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Direct Replacement of Antibodies with Molecularly Imprinted Polymer Nanoparticles in ELISA—Development of a Novel Assay for Vancomycin

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Cranfield Health, Vincent Building, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, U.K.
*E-mail: [email protected]. Tel.: +44 1234 758322. Fax: +44 1234 758380.
Cite this: Anal. Chem. 2013, 85, 17, 8462–8468
Publication Date (Web):August 15, 2013
https://doi.org/10.1021/ac402102j
Copyright © 2013 American Chemical Society

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    Abstract

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    A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop assays similar to the enzyme-linked immunosorbent assay (ELISA) is presented here for the first time. NanoMIPs were synthesized by a solid-phase approach with an immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering, and electron microscopy. Immobilization, blocking, and washing conditions were optimized in microplate format. The detection of vancomycin was achieved in competitive binding experiments with a horseradish peroxidase–vancomycin conjugate. The assay was capable of measuring vancomycin in buffer and in blood plasma within the range of 0.001–70 nM with a detection limit of 0.0025 nM (2.5 pM). The sensitivity of the assay was 3 orders of magnitude better than a previously described ELISA based on antibodies. In these experiments, nanoMIPs have shown high affinity and minimal interference from blood plasma components. Immobilized nanoMIPs were stored for 1 month at room temperature without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELISA.

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    Figures showing the Biacore testing to estimate dissociation constant of nanoMIPs, the calibration curves of HRP–V and HRP for dilutions of TMB reagent, and the results of experiments carried out to optimize washing buffer and quantity of nanoparticles per well are supplied as Supporting Information. This material is available free of charge via the Internet at http://pubs.acs.org.

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    8. Shue Mei Ng, Xiangyang Wu, M. Faisal Khyasudeen, Paweł J. Nowakowski, Howe-Siang Tan, Bengang Xing, Edwin K. L. Yeow. Vancomycin Determination by Disrupting Electron-Transfer in a Fluorescence Turn-On Squaraine–Anthraquinone Triad. ACS Sensors 2018, 3 (6) , 1156-1163. https://doi.org/10.1021/acssensors.8b00188
    9. Chaochao Chen, Jiaxun Luo, Chenglong Li, Mingfang Ma, Wenbo Yu, Jianzhong Shen, Zhanhui Wang. Molecularly Imprinted Polymer as an Antibody Substitution in Pseudo-immunoassays for Chemical Contaminants in Food and Environmental Samples. Journal of Agricultural and Food Chemistry 2018, 66 (11) , 2561-2571. https://doi.org/10.1021/acs.jafc.7b05577
    10. Zhuyuan Wang, Shenfei Zong, Lei Wu, Dan Zhu, and Yiping Cui . SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications. Chemical Reviews 2017, 117 (12) , 7910-7963. https://doi.org/10.1021/acs.chemrev.7b00027
    11. Hanne Diliën, Marloes Peeters, Jeroen Royakkers, Jules Harings, Peter Cornelis, Patrick Wagner, Erik Steen Redeker, Craig E. Banks, Kasper Eersels, Bart van Grinsven, and Thomas J. Cleij . Label-Free Detection of Small Organic Molecules by Molecularly Imprinted Polymer Functionalized Thermocouples: Toward In Vivo Applications. ACS Sensors 2017, 2 (4) , 583-589. https://doi.org/10.1021/acssensors.7b00104
