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Quantitative Profiling Method for Oxylipin Metabolome by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

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    Quantitative Profiling Method for Oxylipin Metabolome by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry
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    Department of Entomology and Cancer Center, University of California, One Shields Avenue, Davis, California 95616
    * Corresponding author. Tel: +1 530 752 8465. Fax: +1 530 752 1537. E-mail: [email protected]
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    Analytical Chemistry

    Cite this: Anal. Chem. 2009, 81, 19, 8085–8093
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    https://doi.org/10.1021/ac901282n
    Published August 28, 2009
    Copyright © 2009 American Chemical Society
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    Abstract

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    Cyclooxygenase, lipoxygenase, and epoxygenase derived oxylipins, especially eicosanoids, play important roles in many physiological processes. Assessment of oxidized fatty acid levels is important for understanding their homeostatic and pathophysiological roles. Most reported methods examine these pathways in isolation. The work described here employed a solid phase extraction-liquid chromatography-electrospray ionization MS/MS (SPE-LC-ESI MS/MS) method to monitor these metabolites. In 21 min, 39 oxylipins were quantified along with eight corresponding internal standards. The limits of quantification were between 0.07 and 32 pg (20 pM−10 nM). Finally, the validated method was used to evaluate oxylipin profiles in lipopolysaccharide-exposed mice, an established septic inflammatory model. The method described here offers a useful tool for the evaluation of complex regulatory oxylipin responses in in vitro or in vivo studies.

    Copyright © 2009 American Chemical Society

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    Analytical Chemistry

    Cite this: Anal. Chem. 2009, 81, 19, 8085–8093
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    https://doi.org/10.1021/ac901282n
    Published August 28, 2009
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