Universal Sample Preparation Unlocking Multimodal Molecular Tissue ImagingClick to copy article linkArticle link copied!
- Andreas DannhornAndreas DannhornDepartment of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Andreas Dannhorn
- Emine KazancEmine KazancDepartment of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.More by Emine Kazanc
- Stephanie LingStephanie LingImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Stephanie Ling
- Chelsea NikulaChelsea NikulaNational Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.More by Chelsea Nikula
- Evdoxia Karali
- Maria Paola SerraMaria Paola SerraImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Maria Paola Serra
- Jean-Luc VorngJean-Luc VorngNational Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.More by Jean-Luc Vorng
- Paolo InglesePaolo IngleseDepartment of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.More by Paolo Inglese
- Gareth MaglennonGareth MaglennonOncology Safety, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Gareth Maglennon
- Gregory HammGregory HammImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Gregory Hamm
- John SwalesJohn SwalesImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by John Swales
- Nicole StrittmatterNicole StrittmatterImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Nicole Strittmatter
- Simon T. BarrySimon T. BarryBioscience, Discovery, Oncology R&D, AstraZeneca, Cambridge, United KingdomMore by Simon T. Barry
- Owen J. SansomOwen J. SansomCancer Research UK Beatson Institute, Glasgow, G61 1BD, United KingdomMore by Owen J. Sansom
- George PoulogiannisGeorge PoulogiannisThe Institute for Cancer Research (ICR), London SW7 3RP, U.K.More by George Poulogiannis
- Josephine BunchJosephine BunchNational Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.More by Josephine Bunch
- Richard JA GoodwinRichard JA GoodwinImaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.More by Richard JA Goodwin
- Zoltan Takats*Zoltan Takats*Phone: +44 (0)207 5942760. Email: [email protected]Department of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.More by Zoltan Takats
Abstract
A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) and sectioning using a cryomicrotome. The hydrogel was expected not to interfere with standard tissue characterization methods, histologically or analytically. We assessed the compatibility of this protocol with various mass spectrometric imaging methods including matrix-assisted laser desorption ionization (MALDI), desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS). We also demonstrated the suitability of the universal protocol for extraction based molecular biology techniques such as rt-PCR. The integration of multiple analytical modalities through this universal sample preparation protocol offers the ability to study tissues at a systems biology level and directly linking results to tissue morphology and cellular phenotype.
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