    12. Takamasa Kinoshita, Dung Q. Nguyen, Dung Q. Le, Kengo Ishiki, Hiroshi Shiigi, and Tsutomu Nagaoka . Shape Memory Characteristics of O157-Antigenic Cavities Generated on Nanocomposites Consisting of Copolymer-Encapsulated Gold Nanoparticles. Analytical Chemistry 2017, 89 (8) , 4680-4684. https://doi.org/10.1021/acs.analchem.7b00308
    13. Maddalena Bertolla, Lucia Cenci, Andrea Anesi, Emmanuele Ambrosi, Franco Tagliaro, Lia Vanzetti, Graziano Guella, and Alessandra Maria Bossi . Solvent-Responsive Molecularly Imprinted Nanogels for Targeted Protein Analysis in MALDI-TOF Mass Spectrometry. ACS Applied Materials & Interfaces 2017, 9 (8) , 6908-6915. https://doi.org/10.1021/acsami.6b16291
    14. Joana Rafaela Lara Guerreiro, Vladimir E. Bochenkov, Kasper Runager, Hüsnü Aslan, Mingdong Dong, Jan J. Enghild, Victor De Freitas, Maria Goreti Ferreira Sales, and Duncan S. Sutherland . Molecular Imprinting of Complex Matrices at Localized Surface Plasmon Resonance Biosensors for Screening of Global Interactions of Polyphenols and Proteins. ACS Sensors 2016, 1 (3) , 258-264. https://doi.org/10.1021/acssensors.5b00054
    15. Judith Wackerlig and Romana Schirhagl . Applications of Molecularly Imprinted Polymer Nanoparticles and Their Advances toward Industrial Use: A Review. Analytical Chemistry 2016, 88 (1) , 250-261. https://doi.org/10.1021/acs.analchem.5b03804
    16. Tianwei Lv, Hongyuan Yan, Jiankun Cao, and Shiru Liang . Hydrophilic Molecularly Imprinted Resorcinol–Formaldehyde–Melamine Resin Prepared in Water with Excellent Molecular Recognition in Aqueous Matrices. Analytical Chemistry 2015, 87 (21) , 11084-11091. https://doi.org/10.1021/acs.analchem.5b03253
    17. Alessandro Poma, Heli Brahmbhatt, Jonathan K. Watts, and Nicholas W. Turner . Nucleoside-Tailored Molecularly Imprinted Polymeric Nanoparticles (MIP NPs). Macromolecules 2014, 47 (18) , 6322-6330. https://doi.org/10.1021/ma501530c
    18. Yonghuan He, Yanyan Huang, Yulong Jin, Xiangjun Liu, Guoquan Liu, and Rui Zhao . Well-Defined Nanostructured Surface-Imprinted Polymers for Highly Selective Magnetic Separation of Fluoroquinolones in Human Urine. ACS Applied Materials & Interfaces 2014, 6 (12) , 9634-9642. https://doi.org/10.1021/am5020666
    19. Rahim Khan, Farooq Anwar, Farinazleen Mohamad Ghazali. A comprehensive review of mycotoxins: Toxicology, detection, and effective mitigation approaches. Heliyon 2024, 85 , e28361. https://doi.org/10.1016/j.heliyon.2024.e28361
    20. Cem Esen, Francesco Canfarotta. Molecularly imprinted polymer nanoparticles: solid-phase synthesis and molecularly imprinted nanoparticle assay. 2024, 93-106. https://doi.org/10.1016/B978-0-443-15359-4.00004-8
    21. Sobhi Daniel, K.S. Sunish. Intelligent molecularly imprinted polymeric gels for biomedical applications. 2024, 99-124. https://doi.org/10.1016/B978-0-12-823135-7.00007-3
    22. Amy Dann, Sarbjeet Kaur, Sloane Stoufer, Minji Kim, Inderpreet Kaur, Matthew D. Moore, Marloes Peeters, Jake McClements. Molecularly Imprinted Polymers for Detection of Chemical and Microbial Contaminants in Foods. 2024, 303-314. https://doi.org/10.1016/B978-0-12-822521-9.00186-6
    23. Raphael D. Ayivi, Sherine O. Obare, Jianjun Wei. Molecularly imprinted polymers as chemosensors for organophosphate pesticide detection and environmental applications. TrAC Trends in Analytical Chemistry 2023, 167 , 117231. https://doi.org/10.1016/j.trac.2023.117231
    24. Fabio Di Nardo, Laura Anfossi, Claudio Baggiani. MIP-based immunoassays: A critical review. Analytica Chimica Acta 2023, 1277 , 341547. https://doi.org/10.1016/j.aca.2023.341547
