Article

Adaptive Use of Bubble Wrap for Storing Liquid Samples and Performing Analytical Assays

Department of Chemistry & Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States
Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, Cambridge, Massachusetts 02138, United States
§ Kavli Institute for Bionano Science & Technology, Harvard University, 29 Oxford Street Cambridge, Massachusetts 02138, United States
Anal. Chem., 2014, 86 (15), pp 7478–7485
DOI: 10.1021/ac501206m
Publication Date (Web): July 1, 2014
Copyright © 2014 American Chemical Society

Abstract

Abstract Image

This paper demonstrates that the gas-filled compartments in the packing material commonly called “bubble wrap” can be repurposed in resource-limited regions as containers to store liquid samples, and to perform bioanalyses. The bubbles of bubble wrap are easily filled by injecting the samples into them using a syringe with a needle or a pipet tip, and then sealing the hole with nail hardener. The bubbles are transparent in the visible range of the spectrum, and can be used as “cuvettes” for absorbance and fluorescence measurements. The interiors of these bubbles are sterile and allow storage of samples without the need for expensive sterilization equipment. The bubbles are also permeable to gases, and can be used to culture and store micro-organisms. By incorporating carbon electrodes, these bubbles can be used as electrochemical cells. This paper demonstrates the capabilities of the bubbles by culturing E. coli, growing C. elegans, measuring glucose and hemoglobin spectrophotometrically, and measuring ferrocyanide electrochemically, all within the bubbles.

Additional information as noted in text (Figures S1−S3). This material is available free of charge via the Internet at http://pubs.acs.org.

Citation data is made available by participants in Crossref's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search inSciFinder.

Explore by:

Metrics

Article Views: 3,104 Times
Received 3 April 2014
Date accepted 1 July 2014
Published online 1 July 2014
Published in print 5 August 2014
+
Altmetric Logo Icon More Article Metrics

This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies. Read the ACS privacy policy.

CONTINUE