ACS Publications. Most Trusted. Most Cited. Most Read
Identification of Protein Remains in Archaeological Potsherds by Proteomics

OR SEARCH CITATIONS

My Activity
Publications

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2008, 80, 12, 4590-4597
    Accelerated Article

    Identification of Protein Remains in Archaeological Potsherds by Proteomics
    Click to copy article linkArticle link copied!

    View Author Information
    Chimie Organique et Macromoléculaire, UMR CNRS 8009, and Protéomique, Modifications Post-traductionnelles et Glycobiologie, IFR 147, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq Cedex, France, and Department of Anthropology, Smithsonian Institution, MRC 112, Washington, D.C. 20013-7012
    * To whom correspondence should be addressed. E-mail: [email protected]. Phone: 333 20 33 64 33 . Fax: 333 20 33 61 36.
    †Université des Sciences et Technologies de Lille.
    ‡Current address: BioArch, University of York, Biology (S Block), P.O. Box 373, York YO10 5YW, UK.
    §Smithsonian Institution.
    Other Access Options

    Analytical Chemistry

    Cite this: Anal. Chem. 2008, 80, 12, 4590–4597
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ac800515v
    Published May 22, 2008
    Copyright © 2008 American Chemical Society
    Request reuse permissions

    Abstract

    Click to copy section linkSection link copied!

    We demonstrate here the possibility of identifying proteins trapped in few milligrams of the clay matrix of a 1200−1400 AD Iñupiat potsherd fragment from Point Barrow, Alaska, by a dedicated proteomics approach. The four main steps of a proteomics analysis, (i) protein extraction from biological samples, (ii) protein hydrolysis using a hydrolase enzyme, (iii) nanoLC, nanoESI MS, and MS/MS analysis of the generated peptides, and (iv) protein identification using protein databank proceeded from genomic data, have been optimized for archeological remains. Briefly our procedure starts by grinding the potsherds, extraction with 1% trifluoroacetic acid, digestion with excess of trypsin, nanoLC, nanoESI FT-ICR analysis, and data mining by homology search. The developed conditions were evaluated on protein extracts from remains obtained by heated muscle tissues and blubbers of different seal and whale species, these samples representing the main diet sources of the Eskimo population. Most of the proteins were identified by sequence homology to other species due to the lack of cetacean and pinniped proteins in the databanks. More interestingly, two proteins, myoglobin and hemoglobin, respectively, identified in muscle tissue samples and blubber samples highlight several specific peptides of cetacean and pinniped species; these peptides are significant to prove the presence of these marine species in the analyzed samples. Based on the developed methodology and on protein identification results obtained from the heated seal/whale muscle tissues and blubbers, the analysis of the clay matrix of a 1200−1400 AD Iñupiat potsherd fragment from Point Barrow was investigated. The described method succeeds in identifying four peptides corresponding to the harbor seal myoglobin (species Phoca vitulina) with a measured mass accuracy better than 1 ppm (MS and MS/MS experiments) including one specific peptide of the cetacean and pinniped species and one specific peptide of the seal species. These results highlight, for the first time, a methodology able to identify proteins from a few milligrams of archeological potsherd buried for years; the obtained results confirm the presence of a seal muscle tissue protein in this Punuk potsherd.

    Copyright © 2008 American Chemical Society

    Subjects

    what are subjects

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai
    powered by  Scite

    This article is cited by 110 publications.