    25. Xu Ni, Xue Tang, Dan Wang, Jingjing Zhang, Linjie Zhao, Jie Gao, Hua He, Pierre Dramou. Research progress of sensors based on molecularly imprinted polymers in analytical and biomedical analysis. Journal of Pharmaceutical and Biomedical Analysis 2023, 235 , 115659. https://doi.org/10.1016/j.jpba.2023.115659
    26. Rina Arad Yellin, Anett Hudák, Tamás Letoha, Bernard S. Green. Tumor necrosis factor alpha (TNFα)-binding imprinted polymers. A non-systemic therapeutic approach for inflammatory bowel diseases. European Journal of Medicinal Chemistry Reports 2023, 8 , 100106. https://doi.org/10.1016/j.ejmcr.2023.100106
    27. Rong Ding, Mengxue Ye, Yijie Zhu, Yingyan Zhao, Qi Liu, Ya Cao, Jingjing Xu. Toward Dynamic Detection of Circulating Tumor Cells Exploiting Specific Molecular Recognition Elements. Chemosensors 2023, 11 (2) , 99. https://doi.org/10.3390/chemosensors11020099
    28. Yadiris García, Myleidi Vera, Verónica A. Jiménez, Luis F. Barraza, Joao Aguilar, Susana Sánchez, Eduardo D. Pereira. Molecularly imprinted nanoparticle-based assay (MINA) for microcystin-LR detection in water. The Analyst 2023, 148 (2) , 305-315. https://doi.org/10.1039/D2AN01680J
    29. Aabha Bajaj, Michelle Buchholz, Sunil Choudhary, Zeynep Altintas. Molecularly imprinted polymer sensors. 2023, 475-504. https://doi.org/10.1016/B978-0-323-88431-0.00008-9
    30. Xiaorong Zhang, Aysu Yarman, Mahdien Bagheri, Ibrahim M. El-Sherbiny, Rabeay Y. A. Hassan, Sevinc Kurbanoglu, Armel Franklin Tadjoung Waffo, Ingo Zebger, Tutku Ceren Karabulut, Frank F. Bier, Peter Lieberzeit, Frieder W. Scheller. Imprinted Polymers on the Route to Plastibodies for Biomacromolecules (MIPs), Viruses (VIPs), and Cells (CIPs). 2023https://doi.org/10.1007/10_2023_234
    31. Sona Bakhshizadeh, Mohammad Mehdi Sabzehmeidani, Mehrorang Ghaedi, Kheibar Dashtian, Hamid Abbasi-Asl. Preparation and characterization of mixed matrix membranes based on PVDF blend and hydrophilic molecularly imprinted MIL-101 (Cr) as filler for efficient selective removal of dye. Journal of Environmental Chemical Engineering 2022, 10 (6) , 108864. https://doi.org/10.1016/j.jece.2022.108864
    32. Valentina Testa, Laura Anfossi, Simone Cavalera, Matteo Chiarello, Fabio Di Nardo, Thea Serra, Claudio Baggiani. Effect of Surfactants on the Binding Properties of a Molecularly Imprinted Polymer. Polymers 2022, 14 (23) , 5210. https://doi.org/10.3390/polym14235210
    33. César Cáceres, Macarena del Pilar Garcia Morgado, Freddy Celis Bozo, Sergey Piletsky, Ewa Moczko. Rapid Selective Detection and Quantification of β-Blockers Used in Doping Based on Molecularly Imprinted Nanoparticles (NanoMIPs). Polymers 2022, 14 (24) , 5420. https://doi.org/10.3390/polym14245420
    34. Xin Cheng, Jingxin Ma, Jianrong Su. An Overview of Analytical Methodologies for Determination of Vancomycin in Human Plasma. Molecules 2022, 27 (21) , 7319. https://doi.org/10.3390/molecules27217319
    35. Ahmed R. Mohamed. Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment. Journal of Fluorescence 2022, 32 (5) , 1899-1912. https://doi.org/10.1007/s10895-022-02942-1
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    37. Francesca Torrini, Laura Caponi, Andrea Bertolini, Pasquale Palladino, Francesca Cipolli, Alessandro Saba, Aldo Paolicchi, Simona Scarano, Maria Minunni. A biomimetic enzyme-linked immunosorbent assay (BELISA) for the analysis of gonadorelin by using molecularly imprinted polymer-coated microplates. Analytical and Bioanalytical Chemistry 2022, 414 (18) , 5423-5434. https://doi.org/10.1007/s00216-021-03867-7
