Matthew R. Mazloff, Scott D. Wankel, Lihini I. Aluwihare. Stable isotopes of nitrate record effects of the 2015–2016 El Niño and diatom iron limitation on nitrogen cycling in the eastern North Pacific Ocean. Limnology and Oceanography 2022, 67
(10)
, 2140-2156. https://doi.org/10.1002/lno.12194
- Xin-Chu Wang, Fu-Jun Yue, Si-Liang Li, Xiao-Zheng Li, Yun-Chao Lang, Jian Hu, Hu Ding, Cong-Qiang Liu. Spatial variations in water chemical components in a coastal zone of northern China: Insights from environmental isotopes. Journal of Hydrology 2022, 612 , 128054. https://doi.org/10.1016/j.jhydrol.2022.128054
- Yasheng Shi, Yuming Hu, Zanfang Jin, Jiawen Li, Junfeng Zhang, Feili Li. Nitrate sources and its formation in precipitation during typhoons (In-fa and Chanthu) in multiple cities, East China. Science of The Total Environment 2022, 838 , 155949. https://doi.org/10.1016/j.scitotenv.2022.155949
- Alexandra Auderset, Simone Moretti, Björn Taphorn, Pia-Rebecca Ebner, Emma Kast, Xingchen T. Wang, Ralf Schiebel, Daniel M. Sigman, Gerald H. Haug, Alfredo Martínez-García. Enhanced ocean oxygenation during Cenozoic warm periods. Nature 2022, 609
(7925)
, 77-82. https://doi.org/10.1038/s41586-022-05017-0
- Shihui Wang, Yukun Ma, Xiaoyue Zhang, Yu Yu, Xuehui Zhou, Zhenyao Shen. Nitrogen transport and sources in urban stormwater with different rainfall characteristics. Science of The Total Environment 2022, 837 , 155902. https://doi.org/10.1016/j.scitotenv.2022.155902
- Angela N Knapp, Rachel K Thomas, Michael R Stukel, Thomas B Kelly, Michael R Landry, Karen E Selph, Estrella Malca, Trika Gerard, John Lamkin, . Constraining the sources of nitrogen fueling export production in the Gulf of Mexico using nitrogen isotope budgets. Journal of Plankton Research 2022, 44
(5)
, 692-710. https://doi.org/10.1093/plankt/fbab049
- Patrick J. Monreal, Colette L. Kelly, Nicole M. Travis, Karen L. Casciotti. Identifying the Sources and Drivers of Nitrous Oxide Accumulation in the Eddy‐Influenced Eastern Tropical North Pacific Oxygen‐Deficient Zone. Global Biogeochemical Cycles 2022, 36
(6)
https://doi.org/10.1029/2022GB007310
- Zhong-Jun Wang, Fu-Jun Yue, Yu-Chun Wang, Cai-Qing Qin, Hu Ding, Li-Li Xue, Si-Liang Li. The effect of heavy rainfall events on nitrogen patterns in agricultural surface and underground streams and the implications for karst water quality protection. Agricultural Water Management 2022, 266 , 107600. https://doi.org/10.1016/j.agwat.2022.107600
- Leonard I. Wassenaar, Simon D. Kelly, Cedric Douence, Marivil Islam, Lucilena Monteiro, Aiman Abrahim, Peter Rinke. Assessment of rapid low‐cost isotope (
δ
15
N,
δ
18
O) analyses of nitrate in fruit extracts by Ti(III) reduction to differentiate organic from conventional production. Rapid Communications in Mass Spectrometry 2022, 36
(7)
https://doi.org/10.1002/rcm.9259
- Qiyue Hu, Song Zhu, Zanfang Jin, Aijing Wu, Xiaoyu Chen, Feili Li. Using multiple isotopes to identify sources and transport of nitrate in urban residential stormwater runoff. Environmental Monitoring and Assessment 2022, 194
(4)
https://doi.org/10.1007/s10661-022-09763-6
- Zhong-Jun Wang, Fu-Jun Yue, Ji Lu, Yu-Chun Wang, Cai-Qing Qin, Hu Ding, Li-Li Xue, Si-Liang Li. New insight into the response and transport of nitrate in karst groundwater to rainfall events. Science of The Total Environment 2022, 818 , 151727. https://doi.org/10.1016/j.scitotenv.2021.151727
