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Chromatographic Determination of Nanomolar Cyanate Concentrations in Estuarine and Sea Waters by Precolumn Fluorescence Derivatization

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    Chromatographic Determination of Nanomolar Cyanate Concentrations in Estuarine and Sea Waters by Precolumn Fluorescence Derivatization
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    Department of Ocean, Earth and Atmospheric Sciences, Old Dominion University, 4600 Elkhorn Ave. Norfolk, Virginia 23529, United States
    *Phone: (757) 683-5989. Fax: (757) 683-5303. E-mail: [email protected]
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    Analytical Chemistry

    Cite this: Anal. Chem. 2013, 85, 14, 6661–6666
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    https://doi.org/10.1021/ac400351c
    Published June 6, 2013
    Copyright © 2013 American Chemical Society
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    Abstract

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    Recent studies suggest that cyanate (OCN–) is a potentially important source of reduced nitrogen (N) available to support the growth of aquatic microbes and, thus, may play a role in aquatic N cycling. However, aquatic OCN– distributions have not been previously described because of the lack of a suitable assay for measuring OCN– concentrations in natural waters. Previous methods were designed to quantify OCN– in aqueous samples with much higher reduced N concentrations (micromolar levels) than those likely to be found in natural waters (nanomolar levels). We have developed a method to quantify OCN– in dilute, saline environments. In the method described here, OCN– in aqueous solution reacts with 2-aminobenzoic acid to produce a highly fluorescent derivative, 2,4-quinazolinedione, which is then quantified using high performance liquid chromatography. Derivatization conditions were optimized to simultaneously minimize the reagent blank and maximize 2,4-quinazolinedione formation (>90% reaction yield) in estuarine and seawater matrices. A limit of detection (LOD) of 0.4 nM was achieved with only minor matrix effects. We applied this method to measure OCN– concentrations in estuarine and seawater samples from the Chesapeake Bay and coastal waters from the mid-Atlantic region. OCN– concentrations ranged from 0.9 to 41 nM. We determined that OCN– concentrations were stable in 0.2 μm filtered seawater samples stored at −80 °C for up to nine months.

    Copyright © 2013 American Chemical Society

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    This article is cited by 34 publications.

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    Analytical Chemistry

    Cite this: Anal. Chem. 2013, 85, 14, 6661–6666
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ac400351c
    Published June 6, 2013
    Copyright © 2013 American Chemical Society
    Request reuse permissions

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