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    39. Ortensia Ilaria Parisi, Fabrizio Francomano, Marco Dattilo, Francesco Patitucci, Sabrina Prete, Fabio Amone, Francesco Puoci. The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart. Journal of Functional Biomaterials 2022, 13 (1) , 12. https://doi.org/10.3390/jfb13010012
    40. Yanru Wang, Cui Zhang, Jianlong Wang, Dietmar Knopp. Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review. Toxins 2022, 14 (2) , 73. https://doi.org/10.3390/toxins14020073
    41. M.A. Goicolea, A. Gómez-Caballero, M. Saumell-Esnaola, G. García del Caño, N. Unceta, J. Sallés, R.J. Barrio. A linear-polymer-based lactoferrin-selective recognition element for an ELISA mimic: A proof of concept. Analytica Chimica Acta 2022, 1191 , 339309. https://doi.org/10.1016/j.aca.2021.339309
    42. Yadiris García, Joanna Czulak, Eduardo D. Pereira, Sergey A. Piletsky, Elena Piletska. A magnetic molecularly imprinted nanoparticle assay (MINA) for detection of pepsin. Reactive and Functional Polymers 2022, 170 , 105133. https://doi.org/10.1016/j.reactfunctpolym.2021.105133
    43. Sanjana Naveen Prasad, Vipul Bansal, Rajesh Ramanathan. Detection of pesticides using nanozymes: Trends, challenges and outlook. TrAC Trends in Analytical Chemistry 2021, 144 , 116429. https://doi.org/10.1016/j.trac.2021.116429
    44. Maylis Garnier, Michèle Sabbah, Christine Ménager, Nébéwia Griffete. Hybrid Molecularly Imprinted Polymers: The Future of Nanomedicine?. Nanomaterials 2021, 11 (11) , 3091. https://doi.org/10.3390/nano11113091
    45. Matteo Chiarello, Laura Anfossi, Simone Cavalera, Fabio Di Nardo, Thea Serra, Claudio Baggiani. NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study. Separations 2021, 8 (11) , 226. https://doi.org/10.3390/separations8110226
    46. Camila Quezada, Myleidi Vera, Luis F. Barraza, Yadiris García, Eduardo D. Pereira. Molecularly imprinted nanoparticle-based assay (MINA): Potential application for the detection of the neurotoxin domoic acid. Analytica Chimica Acta 2021, 1181 , 338887. https://doi.org/10.1016/j.aca.2021.338887
    47. Matteo Chiarello, Laura Anfossi, Simone Cavalera, Fabio Di Nardo, Fiora Artusio, Roberto Pisano, Claudio Baggiani. Effect of Polymerization Time on the Binding Properties of Ciprofloxacin-Imprinted nanoMIPs Prepared by Solid-Phase Synthesis. Polymers 2021, 13 (16) , 2656. https://doi.org/10.3390/polym13162656
    48. Oliver Jamieson, Francesco Mecozzi, Robert D. Crapnell, William Battell, Alexander Hudson, Katarina Novakovic, Ashwin Sachdeva, Francesco Canfarotta, Carmelo Herdes, Craig E. Banks, Helena Snyder, Marloes Peeters. Approaches to the Rational Design of Molecularly Imprinted Polymers Developed for the Selective Extraction or Detection of Antibiotics in Environmental and Food Samples. physica status solidi (a) 2021, 218 (13) https://doi.org/10.1002/pssa.202100021
    49. Simone Cavalera, Matteo Chiarello, Fabio Di Nardo, Laura Anfossi, Claudio Baggiani. Effect of experimental conditions on the binding abilities of ciprofloxacin-imprinted nanoparticles prepared by solid-phase synthesis. Reactive and Functional Polymers 2021, 163 , 104893. https://doi.org/10.1016/j.reactfunctpolym.2021.104893
    50. LiangLiang Liu, Fabiana Grillo, Francesco Canfarotta, Michael Whitcombe, Stephen P. Morgan, Sergey Piletsky, Ricardo Correia, ChenYang He, Andrew Norris, Serhiy Korposh. Carboxyl-fentanyl detection using optical fibre grating-based sensors functionalised with molecularly imprinted nanoparticles. Biosensors and Bioelectronics 2021, 177 , 113002. https://doi.org/10.1016/j.bios.2021.113002