    1. Yuxin Huang, Zhijiang Wu, Hailiang Yang, Yanan Wang, Liping Bao, Yang Zhou, Huabing Wang. Rapid Identification of Ancient Leather Species Using an Enzyme-Linked Immunosorbent Assay Based on Proteomic and Evolutionary Analyses. Journal of Proteome Research 2024, 23 (12) , 5520-5530. https://doi.org/10.1021/acs.jproteome.4c00661
    2. Joshua Driscoll Jacob C. Damm . A First Draught: Pitfalls and Potentials in the Archaeological Chemistry of Beer. , 11-63. https://doi.org/10.1021/bk-2023-1446.ch002
    3. Manasij Pal Chowdhury, Cheryl Makarewicz, Henny Piezonka, Michael Buckley. Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications. Journal of Proteome Research 2022, 21 (11) , 2619-2634. https://doi.org/10.1021/acs.jproteome.2c00340
    4. Christina Warinner, Kristine Korzow Richter, Matthew J. Collins. Paleoproteomics. Chemical Reviews 2022, 122 (16) , 13401-13446. https://doi.org/10.1021/acs.chemrev.1c00703
    5. Hailing Zheng, Hailiang Yang, Yang Zhou, Tianxiao Li, Qinglin Ma, Bing Wang, Qin Fang, Haixiang Chen. Rapid Enrichment and Detection of Silk Residues from Tombs by Double-Antibody Sandwich ELISA Based on Immunomagnetic Beads. Analytical Chemistry 2021, 93 (43) , 14440-14447. https://doi.org/10.1021/acs.analchem.1c02556
    6. Cosima D. Calvano, Elena C.L. Rigante, Tommaso R.I. Cataldi, Luigia Sabbatini. In Situ Hydrogel Extraction with Dual-Enzyme Digestion of Proteinaceous Binders: the Key for Reliable Mass Spectrometry Investigations of Artworks. Analytical Chemistry 2020, 92 (15) , 10257-10261. https://doi.org/10.1021/acs.analchem.0c01898
    7. Marcello Manfredi, Elettra Barberis, Fabio Gosetti, Eleonora Conte, Giorgio Gatti, Clara Mattu, Elisa Robotti, Gleb Zilberstein, Igor Koman, Svetlana Zilberstein, Emilio Marengo, and Pier Giorgio Righetti . Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects. Analytical Chemistry 2017, 89 (6) , 3310-3317. https://doi.org/10.1021/acs.analchem.6b03722
    8. Sophie Dallongeville, Nicolas Garnier, Christian Rolando, and Caroline Tokarski . Proteins in Art, Archaeology, and Paleontology: From Detection to Identification. Chemical Reviews 2016, 116 (1) , 2-79. https://doi.org/10.1021/acs.chemrev.5b00037
    9. Sophie Dallongeville, Monika Koperska, Nicolas Garnier, Geneviève Reille-Taillefert, Christian Rolando, and Caroline Tokarski . Identification of Animal Glue Species in Artworks Using Proteomics: Application to a 18th Century Gilt Sample. Analytical Chemistry 2011, 83 (24) , 9431-9437. https://doi.org/10.1021/ac201978j
    10. Joannes Adrianus Antonius Dekker, Richard Hagan, Matthew Collins, Jessica Hendy. An unclean slate, discrepancies between food input and recovered protein signal from experimental foodcrusts. 2025https://doi.org/10.1101/2025.02.03.636240
    11. Miranda Evans. The Demodifier: a tool for screening modification-induced alternate peptide taxonomy in palaeoproteomics. 2025https://doi.org/10.1101/2025.01.09.632126
    12. Kalyan Sekhar Chakraborty, Lindsey Paskulin, Prabodh Shirvalkar, Yadubirsingh Rawat, Heather M.-L. Miller, Greg Slater, Camilla Speller. Assessing the applicability of protein residues in combination with lipid residues to reconstruct Indus foodways from Gujarat. Quaternary Environments and Humans 2024, 2 (6) , 100038. https://doi.org/10.1016/j.qeh.2024.100038
    13. Janez Kosel, Polonca Ropret. The potential of aptamers for the analysis of ceramic bound proteins found within pottery. Scientific Reports 2024, 14 (1) https://doi.org/10.1038/s41598-024-70048-8
    14. Liwei Tan, Xiaopan Fan, Huiyun Rao, Hui Zhang, Yimin Yang. The scientific analysis of the bronze mous excavated from Wushan, Chongqing, China: new perspectives from alloy composition analysis and proteomic method. Heritage Science 2024, 12 (1) https://doi.org/10.1186/s40494-024-01203-7