- N. Lehmann, M. Kienast, J. Granger, J.‐É. Tremblay. Physical and Biogeochemical Influences on Nutrients Through the Canadian Arctic Archipelago: Insights From Nitrate Isotope Ratios. Journal of Geophysical Research: Oceans 2022, 127
(3)
https://doi.org/10.1029/2021JC018179
- Mengyang Zhou, Julie Granger, Bonnie X. Chang. Influence of sample volume on nitrate N and O isotope ratio analyses with the denitrifier method. Rapid Communications in Mass Spectrometry 2022, 36
(4)
https://doi.org/10.1002/rcm.9224
- Shihui Wang, Yukun Ma, Xiaoyue Zhang, Zhenyao Shen. Transport and sources of nitrogen in stormwater runoff at the urban catchment scale. Science of The Total Environment 2022, 806 , 150281. https://doi.org/10.1016/j.scitotenv.2021.150281
- Yao Ding, Qing Shi, Lili OuYang, Bo Lai, Chengyue Lai, Gang Yao, Zhaoli Wang, Binyang Jia. Isotopic source identification of nitrogen pollution in the Pi River in Chengdu. Integrated Environmental Assessment and Management 2022, 18
(6)
, 1609-1620. https://doi.org/10.1002/ieam.4589
- Yasheng Shi, Ping Tian, Zanfang Jin, Yuming Hu, Yongqi Zhang, Feili Li. Stable nitrogen isotope composition of NOx of biomass burning in China. Science of The Total Environment 2022, 803 , 149857. https://doi.org/10.1016/j.scitotenv.2021.149857
- Shihui Wang, Yukun Ma, Zhenyao Shen. Nitrogen Transport and Sources in Urban Stormwater with Different Rainfall Characteristics. SSRN Electronic Journal 2022, 223 https://doi.org/10.2139/ssrn.4062575
- Marta Santos-Garcia, Raja S. Ganeshram, Robyn E. Tuerena, Margot C. F. Debyser, Katrine Husum, Philipp Assmy, Haakon Hop. Nitrate isotope investigations reveal future impacts of climate change on nitrogen inputs and cycling in Arctic fjords: Kongsfjorden and Rijpfjorden (Svalbard). Biogeosciences 2022, 19
(24)
, 5973-6002. https://doi.org/10.5194/bg-19-5973-2022
- Mhlangabezi Mdutyana, Tanya Marshall, Xin Sun, Jessica M. Burger, Sandy J. Thomalla, Bess B. Ward, Sarah E. Fawcett. Controls on nitrite oxidation in the upper Southern Ocean: insights from winter kinetics experiments in the Indian sector. Biogeosciences 2022, 19
(14)
, 3425-3444. https://doi.org/10.5194/bg-19-3425-2022
- Shantelle Smith, Katye E. Altieri, Mhlangabezi Mdutyana, David R. Walker, Ruan G. Parrott, Sedick Gallie, Kurt A. M. Spence, Jessica M. Burger, Sarah E. Fawcett. Biogeochemical controls on ammonium accumulation in the surface layer of the Southern Ocean. Biogeosciences 2022, 19
(3)
, 715-741. https://doi.org/10.5194/bg-19-715-2022
- Saehee Lim, Meehye Lee, Joel Savarino, Paolo Laj. Oxidation pathways and emission sources of atmospheric particulate nitrate in Seoul: based on
δ
15
N and Δ
17
O measurements. Atmospheric Chemistry and Physics 2022, 22
(8)
, 5099-5115. https://doi.org/10.5194/acp-22-5099-2022
- Yao Ding, Chengyue Lai, Qing Shi, Lili Ouyang, Zhaoli Wang, Gang Yao, Binyang Jia. Responses of Net Anthropogenic N Inputs and Export Fluxes in the Megacity of Chengdu, China. Water 2021, 13
(24)
, 3543. https://doi.org/10.3390/w13243543
- Wei Wei, Kazuo Isobe, Yutaka Shiratori, Midori Yano, Sakae Toyoda, Keisuke Koba, Naohiro Yoshida, Haoyang Shen, Keishi Senoo. Revisiting the involvement of ammonia oxidizers and denitrifiers in nitrous oxide emission from cropland soils. Environmental Pollution 2021, 287 , 117494. https://doi.org/10.1016/j.envpol.2021.117494
- Roisin McCallum, Bradley Eyre, Glenn Hyndes, Kathryn McMahon, Joanne M. Oakes, Naomi S. Wells. Importance of internal dissolved organic nitrogen loading and cycling in a small and heavily modified coastal lagoon. Biogeochemistry 2021, 155
(2)
, 237-261. https://doi.org/10.1007/s10533-021-00824-5