    51. Fangya Mu, Junqing He, Fang Fan, Guoyue Shi. Dual-emission fluorescence biosensing of vancomycin based on AIEgen–peptide conjugates and aptamer-modified Au nanoclusters. Analytica Chimica Acta 2021, 1150 , 238177. https://doi.org/10.1016/j.aca.2020.12.052
    52. Aysu Yarman, Sevinc Kurbanoglu, Ingo Zebger, Frieder W. Scheller. Simple and robust: The claims of protein sensing by molecularly imprinted polymers. Sensors and Actuators B: Chemical 2021, 330 , 129369. https://doi.org/10.1016/j.snb.2020.129369
    53. Chengya Dong, Hongxing Shi, Yuanrui Han, Yuanyuan Yang, Ruixin Wang, Jiying Men. Molecularly imprinted polymers by the surface imprinting technique. European Polymer Journal 2021, 145 , 110231. https://doi.org/10.1016/j.eurpolymj.2020.110231
    54. César Cáceres, Ewa Moczko, Itsaso Basozabal, Antonio Guerreiro, Sergey Piletsky. Molecularly Imprinted Nanoparticles (NanoMIPs) Selective for Proteins: Optimization of a Protocol for Solid-Phase Synthesis Using Automatic Chemical Reactor. Polymers 2021, 13 (3) , 314. https://doi.org/10.3390/polym13030314
    55. Alberto Gómez-Caballero, Nora Unceta, M. Aránzazu Goicolea, Ramón J. Barrio. Plastic Receptors Developed by Imprinting Technology as Smart Polymers Imitating Natural Behavior. 2021, 69-116. https://doi.org/10.1007/978-3-030-50457-1_5
    56. Sanjana Naveen Prasad, Vipul Bansal, Rajesh Ramanathan. Nanozyme-Based Sensors for Pesticide Detection. 2021, 145-175. https://doi.org/10.1007/978-3-030-68230-9_6
    57. Li Ma, Zhaosheng Liu. MIP as Drug Delivery Systems for Special Application. 2021, 179-200. https://doi.org/10.1007/978-981-16-0227-6_9
    58. Aysu Yarman, Sevinc Kurbanoglu, Cem Erkmen, Bengi Uslu, Frieder W. Scheller. Quantum dot-based electrochemical molecularly imprinted polymer sensors: potentials and challenges. 2021, 121-153. https://doi.org/10.1016/B978-0-12-821670-5.00013-0
    59. Hichem Moulahoum, Faezeh Ghorbanizamani, Figen Zihnioglu, Suna Timur. Tracking and Treating: Molecularly Imprinted Polymer-Based Nanoprobes Application in Theranostics. 2021, 45-68. https://doi.org/10.1016/B978-0-12-822117-4.00003-4
    60. Yanhua Sun, Chuan Yao, Zhixu Xie, Yange Zhang. Lotus seedpod-like molecularly imprinted polymers fabricated by MOF-808 stabilized Pickering emulsion and their specific recognition of hemoglobin. Colloids and Surfaces B: Biointerfaces 2021, 197 , 111446. https://doi.org/10.1016/j.colsurfb.2020.111446
    61. Suticha Chunta, Worachote Boonsriwong, Panwadee Wattanasin, Wanpen Naklua, Peter A. Lieberzeit. Direct assessment of very-low-density lipoprotein by mass sensitive sensor with molecularly imprinted polymers. Talanta 2021, 221 , 121549. https://doi.org/10.1016/j.talanta.2020.121549
    62. Mashaalah Zarejousheghani, Alaa Jaafar, Hendrik Wollmerstaedt, Parvaneh Rahimi, Helko Borsdorf, Stefan Zimmermann, Yvonne Joseph. Rational Design of Molecularly Imprinted Polymers Using Quaternary Ammonium Cations for Glyphosate Detection. Sensors 2021, 21 (1) , 296. https://doi.org/10.3390/s21010296
    63. Nasim Sanadgol, Judith Wackerlig. Developments of Smart Drug-Delivery Systems Based on Magnetic Molecularly Imprinted Polymers for Targeted Cancer Therapy: A Short Review. Pharmaceutics 2020, 12 (9) , 831. https://doi.org/10.3390/pharmaceutics12090831