    15. Caroline Solazzo, William W. Fitzhugh. Proteomics identify bowhead whale muscle tissue and baleen in cinder residues at a 16th c. Basque whaling site. Journal of Archaeological Science: Reports 2024, 58 , 104691. https://doi.org/10.1016/j.jasrep.2024.104691
    16. Miranda Evans, Richard Hagan, Oliver J. Boyd, Manon Bondetti, Oliver E. Craig, Matthew J. Collins, Jessica Hendy. The impact of cooking and burial on proteins: a characterisation of experimental foodcrusts and ceramics. Royal Society Open Science 2024, 11 (9) https://doi.org/10.1098/rsos.240610
    17. Philippe Charlier, Virginie Bourdin, Didier N'Dah, Mélodie Kielbasa, Olivier Pible, Jean Armengaud. Metaproteomic analysis of King Ghezo tomb wall (Abomey, Benin) confirms 19th century voodoo sacrifices. PROTEOMICS 2024, 24 (16) https://doi.org/10.1002/pmic.202400048
    18. Lindsey Paskulin, Krista McGrath, Richard Hagan, Camilla Speller, Marian Berihuete-Azorín, Hans-Peter Stika, Soultana-Maria Valamoti, Jessica Hendy. Leave no stone unturned: Exploring the metaproteome of beerstone for the identification of archaeological beer production. Journal of Archaeological Science 2024, 168 , 106019. https://doi.org/10.1016/j.jas.2024.106019
    19. Shuxuan Shi, Ping Du, Liangsai Zhu, Feng Wang, Shuya Wei. Multi-analytical techniques used for characterization of the cosmetic residue in the Tang Dynasty. Journal of Archaeological Science: Reports 2024, 55 , 104463. https://doi.org/10.1016/j.jasrep.2024.104463
    20. Miranda Evans, Richard Hagan, Oliver Boyd, Manon Bondetti, Oliver Craig, Matthew Collins, Jessica Hendy. The impact of cooking and burial on proteins: a characterisation of experimental foodcrusts and ceramics. 2024https://doi.org/10.1101/2024.04.03.587902
    21. Marjolein Admiraal, Peter D. Jordan, Helen M. Talbot, Manon Bondetti, Alejandro Serna, Karine Taché, Matthew von Tersch, Jessica Hendy, Krista McGrath, Oliver E. Craig, Alexandre Lucquin. The role of salmon fishing in the adoption of pottery technology in subarctic Alaska. Journal of Archaeological Science 2023, 157 , 105824. https://doi.org/10.1016/j.jas.2023.105824
    22. Joannes Dekker, Tony Larson, Jordan Tzvetkov, Virginia L. Harvey, Adam Dowle, Richard Hagan, Paul Genever, Sarah Schrader, Marie Soressi, Jessica Hendy. Spatial analysis of the ancient proteome of archeological teeth using mass spectrometry imaging. Rapid Communications in Mass Spectrometry 2023, 37 (8) https://doi.org/10.1002/rcm.9486
    23. Jasmine Hertzog, Hitomi Fujii, Rugilė Žostautaitė, Agnès Lattuati-Derieux, Pascale Richardin, Vincent Carré, Frédéric Aubriet, Philippe Schmitt-Kopplin. Unravelling the Egyptian embalming materials by a multi-method approach comprising high-resolution mass spectrometry. Journal of Archaeological Science: Reports 2023, 48 , 103861. https://doi.org/10.1016/j.jasrep.2023.103861
    24. Patrick Leopold Rüther, Immanuel Mirnes Husic, Pernille Bangsgaard, Kristian Murphy Gregersen, Pernille Pantmann, Milena Carvalho, Ricardo Miguel Godinho, Lukas Friedl, João Cascalheira, Alberto John Taurozzi, Marie Louise Schjellerup Jørkov, Michael M. Benedetti, Jonathan Haws, Nuno Bicho, Frido Welker, Enrico Cappellini, Jesper Velgaard Olsen. SPIN enables high throughput species identification of archaeological bone by proteomics. Nature Communications 2022, 13 (1) https://doi.org/10.1038/s41467-022-30097-x
    25. Carli Peters, Kristine K. Richter, Jens-Christian Svenning, Nicole Boivin. Leveraging palaeoproteomics to address conservation and restoration agendas. iScience 2022, 25 (5) , 104195. https://doi.org/10.1016/j.isci.2022.104195
    26. Anne Charpentier, Ana S.L. Rodrigues, Claire Houmard, Alexandre Lefebvre, Krista McGrath, Camilla Speller, Laura van der Sluis, Antoine Zazzo, Jean-Marc Pétillon. What's in a whale bone? Combining new analytical methods, ecology and history to shed light on ancient human-whale interactions. Quaternary Science Reviews 2022, 284 , 107470. https://doi.org/10.1016/j.quascirev.2022.107470
