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ACS Publications. Most Trusted. Most Cited. Most Read
Universal Sample Preparation Unlocking Multimodal Molecular Tissue Imaging
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    Universal Sample Preparation Unlocking Multimodal Molecular Tissue Imaging
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    • Andreas Dannhorn
      Andreas Dannhorn
      Department of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Emine Kazanc
      Emine Kazanc
      Department of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.
      More by Emine Kazanc
    • Stephanie Ling
      Stephanie Ling
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Chelsea Nikula
      Chelsea Nikula
      National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.
    • Evdoxia Karali
      Evdoxia Karali
      The Institute for Cancer Research (ICR), London SW7 3RP, U.K.
    • Maria Paola Serra
      Maria Paola Serra
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Jean-Luc Vorng
      Jean-Luc Vorng
      National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.
    • Paolo Inglese
      Paolo Inglese
      Department of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.
    • Gareth Maglennon
      Gareth Maglennon
      Oncology Safety, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Gregory Hamm
      Gregory Hamm
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
      More by Gregory Hamm
    • John Swales
      John Swales
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
      More by John Swales
    • Nicole Strittmatter
      Nicole Strittmatter
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Simon T. Barry
      Simon T. Barry
      Bioscience, Discovery, Oncology R&D, AstraZeneca, Cambridge, United Kingdom
    • Owen J. Sansom
      Owen J. Sansom
      Cancer Research UK Beatson Institute, Glasgow, G61 1BD, United Kingdom
    • George Poulogiannis
      George Poulogiannis
      The Institute for Cancer Research (ICR), London SW7 3RP, U.K.
    • Josephine Bunch
      Josephine Bunch
      National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.
    • Richard JA Goodwin
      Richard JA Goodwin
      Imaging and Data analytics, Clinical Pharmacology and Safety Sciences (CPSS), AstraZeneca, Cambridge, U.K.
    • Zoltan Takats*
      Zoltan Takats
      Department of Digestion, Metabolism and Reproduction, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, U.K.
      *Phone: +44 (0)207 5942760. Email: [email protected]
    Other Access OptionsSupporting Information (1)

    Analytical Chemistry

    Cite this: Anal. Chem. 2020, 92, 16, 11080–11088
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    https://doi.org/10.1021/acs.analchem.0c00826
    Published June 10, 2020
    Copyright © 2020 American Chemical Society

    Abstract

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    A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) and sectioning using a cryomicrotome. The hydrogel was expected not to interfere with standard tissue characterization methods, histologically or analytically. We assessed the compatibility of this protocol with various mass spectrometric imaging methods including matrix-assisted laser desorption ionization (MALDI), desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS). We also demonstrated the suitability of the universal protocol for extraction based molecular biology techniques such as rt-PCR. The integration of multiple analytical modalities through this universal sample preparation protocol offers the ability to study tissues at a systems biology level and directly linking results to tissue morphology and cellular phenotype.

    Copyright © 2020 American Chemical Society

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.0c00826.

    • Additional information including Tables S1 and S2 and Figures S1–S7 (PDF)

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    Cited By

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    This article is cited by 66 publications.

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    Analytical Chemistry

    Cite this: Anal. Chem. 2020, 92, 16, 11080–11088
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    https://doi.org/10.1021/acs.analchem.0c00826
    Published June 10, 2020
    Copyright © 2020 American Chemical Society

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