    64. Alejandra Sabín López, María Paredes Ramos, Roberto Herrero, Jose Manuel López Vilariño. Synthesis of magnetic green nanoparticle – Molecular imprinted polymers with emerging contaminants templates. Journal of Environmental Chemical Engineering 2020, 8 (4) , 103889. https://doi.org/10.1016/j.jece.2020.103889
    65. Nazia Tarannum, Olga D. Hendrickson, Shahjadi Khatoon, Anatoly V. Zherdev, Boris B. Dzantiev. Molecularly imprinted polymers as receptors for assays of antibiotics. Critical Reviews in Analytical Chemistry 2020, 50 (4) , 291-310. https://doi.org/10.1080/10408347.2019.1626697
    66. Jingjing Xu, Haohan Miao, Jixiang Wang, Guoqing Pan. Molecularly Imprinted Synthetic Antibodies: From Chemical Design to Biomedical Applications. Small 2020, 16 (27) https://doi.org/10.1002/smll.201906644
    67. Guilaine Jaria, Vânia Calisto, Marta Otero, Valdemar I. Esteves. Monitoring pharmaceuticals in the aquatic environment using enzyme-linked immunosorbent assay (ELISA)—a practical overview. Analytical and Bioanalytical Chemistry 2020, 412 (17) , 3983-4008. https://doi.org/10.1007/s00216-020-02509-8
    68. Yi Cai, Xin He, Peng Lei Cui, Wan Zhe Yuan, Jian Ping Wang, Jing Liu. Molecularly imprinted microspheres based multiplexed fluorescence method for simultaneous detection of benzimidazoles and pyrethroids in meat samples. Food Chemistry 2020, 319 , 126539. https://doi.org/10.1016/j.foodchem.2020.126539
    69. V. Baldoneschi, P. Palladino, M. Banchini, M. Minunni, S. Scarano. Norepinephrine as new functional monomer for molecular imprinting: An applicative study for the optical sensing of cardiac biomarkers. Biosensors and Bioelectronics 2020, 157 , 112161. https://doi.org/10.1016/j.bios.2020.112161
    70. Jingbo He, Li Zhang, Longhua Xu, Feifan Kong, Zhixiang Xu. Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods. Advances in Polymer Technology 2020, 2020 , 1-8. https://doi.org/10.1155/2020/7647580
    71. Jia-Huan Qu, Annelies Dillen, Wouter Saeys, Jeroen Lammertyn, Dragana Spasic. Advancements in SPR biosensing technology: An overview of recent trends in smart layers design, multiplexing concepts, continuous monitoring and in vivo sensing. Analytica Chimica Acta 2020, 1104 , 10-27. https://doi.org/10.1016/j.aca.2019.12.067
    72. Roberta D’Aurelio, Iva Chianella, Jack A. Goode, Ibtisam E. Tothill. Molecularly Imprinted Nanoparticles Based Sensor for Cocaine Detection. Biosensors 2020, 10 (3) , 22. https://doi.org/10.3390/bios10030022
    73. Y. Zhao, C. Simon, M. Daoud Attieh, K. Haupt, A. Falcimaigne-Cordin. Reduction-responsive molecularly imprinted nanogels for drug delivery applications. RSC Advances 2020, 10 (10) , 5978-5987. https://doi.org/10.1039/C9RA07512G
    74. Nelson Caro, Tamara Bruna, Antonio Guerreiro, Paola Alvarez-Tejos, Virginia Garretón, Sergey Piletsky, Jorge González-Casanova, Diana Rojas-Gómez, Nicole Ehrenfeld. Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples. Nanomaterials 2020, 10 (2) , 306. https://doi.org/10.3390/nano10020306
    75. Huiqi Zhang. Molecularly Imprinted Nanoparticles for Biomedical Applications. Advanced Materials 2020, 32 (3) https://doi.org/10.1002/adma.201806328
    76. Ortensia Ilaria Parisi, Mariarosa Ruffo, Francesco Puoci. Molecularly imprinted polymers for selective recognition in regenerative medicine. 2020, 141-163. https://doi.org/10.1016/B978-0-08-102594-9.00005-X
    77. Ian A. Nicholls, Jesper G. Wiklander. Towards Peptide and Protein Recognition by Antibody Mimicking Synthetic Polymers – Background, State of the Art, and Future Outlook. Australian Journal of Chemistry 2020, 73 (4) , 300. https://doi.org/10.1071/CH20020