    27. Manasij Pal Chowdhury, Michael Buckley. Trends in deamidation across archaeological bones, ceramics and dental calculus. Methods 2022, 200 , 67-79. https://doi.org/10.1016/j.ymeth.2021.08.004
    28. Dylan H. Multari, Prathiba Ravishankar, Geraldine J. Sullivan, Ronika K. Power, Constance Lord, James A. Fraser, Paul A. Haynes. Development of a novel minimally invasive sampling and analysis technique using skin sampling tape strips for bioarchaeological proteomics. Journal of Archaeological Science 2022, 139 , 105548. https://doi.org/10.1016/j.jas.2022.105548
    29. Michael W. Gregg. Organic Residues. 2022, 1-11. https://doi.org/10.1007/978-3-030-44600-0_36-1
    30. Peter Jordan, Irina Y. Ponkratova, Viktor M. Dyakonov, Elena N. Solovyova, Toshiro Yamahara, Hirofumi Kato, Marjolein Admiraal. Tracking the Adoption of Early Pottery Traditions into Maritime Northeast Asia: Emerging Insights and New Questions. 2022, 315-345. https://doi.org/10.1007/978-981-19-1118-7_14
    31. Jasmine Hertzog, Hitomi Fujii, Rugilė Žostautaitė, Agnès Lattuati-Derieux, Pascale Richardin, Vincent Carré, Frédéric Aubriet, Philippe Schmitt-Kopplin. Unravelling the Egyptian Embalming Materials by a Multi-Method Approach Comprising High-Resolution Mass Spectrometry. SSRN Electronic Journal 2022, 45 https://doi.org/10.2139/ssrn.4098910
    32. Nicolas Garnier. Chimie moléculaire en cuisine : quelle approche analytique pour quels résultats ? Proposition d’une méthode d’analyse-omique des résidus organiques dans des céramiques. Bulletin de Correspondance Hellénique 2022, 146.1 , 285-320. https://doi.org/10.4000/13g2y
    33. Gleb Zilberstein, Svetlana Zilberstein, Pier Giorgio Righetti. Richard the Lionheart and the Ferocious Saladin Face to Face in Arsuf: A Proteomic Study. Heritage 2021, 4 (4) , 3382-3401. https://doi.org/10.3390/heritage4040188
    34. Thomas Oldenburg, Melisa Brown, Jamie Inwood, Jagoš Radović, Ryan Snowdon, Steve Larter, Julio Mercader, . A novel route for identifying starch diagenetic products in the archaeological record. PLOS ONE 2021, 16 (11) , e0258779. https://doi.org/10.1371/journal.pone.0258779
    35. Manasij Pal Chowdhury, Stuart Campbell, Michael Buckley. Proteomic analysis of archaeological ceramics from Tell Khaiber, southern Iraq. Journal of Archaeological Science 2021, 132 , 105414. https://doi.org/10.1016/j.jas.2021.105414
    36. Jasmine Hertzog, Hitomi Fujii, Andrea Babbi, Agnès Lattuati-Derieux, Philippe Schmitt-Kopplin. Chemical composition overview on two organic residues from the inner part of an archaeological bronze vessel from Cumae (Italy) by GC–MS and FTICR MS analyses. The European Physical Journal Plus 2021, 136 (6) https://doi.org/10.1140/epjp/s13360-021-01627-1
    37. Ivana K. Levy, Ricardo Neme Tauil, Ana Rosso, Maria P. Valacco, Silvia Moreno, Fernando Guzmán, Gabriela Siracusano, Marta S. Maier. Finding of muscle proteins in art samples from mid-18th century murals by LC–MSMS. Journal of Cultural Heritage 2021, 48 , 227-235. https://doi.org/10.1016/j.culher.2020.11.005
    38. Davide Tanasi, Annamaria Cucina, Vincenzo Cunsolo, Rosaria Saletti, Antonella Di Francesco, Enrico Greco, Salvatore Foti. Paleoproteomic profiling of organic residues on prehistoric pottery from Malta. Amino Acids 2021, 53 (2) , 295-312. https://doi.org/10.1007/s00726-021-02946-4
    39. Jessica Hendy. Ancient protein analysis in archaeology. Science Advances 2021, 7 (3) https://doi.org/10.1126/sciadv.abb9314
    40. E. Ribechini. Organic Residues from Archaeological Findings. 2021, 283-296. https://doi.org/10.1039/9781788015974-00283
    41. Amra Aksamija, Stéphanie Devassine, Fabrice Bray, Marc Bormand, Anne Bouquillon, Christian Rolando, Caroline Tokarski. Proteomic analysis of Renaissance stucco: development of the analytical protocol and first results. Technè 2021, 51 , 75-83. https://doi.org/10.4000/techne.8990
    42. . Ancient Protein Sequences. 2020, 113-126. https://doi.org/10.1002/9781119089537.ch5
