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    Peptide Nucleic Acid-Functionalized Nanochannel Biosensor for the Highly Sensitive Detection of Tumor Exosomal MicroRNA
    Click to copy article linkArticle link copied!

    • Ping-Ping Xiao
      Ping-Ping Xiao
      School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      More by Ping-Ping Xiao
    • Qiang-Qiang Wan
      Qiang-Qiang Wan
      School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      Wuhan First Hospital, Wuhan 430022, China
      More by Qiang-Qiang Wan
    • Tangbin Liao
      Tangbin Liao
      School of Pharmacy, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      More by Tangbin Liao
    • Ji-Yuan Tu
      Ji-Yuan Tu
      School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      More by Ji-Yuan Tu
    • Guo-Jun Zhang*
      Guo-Jun Zhang
      School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      *Email: [email protected]
      More by Guo-Jun Zhang
    • Zhong-Yue Sun*
      Zhong-Yue Sun
      School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China
      *Email: [email protected]
      More by Zhong-Yue Sun
      Orcidhttps://orcid.org/0000-0001-9032-5607
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    Analytical Chemistry

    Cite this: Anal. Chem. 2021, 93, 31, 10966–10973
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    https://doi.org/10.1021/acs.analchem.1c01898
    Published July 30, 2021
    Copyright © 2021 American Chemical Society
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    Abstract

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    Compared with free miRNAs in blood, miRNAs in exosomes have higher abundance and stability. Therefore, miRNAs in exosomes can be regarded as an ideal tumor marker for early cancer diagnosis. Here, a peptide nucleic acid (PNA)-functionalized nanochannel biosensor for the ultrasensitive and specific detection of tumor exosomal miRNAs is proposed. After PNA was covalently bound to the inner surface of the nanochannels, the detection of tumor exosomal miRNAs was achieved by the charge changes on the surface of nanochannels before and after hybridization (PNA–miRNA). Due to the neutral characteristics of PNA, the efficiency of PNA–miRNA hybridization was improved by significantly reducing the background signal. This biosensor could not only specifically distinguish target miRNA-10b from single-base mismatched miRNA but also achieve a detection limit as low as 75 aM. Moreover, the biosensor was further used to detect exosomal miRNA-10b derived from pancreatic cancer cells and normal pancreatic cells. The results indicate that this biosensor could effectively distinguish pancreatic cancer tumor-derived exosomes from the normal control group, and the detection results show good consistency with those of the quantitative reverse-transcription polymerase chain reaction method. In addition, the biosensor was used to detect exosomal miRNA-10b in clinical plasma samples, and it was found that the content of exosomal miRNA-10b in cancer patients was generally higher than that of healthy individuals, proving that the method is expected to be applied for the early diagnosis of cancer.

    Copyright © 2021 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.1c01898.

    • Characterization of exosomes, qRT-PCR protocol, PNA probe sequence and miRNA sequences, comparison of different methods for exosomal miRNA detection, FE-SEM images of conical PET nanochannels, wettability of nanochannel before and after PNA modification, optimizations of experimental conditions, stability of the PNA-functionalized nanochannel biosensor, and TEM characterizations and NTA results of H6C7 exosomes (PDF)

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    This article is cited by 43 publications.

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    Analytical Chemistry

    Cite this: Anal. Chem. 2021, 93, 31, 10966–10973
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.1c01898
    Published July 30, 2021
    Copyright © 2021 American Chemical Society
    Request reuse permissions

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