    78. Tao Deng, Shiyou Hu, Liang Zhao, Shengjun Wu, Wei Liu, Tongkai Chen, Tingdan Fu, He Wang, Huifang Shi, Xin-an Huang, Fang Liu. A ratiometric fluorescent probe for sensitive determination of the important glycopeptide antibiotic vancomycin. Analytical and Bioanalytical Chemistry 2019, 411 (30) , 8103-8111. https://doi.org/10.1007/s00216-019-02190-6
    79. Asma Elbelazi, Francesco Canfarotta, Joanna Czulak, Michael J. Whitcombe, Sergey Piletsky, Elena Piletska. Development of a homogenous assay based on fluorescent imprinted nanoparticles for analysis of nitroaromatic compounds. Nano Research 2019, 12 (12) , 3044-3050. https://doi.org/10.1007/s12274-019-2550-1
    80. Lingli Zhou, Yijia Wang, Rongrong Xing, Jin Chen, Jia Liu, Wei Li, Zhen Liu. Orthogonal dual molecularly imprinted polymer-based plasmonic immunosandwich assay: A double characteristic recognition strategy for specific detection of glycoproteins. Biosensors and Bioelectronics 2019, 145 , 111729. https://doi.org/10.1016/j.bios.2019.111729
    81. Katarzyna Smolinska-Kempisty, Antonio Guerreiro, Joanna Czulak, Sergey Piletsky. Negative selection of MIPs to create high specificity ligands for glycated haemoglobin. Sensors and Actuators B: Chemical 2019, 301 , 126967. https://doi.org/10.1016/j.snb.2019.126967
    82. Doaa Refaat, Mohamed G. Aggour, Ahmed A. Farghali, Rashmi Mahajan, Jesper G. Wiklander, Ian A. Nicholls, Sergey A. Piletsky. Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications. International Journal of Molecular Sciences 2019, 20 (24) , 6304. https://doi.org/10.3390/ijms20246304
    83. Hasim Munawar, Abeer H.M. Safaryan, Annalisa De Girolamo, Alvaro Garcia-Cruz, Pedro Marote, Kal Karim, Vincenzo Lippolis, Michelangelo Pascale, Sergey A. Piletsky. Determination of Fumonisin B1 in maize using molecularly imprinted polymer nanoparticles-based assay. Food Chemistry 2019, 298 , 125044. https://doi.org/10.1016/j.foodchem.2019.125044
    84. Yadiris Garcia, Francesco Canfarotta, Katarzyna Smolinska-Kempisty, Sergey A. Piletsky, Eduardo Pereira. Competitive pseudo-ELISA based on molecularly imprinted nanoparticles for microcystin-LR detection in water. Pure and Applied Chemistry 2019, 91 (10) , 1593-1604. https://doi.org/10.1515/pac-2018-1207
    85. Run-tian Ma, Xiao-bo Zhao, Xiao-yu Sun, Jia Liu, Wei Ha, Yan-ping Shi. A fluorescent molecularly imprinted device for the on-line analysis of AFP in human serum. Journal of Materials Chemistry B 2019, 7 (40) , 6187-6194. https://doi.org/10.1039/C9TB01331H
    86. Abeer H. M. Safaryan, Adam M. Smith, Thomas S. Bedwell, Elena V. Piletska, Francesco Canfarotta, Sergey A. Piletsky. Optimisation of the preservation conditions for molecularly imprinted polymer nanoparticles specific for trypsin. Nanoscale Advances 2019, 1 (9) , 3709-3714. https://doi.org/10.1039/C9NA00327D
    87. Heba A. Hussein, Rabeay Y.A. Hassan, Rasha Mohamed El Nashar, Samy A. Khalil, Sayed A. Salem, Ibrahim M. El-Sherbiny. Designing and fabrication of new VIP biosensor for the rapid and selective detection of foot-and-mouth disease virus (FMDV). Biosensors and Bioelectronics 2019, 141 , 111467. https://doi.org/10.1016/j.bios.2019.111467
    88. Ewa Moczko, Richard Díaz, Bernabé Rivas, Camilo García, Eduardo Pereira, Sergey Piletsky, César Cáceres. Molecularly Imprinted Nanoparticles Assay (MINA) in Pseudo ELISA: An Alternative to Detect and Quantify Octopamine in Water and Human Urine Samples. Polymers 2019, 11 (9) , 1497. https://doi.org/10.3390/polym11091497