    43. Marjolein Admiraal, Alexandre Lucquin, Matthew von Tersch, Oliver E. Craig, Peter D. Jordan. The adoption of pottery on Kodiak Island: Insights from organic residue analysis. Quaternary International 2020, 554 , 128-142. https://doi.org/10.1016/j.quaint.2020.06.024
    44. Benjamin Irvine, Kameray Özdemir. Biogeochemical Approaches to Bioarchaeological Research in Turkey: A Review. Journal of Eastern Mediterranean Archaeology and Heritage Studies 2020, 8 (2) , 174-199. https://doi.org/10.5325/jeasmedarcherstu.8.2.0174
    45. Alan A. Doucette, Jessica L. Nickerson. Developing front‐end devices for improved sample preparation in MS‐based proteome analysis. Journal of Mass Spectrometry 2020, 55 (3) https://doi.org/10.1002/jms.4494
    46. Rikai Sawafuji, Takumi Tsutaya. Applications of mass spectrometry-based proteomics in archaeology and palaeoanthropology. Anthropological Science (Japanese Series) 2020, 128 (1) , 1-19. https://doi.org/10.1537/asj.200213
    47. Jessica Hendy, Nienke van Doorn, Matthew Collins. Proteomics. 2019, 35-69. https://doi.org/10.1017/9781139013826.003
    48. Oliver E. Craig, Hayley Saul, Cynthianne Spiteri. Residue Analysis. 2019, 70-98. https://doi.org/10.1017/9781139013826.004
    49. Jincui Gu, Qingqing Li, Boyi Chen, Chengfeng Xu, Hailing Zheng, Yang Zhou, Zhiqin Peng, Zhiwen Hu, Bing Wang. Species identification of Bombyx mori and Antheraea pernyi silk via immunology and proteomics. Scientific Reports 2019, 9 (1) https://doi.org/10.1038/s41598-019-45698-8
    50. Fabiana Di Gianvincenzo, Clara Granzotto, Enrico Cappellini. Skin, Furs, and Textiles: Mass Spectrometry-based Analysis of Ancient Protein Residues. 2019, 304-316. https://doi.org/10.1017/9781108656405.013
    51. Tang Min, Yan Jian-Ye, Ren Jie, Li Shun-Xiang. Differentiating Trachemys scripta elegans Shell Glue from Chinemys reevesii Shell Glue by UPLC-QTOF/MS Coupled with Binary Compare Tool of UNIFI. Digital Chinese Medicine 2019, 2 (1) , 50-58. https://doi.org/10.1016/j.dcmed.2019.05.006
    52. Valentine Roux. Classification of Archaeological Assemblages According to the Chaîne opératoire Concept: Functional and Sociological Characterization. 2019, 217-258. https://doi.org/10.1007/978-3-030-03973-8_4
    53. Roberto Vinciguerra, Anna Illiano, Addolorata De Chiaro, Andrea Carpentieri, Anna Lluveras-Tenorio, Ilaria Bonaduce, Gennaro Marino, Piero Pucci, Angela Amoresano, Leila Birolo. Identification of proteinaceous binders in paintings: A targeted proteomic approach for cultural heritage. Microchemical Journal 2019, 144 , 319-328. https://doi.org/10.1016/j.microc.2018.09.021
    54. Nicolas Garnier, Dario Bernal-Casasola, Cyril Driard, Inês Vaz Pinto. Looking for Ancient Fish Products Through Invisible Biomolecular Residues in the Roman Production Vats from the Atlantic Coast. Journal of Maritime Archaeology 2018, 13 (3) , 285-328. https://doi.org/10.1007/s11457-018-9219-x
    55. Ivana K. Levy, Ricardo Neme Tauil, Maria P. Valacco, Silvia Moreno, Gabriela Siracusano, Marta S. Maier. Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). Microchemical Journal 2018, 143 , 457-466. https://doi.org/10.1016/j.microc.2018.07.030
    56. Jessica Hendy, Andre C. Colonese, Ingmar Franz, Ricardo Fernandes, Roman Fischer, David Orton, Alexandre Lucquin, Luke Spindler, Jana Anvari, Elizabeth Stroud, Peter F. Biehl, Camilla Speller, Nicole Boivin, Meaghan Mackie, Rosa R. Jersie-Christensen, Jesper V. Olsen, Matthew J. Collins, Oliver E. Craig, Eva Rosenstock. Ancient proteins from ceramic vessels at Çatalhöyük West reveal the hidden cuisine of early farmers. Nature Communications 2018, 9 (1) https://doi.org/10.1038/s41467-018-06335-6
    57. Andrew Lewis Barker, Steve Wolverton. Mass Spectrometry-Based Identification of Ceramic-Bound Archaeological Protein Residues: Method Validation, Residue Taphonomy, and Prospects. 2018https://doi.org/10.12794/metadc1404522