- Jesse R. Farmer, Daniel M. Sigman, Julie Granger, Ona M. Underwood, François Fripiat, Thomas M. Cronin, Alfredo Martínez-García, Gerald H. Haug. Arctic Ocean stratification set by sea level and freshwater inputs since the last ice age. Nature Geoscience 2021, 14
(9)
, 684-689. https://doi.org/10.1038/s41561-021-00789-y
- Ute Risse-Buhl, Christine Anlanger, Christian Noss, Andreas Lorke, Daniel von Schiller, Markus Weitere. Hydromorphologic Sorting of In-Stream Nitrogen Uptake Across Spatial Scales. Ecosystems 2021, 24
(5)
, 1184-1202. https://doi.org/10.1007/s10021-020-00576-7
- Naomi S. Wells, Bradley Eyre. Flow regulates biological NO3− and N2O production in a turbid sub-tropical stream. Geochimica et Cosmochimica Acta 2021, 306 , 124-142. https://doi.org/10.1016/j.gca.2021.05.026
- Keisuke Koba, Kei Kinoshita, Yuji Onishi, Keitaro Fukushima, Ken’ichi Osaka, Naoko Matsuo, Kazuho Funakawa, Yugo Seko, Ayano Medo, Riyo Hirasawa, Nanako O Ogawa, Fujio Hyodo, Chikage Yoshimizu. Carbon and Nitrogen Isotope Analysis on Small Samples Using a Near-conventional EA-IRMS System. RADIOISOTOPES 2021, 70
(4)
, 291-299. https://doi.org/10.3769/radioisotopes.70.291
- Lin Zhang, Wing‐man (Charlotte) Lee, Ava Kreider‐Mueller, Evelyn Kuhnel, Jesus Baca, Chongxiao Ji, Mark Altabet. High‐precision measurement of phenylalanine and glutamic acid δ
15
N by coupling ion‐exchange chromatography and purge‐and‐trap continuous‐flow isotope ratio mass spectrometry. Rapid Communications in Mass Spectrometry 2021, 35
(13)
https://doi.org/10.1002/rcm.9085
- Yasheng Shi, Cai Li, Zanfang Jin, Yongqi Zhang, Jiazheng Xiao, Feili Li. Combining dual isotopes and a Bayesian isotope mixing model to quantify the nitrate sources of precipitation in Ningbo, East China. Science of The Total Environment 2021, 778 , 146297. https://doi.org/10.1016/j.scitotenv.2021.146297
- Sai-Nan Chen, Fu-Jun Yue, Xiao-Long Liu, Jun Zhong, Yuan-Bi Yi, Wan-Fa Wang, Yulin Qi, Hua-Yun Xiao, Si-Liang Li. Seasonal variation of nitrogen biogeochemical processes constrained by nitrate dual isotopes in cascade reservoirs, Southwestern China. Environmental Science and Pollution Research 2021, 28
(21)
, 26617-26627. https://doi.org/10.1007/s11356-021-12505-9
- Sarah M. Laperriere, Michael Morando, Douglas G. Capone, Troy Gunderson, Jason M. Smith, Alyson E. Santoro. Nitrification and nitrous oxide dynamics in the Southern California Bight. Limnology and Oceanography 2021, 66
(4)
, 1099-1112. https://doi.org/10.1002/lno.11667
- Kalina C. Grabb, Shuai Ding, Xiaoyan Ning, Su Mei Liu, Bao Qian. Characterizing the impact of Three Gorges Dam on the Changjiang (Yangtze River): A story of nitrogen biogeochemical cycling through the lens of nitrogen stable isotopes. Environmental Research 2021, 195 , 110759. https://doi.org/10.1016/j.envres.2021.110759
- Hiroto Kawashima, Otoha Yoshida, Nana Suto. Ion‐exchange resin and denitrification pretreatment for determining δ
15
N‐NH
4
+
, δ
15
N‐NO
3
−
, and δ
18
O‐NO
3
−
values. Rapid Communications in Mass Spectrometry 2021, 35
(6)
https://doi.org/10.1002/rcm.9027
- Fabio Marzaioli, Brunella Di Rienzo, Luisa Stellato, Egidio Di Fusco, Mauro Rubino, Antonio D'Onofrio, Filippo Terrasi. Characterization of the analytical performance of δ
15
N and δ
18
O measurements by the silver nitrate method in the framework of nitrate source apportioning. Rapid Communications in Mass Spectrometry 2021, 35
(5)
https://doi.org/10.1002/rcm.9009
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.