    89. Jingjing Zhou, Yufei Wang, Yong Ma, Baoliang Zhang, Qiuyu Zhang. Surface molecularly imprinted thermo-sensitive polymers based on light-weight hollow magnetic microspheres for specific recognition of BSA. Applied Surface Science 2019, 486 , 265-273. https://doi.org/10.1016/j.apsusc.2019.04.159
    90. Ewa Moczko, Antonio Guerreiro, César Cáceres, Elena Piletska, Börje Sellergren, Sergey A. Piletsky. Epitope approach in molecular imprinting of antibodies. Journal of Chromatography B 2019, 1124 , 1-6. https://doi.org/10.1016/j.jchromb.2019.05.024
    91. Joseph W. Lowdon, Kasper Eersels, Renato Rogosic, Tine Boonen, Benjamin Heidt, Hanne Diliën, Bart van Grinsven, Thomas J. Cleij. Surface grafted molecularly imprinted polymeric receptor layers for thermal detection of the New Psychoactive substance 2-methoxphenidine. Sensors and Actuators A: Physical 2019, 295 , 586-595. https://doi.org/10.1016/j.sna.2019.06.029
    92. Sihui Hong, Yongxin She, Xiaolin Cao, Miao Wang, Yahui He, Lufei Zheng, Shanshan Wang, A. M. Abd El-Aty, Ahmet Hacimüftüoglu, Mengmeng Yan, Jing Wang. A Novel CdSe/ZnS Quantum Dots Fluorescence Assay Based on Molecularly Imprinted Sensitive Membranes for Determination of Triazophos Residues in Cabbage and Apple. Frontiers in Chemistry 2019, 7 https://doi.org/10.3389/fchem.2019.00130
    93. Joseph W. Lowdon, Kasper Eersels, Renato Rogosic, Benjamin Heidt, Hanne Diliën, Erik Steen Redeker, Marloes Peeters, Bart van Grinsven, Thomas J. Cleij. Substrate displacement colorimetry for the detection of diarylethylamines. Sensors and Actuators B: Chemical 2019, 282 , 137-144. https://doi.org/10.1016/j.snb.2018.11.053
    94. Robert Crapnell, Alexander Hudson, Christopher Foster, Kasper Eersels, Bart Grinsven, Thomas Cleij, Craig Banks, Marloes Peeters. Recent Advances in Electrosynthesized Molecularly Imprinted Polymer Sensing Platforms for Bioanalyte Detection. Sensors 2019, 19 (5) , 1204. https://doi.org/10.3390/s19051204
    95. Muyasier Wubulikasimu, Turghun Muhammad, Mukhtar Imerhasan, Nurmemet Hudaberdi, Wenwu Yang, Jianzhang Zhao, Xiaojun Peng. Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers. RSC Advances 2019, 9 (12) , 6779-6784. https://doi.org/10.1039/C9RA00422J
    96. Faiza Jan Iftikhar, Afzal Shah, Mohammad Salim Akhter, Sevinc Kurbanoglu, Sibel A. Ozkan. Introduction to Nanosensors. 2019, 1-46. https://doi.org/10.1016/B978-0-12-816144-9.00001-8
    97. Sundas Sultan, Rafia Nimal, Saima Aftab, Sevinc Kurbanoglu, Afzal Shah, Sibel A. Ozkan. Photoelectrochemical Nanosensors. 2019, 197-229. https://doi.org/10.1016/B978-0-12-816144-9.00007-9
    98. Ximo Wang, Chen Chen, Longhua Xu, Hongyan Zhang, Zhixiang Xu. Development of molecularly imprinted biomimetic immunoassay method based on quantum dot marker for detection of phthalates. Food and Agricultural Immunology 2019, 30 (1) , 1007-1019. https://doi.org/10.1080/09540105.2019.1649371
    99. Muntazir S. Khan, Sourav Pal. Quantum mechanical studies on dioxin‐imprinted polymer precursor composites: Fundamental insights to enhance the binding strength and selectivity of biomarkers. Journal of Molecular Recognition 2018, 31 (11) https://doi.org/10.1002/jmr.2736
    100. Qiurui Liu, Jixin Tian, Mingdi Jiang, Xuguang Qiao, Zhixiang Xu. Direct Competitive Biomimetic Immunoassay Based on Quantum Dot Label for Simultaneous Determination of Two Pesticide Residues in Fruit and Vegetable Samples. Food Analytical Methods 2018, 11 (11) , 3015-3022. https://doi.org/10.1007/s12161-018-1285-z
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