    58. Marjolein Admiraal, Rick Knecht. Understanding the Function of Container Technologies in Prehistoric Southwest Alaska. 2018, 104-127. https://doi.org/10.1017/9781316339374.006
    59. Shelby L. Anderson. Ethnographic and Archaeological Perspectives on the Use Life of Northwest Alaskan Pottery. 2018, 128-151. https://doi.org/10.1017/9781316339374.007
    60. Sibilla Orsini, Emilia Bramanti, Ilaria Bonaduce. Analytical pyrolysis to gain insights into the protein structure. The case of ovalbumin. Journal of Analytical and Applied Pyrolysis 2018, 133 , 59-67. https://doi.org/10.1016/j.jaap.2018.04.020
    61. Jessica Hendy, Christina Warinner, Abigail Bouwman, Matthew J. Collins, Sarah Fiddyment, Roman Fischer, Richard Hagan, Courtney A. Hofman, Malin Holst, Eros Chaves, Lauren Klaus, Greger Larson, Meaghan Mackie, Krista McGrath, Amy Z. Mundorff, Anita Radini, Huiyun Rao, Christian Trachsel, Irina M. Velsko, Camilla F. Speller. Proteomic evidence of dietary sources in ancient dental calculus. Proceedings of the Royal Society B: Biological Sciences 2018, 285 (1883) , 20180977. https://doi.org/10.1098/rspb.2018.0977
    62. Maria Gabriella Giuffrida, Roberto Mazzoli, Enrica Pessione. Back to the past: deciphering cultural heritage secrets by protein identification. Applied Microbiology and Biotechnology 2018, 102 (13) , 5445-5455. https://doi.org/10.1007/s00253-018-8963-z
    63. Andrew Barker, Jonathan Dombrosky, Barney Venables, Steve Wolverton. Taphonomy and negative results: An integrated approach to ceramic-bound protein residue analysis. Journal of Archaeological Science 2018, 94 , 32-43. https://doi.org/10.1016/j.jas.2018.03.004
    64. Jessica Hendy, Frido Welker, Beatrice Demarchi, Camilla Speller, Christina Warinner, Matthew J. Collins. A guide to ancient protein studies. Nature Ecology & Evolution 2018, 2 (5) , 791-799. https://doi.org/10.1038/s41559-018-0510-x
    65. Michael Buckley. Paleoproteomics: An Introduction to the Analysis of Ancient Proteins by Soft Ionisation Mass Spectrometry. 2018, 31-52. https://doi.org/10.1007/13836_2018_50
    66. HouYuan Lu. New methods and progress in research on the origins and evolution of prehistoric agriculture in China. Science China Earth Sciences 2017, 60 (12) , 2141-2159. https://doi.org/10.1007/s11430-017-9145-2
    67. Andre Carlo Colonese, Jessica Hendy, Alexandre Lucquin, Camilla F. Speller, Matthew J. Collins, Francesco Carrer, Regula Gubler, Marlu Kühn, Roman Fischer, Oliver E. Craig. New criteria for the molecular identification of cereal grains associated with archaeological artefacts. Scientific Reports 2017, 7 (1) https://doi.org/10.1038/s41598-017-06390-x
    68. Philippe Charlier, Anaïs Augias, Philippe Sansonetti, Céline Bon, Sean Kennedy, Laure Segurel. Fæces vivos docent. médecine/sciences 2017, 33 (11) , 984-990. https://doi.org/10.1051/medsci/20173311016
    69. Ashley Scott, Barney J. Venables. Development of a Targeted Protein Residue Analysis Approach in Archaeology. 2017https://doi.org/10.12794/metadc1011863
    70. Shelby L. Anderson, Shannon Tushingham, Tammy Y. Buonasera. AQUATIC ADAPTATIONS AND THE ADOPTION OF ARCTIC POTTERY TECHNOLOGY: RESULTS OF RESIDUE ANALYSIS. American Antiquity 2017, 82 (3) , 452-479. https://doi.org/10.1017/aaq.2017.8
    71. Vijay Sathe. Smaller but not Secondary: Evidence of Rodents in Archaeological Context in India. Ancient Asia 2017, 8 https://doi.org/10.5334/aa.131
    72. Jean‐François Bernier, Najat Bhiry, Daniel Gendron. Butchering Site Evolution Induced by Past and Recent Snowmelt Runoff: The Saunitarlik Site (JiEv‐15), Aivirtuuq Peninsula, Nunavik, Canada. Geoarchaeology 2017, 32 (3) , 343-365. https://doi.org/10.1002/gea.21607
    73. Xiang Yu, Yuxuan Gong, Panpan Tan, Jiaping Shu, Feng Liu, Junchang Yang, Decai Gong. Mass spectrometry analysis of textile used in decorating the coronet of Empress Xiao of the Sui Dynasty (581–618 A.D.). Journal of Cultural Heritage 2017, 25 , 185-188. https://doi.org/10.1016/j.culher.2017.01.003
