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Technical Updates to the Bacterial Method for Nitrate Isotopic Analyses
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    Technical Updates to the Bacterial Method for Nitrate Isotopic Analyses
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    Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, 360 Woods Hole Road, MS 52, Woods Hole, Massachusetts 02543, United States
    *E-mail: [email protected]. Phone: 650-721-5545.
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    Analytical Chemistry

    Cite this: Anal. Chem. 2011, 83, 5, 1850–1856
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    https://doi.org/10.1021/ac1028984
    Published February 8, 2011
    Copyright © 2011 American Chemical Society

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    The bacterial conversion of aqueous nitrate (NO3) to nitrous oxide (N2O) for isotopic analysis has found widespread use since its introduction (Sigman, D. M.; Casciotti, K. L.; Andreani, M.; Galanter, M.; Böhlke, J. K. Anal. Chem.2001, 73, 4145−4153; Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905−4912). The bacterial strain Pseudomonas aureofaciens (ATTC no. 13985) was shown to convert NO3 to N2O while retaining both N and O isotopic signatures, and automation of the isotopic analysis of N2O greatly increased the throughput of the method (Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905−4912). Continued development of the denitrifier method has led to increased precision and throughput of NO3 isotopic analysis. Presented here are several recent procedural modifications and the demonstration of their effectiveness.

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    Cite this: Anal. Chem. 2011, 83, 5, 1850–1856
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    https://doi.org/10.1021/ac1028984
    Published February 8, 2011
    Copyright © 2011 American Chemical Society

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