    74. Sibilla Orsini, Federica Parlanti, Ilaria Bonaduce. Analytical pyrolysis of proteins in samples from artistic and archaeological objects. Journal of Analytical and Applied Pyrolysis 2017, 124 , 643-657. https://doi.org/10.1016/j.jaap.2016.12.017
    75. Anna Shevchenko, Yimin Yang, Andrea Knaust, Jean-Marc Verbavatz, Huijuan Mai, Bo Wang, Changsui Wang, Andrej Shevchenko, . Open sesame: Identification of sesame oil and oil soot ink in organic deposits of Tang Dynasty lamps from Astana necropolis in China. PLOS ONE 2017, 12 (2) , e0158636. https://doi.org/10.1371/journal.pone.0158636
    76. Conner J. Wiktorowicz, Bettina Arnold, John E. Wiktorowicz, Matthew L. Murray, Alexander Kurosky. Hemorrhagic fever virus, human blood, and tissues in Iron Age mortuary vessels. Journal of Archaeological Science 2017, 78 , 29-39. https://doi.org/10.1016/j.jas.2016.11.009
    77. Michael W. Gregg. Organic Residues. 2017, 555-566. https://doi.org/10.1007/978-1-4020-4409-0_36
    78. Niels Bleicher, Christian Kelstrup, Jesper V. Olsen, Enrico Cappellini. Molecular evidence of use of hide glue in 4th millennium BC Europe. Journal of Archaeological Science 2015, 63 , 65-71. https://doi.org/10.1016/j.jas.2015.08.012
    79. Huiyun Rao, Yimin Yang, Idelisi Abuduresule, Wenying Li, Xingjun Hu, Changsui Wang. Proteomic identification of adhesive on a bone sculpture-inlaid wooden artifact from the Xiaohe Cemetery, Xinjiang, China. Journal of Archaeological Science 2015, 53 , 148-155. https://doi.org/10.1016/j.jas.2014.10.010
    80. Huiyun Rao, Bo Li, Yimin Yang, Qinglin Ma, Changsui Wang. Proteomic identification of organic additives in the mortars of ancient Chinese wooden buildings. Analytical Methods 2015, 7 (1) , 143-149. https://doi.org/10.1039/C4AY01766H
    81. Carl Heron, Oliver E Craig. Aquatic Resources in Foodcrusts: Identification and Implication. Radiocarbon 2015, 57 (4) , 707-719. https://doi.org/10.2458/azu_rc.57.18454
    82. Zhanyun Zhu, Hua‐feng Chen, Li Li, De‐cai Gong, Xiang Gao, Junchang Yang, Xichen Zhao, Kunzhang Ji. Biomass Spectrometry Identification of the Fibre Material in the Pall Imprint Excavated from Grave M1 , Peng‐state Cemetery, S hanxi, C hina. Archaeometry 2014, 56 (4) , 681-688. https://doi.org/10.1111/arcm.12029
    83. Anna Shevchenko, Yimin Yang, Andrea Knaust, Henrik Thomas, Hongen Jiang, Enguo Lu, Changsui Wang, Andrej Shevchenko. Proteomics identifies the composition and manufacturing recipe of the 2500-year old sourdough bread from Subeixi cemetery in China. Journal of Proteomics 2014, 105 , 363-371. https://doi.org/10.1016/j.jprot.2013.11.016
    84. Yimin Yang, Anna Shevchenko, Andrea Knaust, Idelisi Abuduresule, Wenying Li, Xingjun Hu, Changsui Wang, Andrej Shevchenko. Proteomics evidence for kefir dairy in Early Bronze Age China. Journal of Archaeological Science 2014, 45 , 178-186. https://doi.org/10.1016/j.jas.2014.02.005
    85. L. Cramp, R.P. Evershed. Reconstructing Aquatic Resource Exploitation in Human Prehistory Using Lipid Biomarkers and Stable Isotopes. 2014, 319-339. https://doi.org/10.1016/B978-0-08-095975-7.01225-0
    86. Xian-Long Cheng, Feng Wei, Jia Chen, Ming-hua Li, Lei Zhang, Ying-Yong Zhao, Xin-Yue Xiao, Shuang-cheng Ma, Rui-Chao Lin. Using the Doubly Charged Selected Ion Coupled with MS/MS Fragments Monitoring (DCSI-MS/MS) Mode for the Identification of Gelatin Species. Journal of Analytical Methods in Chemistry 2014, 2014 , 1-7. https://doi.org/10.1155/2014/764397
    87. Nicolas Garnier. Analyse chimique des sauces et des conserves de poissons : un état de la question. 2014, 17-35. https://doi.org/10.4000/books.pccj.1626
    88. Frank Maixner, Thorsten Overath, Dennis Linke, Marek Janko, Gea Guerriero, Bart H. J. van den Berg, Bjoern Stade, Petra Leidinger, Christina Backes, Marta Jaremek, Benny Kneissl, Benjamin Meder, Andre Franke, Eduard Egarter-Vigl, Eckart Meese, Andreas Schwarz, Andreas Tholey, Albert Zink, Andreas Keller. Paleoproteomic study of the Iceman’s brain tissue. Cellular and Molecular Life Sciences 2013, 70 (19) , 3709-3722. https://doi.org/10.1007/s00018-013-1360-y
    89. T. Tripković, C. Charvy, S. Alves, A.Đ. Lolić, R.M. Baošić, S.D. Nikolić-Mandić, J.C. Tabet. Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry. Talanta 2013, 113 , 49-61. https://doi.org/10.1016/j.talanta.2013.03.071
    90. Inez D. van der Werf, Cosima D. Calvano, Rocco Laviano, Antonella Simonetti, Luigia Sabbatini. Multi-technique chemical characterisation of a 12–13th-century painted Crucifix. Microchemical Journal 2013, 106 , 87-94. https://doi.org/10.1016/j.microc.2012.05.011
    91. Sophie Dallongeville, Mark Richter, Stephan Schäfer, Michael Kühlenthal, Nicolas Garnier, Christian Rolando, Caroline Tokarski. Proteomics applied to the authentication of fish glue: application to a 17th century artwork sample. The Analyst 2013, 138 (18) , 5357. https://doi.org/10.1039/c3an00786c
    92. Daniel P. Kirby, Michael Buckley, Ellen Promise, Sunia A. Trauger, T. Rose Holdcraft. Identification of collagen-based materials in cultural heritage. The Analyst 2013, 138 (17) , 4849. https://doi.org/10.1039/c3an00925d
    93. Steven Saverwyns*, Ina Vanden Berghe. Separation Techniques in Archaeometry. 2012, 132-162. https://doi.org/10.1039/9781849732741-00132
    94. Andrew Barker, Barney Venables, Stanley M. Stevens, Kent W Seeley, Peggy Wang, Steve Wolverton. An Optimized Approach for Protein Residue Extraction and Identification from Ceramics After Cooking. Journal of Archaeological Method and Theory 2012, 19 (3) , 407-439. https://doi.org/10.1007/s10816-011-9120-5
    95. Chuan Hong, Hongen Jiang, Enguo Lü, Yunfei Wu, Lihai Guo, Yongming Xie, Changsui Wang, Yimin Yang, . Identification of Milk Component in Ancient Food Residue by Proteomics. PLoS ONE 2012, 7 (5) , e37053. https://doi.org/10.1371/journal.pone.0037053
    96. Wim Fremout, Maarten Dhaenens, Steven Saverwyns, Jana Sanyova, Peter Vandenabeele, Dieter Deforce, Luc Moens. Development of a dedicated peptide tandem mass spectral library for conservation science. Analytica Chimica Acta 2012, 728 , 39-48. https://doi.org/10.1016/j.aca.2012.03.037
    97. Maria Perla Colombini, Francesca Modugno, Erika Ribechini. Archaeometric Data from Mass Spectrometric Analysis of Organic Materials: Proteins, Lipids, Terpenoid Resins, Lignocellulosic Polymers, and Dyestuff. 2012, 797-828. https://doi.org/10.1002/9781118180730.ch36
    98. Inez D. van der Werf, Cosima D. Calvano, Francesco Palmisano, Luigia Sabbatini. A simple protocol for Matrix Assisted Laser Desorption Ionization- time of flight-mass spectrometry (MALDI-TOF-MS) analysis of lipids and proteins in single microsamples of paintings. Analytica Chimica Acta 2012, 718 , 1-10. https://doi.org/10.1016/j.aca.2011.12.056
    99. Bartłomiej Witkowski, Magdalena Biesaga, Tomasz Gierczak. Proteinaceous binders identification in the works of art using ion-pairing free reversed-phase liquid chromatography coupled with tandem mass spectrometry. Analytical Methods 2012, 4 (5) , 1221. https://doi.org/10.1039/c2ay05605d
    100. Erika Ribechini, Francesca Modugno, Josefina Pérez-Arantegui, Maria Perla Colombini. Discovering the composition of ancient cosmetics and remedies: analytical techniques and materials. Analytical and Bioanalytical Chemistry 2011, 401 (6) , 1727-1738. https://doi.org/10.1007/s00216-011-5112-2
    Load all citations
    Download PDF
    Go to Analytical Chemistry
    Get e-Alerts
    Get e-Alerts

    Analytical Chemistry

    Cite this: Anal. Chem. 2008, 80, 12, 4590–4597
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ac800515v
    Published May 22, 2008
    Copyright © 2008 American Chemical Society
    Request reuse permissions

    Article Views

    1849

    Altmetric

    -

    Citations

    110
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.