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Protein Chips Compatible with MALDI Mass Spectrometry Prepared by Ambient Ion Landing

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Institute of Microbiology, v.v.i., Czech Academy of Sciences, CZ-142 20 Prague, Czech Republic
Faculty of Science, Charles University in Prague, CZ-128 00 Prague, Czech Republic
Faculty of Science, J. E. Purkyně University in Ustí nad Labem, CZ-400 96 Ústí nad Labem, Czech Republic
# AffiPro, s.r.o., CZ-250 63 Mratin, Czech Republic
§ BioVendor, a.s., CZ-621 00 Brno, Czech Republic
*E-mail: [email protected]. Tel.: +420 325 873 610.
*E-mail: [email protected]. Tel.: +420-734-492-970.
Cite this: Anal. Chem. 2016, 88, 17, 8526–8534
Publication Date (Web):August 1, 2016
https://doi.org/10.1021/acs.analchem.6b01366
Copyright © 2016 American Chemical Society
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Abstract

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We present a technology that allows the preparation of matrix-assisted laser desorption/ionization (MALDI)-compatible protein chips by ambient ion landing of proteins and successive utilization of the resulting protein chips for the development of bioanalytical assays. These assays are based on the interaction between the immobilized protein and the sampled analyte directly on the protein chip and subsequent in situ analysis by MALDI mass spectrometry. The electrosprayed proteins are immobilized on dry metal and metal oxide surfaces, which are nonreactive under normal conditions. The ion landing of electrosprayed protein molecules is performed under atmospheric pressure by an automated ion landing apparatus that can manufacture protein chips with a predefined array of sample positions or any other geometry of choice. The protein chips prepared by this technique are fully compatible with MALDI ionization because the metal-based substrates are conductive and durable enough to be used directly as MALDI plates. Compared to other materials, the nonreactive surfaces show minimal nonspecific interactions with chemical species in the investigated sample and are thus an ideal substrate for selective protein chips. Three types of protein chips were used in this report to demonstrate the bioanalytical applications of ambient ion landing. The protein chips with immobilized proteolytic enzymes showed the usefulness for fast in situ peptide MALDI sequencing; the lectin-based protein chips showed the ability to enrich glycopeptides from complex mixtures with subsequent MALDI analysis, and the protein chips with immobilized antibodies were used for a novel immunoMALDI workflow that allowed the enrichment of antigens from the serum followed by highly specific MALDI detection.

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The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.6b01366.

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Cited By

This article is cited by 13 publications.

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  2. Jana Fialova, Jaroslav Hrabak, Vendula Studentova, Daniel Kavan, Petr Pompach, Petr Novak. Three-Dimensional Printed Target Plates for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Analytical Chemistry 2020, 92 (19) , 12783-12788. https://doi.org/10.1021/acs.analchem.0c01737
  3. Huiyan Li, Robert Popp, Michael Chen, Elizabeth M. MacNamara, David Juncker, and Christoph H. Borchers . Bead-Extractor Assisted Ready-to-Use Reagent System (BEARS) for Immunoprecipitation Coupled to MALDI-MS. Analytical Chemistry 2017, 89 (7) , 3834-3839. https://doi.org/10.1021/acs.analchem.6b04169
  4. Chiara Salvitti, Anna Troiani, Franco Mazzei, Cristine D'Agostino, Rosaceleste Zumpano, Chiara Baldacchini, Anna Rita Bizzarri, Alessandra Tata, Federico Pepi. The use of a commercial ESI Z-spray source for ambient ion soft landing and microdroplet reactivity experiments. International Journal of Mass Spectrometry 2021, 468 , 116658. https://doi.org/10.1016/j.ijms.2021.116658
  5. Gargi S. Jagdale, Myung-Hoon Choi, Natasha P. Siepser, Soojin Jeong, Yi Wang, Rebecca X. Skalla, Kaixiang Huang, Xingchen Ye, Lane A. Baker. Electrospray deposition for single nanoparticle studies. Analytical Methods 2021, 13 (36) , 4105-4113. https://doi.org/10.1039/D1AY01295A
  6. Subhayu Nayek, Roberto Aguilar, Lauren A. Juel, Guido F. Verbeck. Metallic nanoparticle production and exposure/deposition system for toxicological research applications using zebrafish. Review of Scientific Instruments 2020, 91 (9) , 094101. https://doi.org/10.1063/5.0013428
  7. Mattea Carmen Castrovilli, Paola Bolognesi, Jacopo Chiarinelli, Lorenzo Avaldi, Antonella Cartoni, Pietro Calandra, Emanuela Tempesta, Maria Teresa Giardi, Amina Antonacci, Fabiana Arduini, Viviana Scognamiglio. Electrospray deposition as a smart technique for laccase immobilisation on carbon black-nanomodified screen-printed electrodes. Biosensors and Bioelectronics 2020, 163 , 112299. https://doi.org/10.1016/j.bios.2020.112299
  8. Alessandra Tata, Chiara Salvitti, Federico Pepi. From vacuum to atmospheric pressure: A review of ambient ion soft landing. International Journal of Mass Spectrometry 2020, 450 , 116309. https://doi.org/10.1016/j.ijms.2020.116309
  9. Rosulek, Darebna, Pompach, Slavata, Novak. Proteases Immobilization for In Situ Time-Limited Proteolysis on MALDI Chips. Catalysts 2019, 9 (10) , 833. https://doi.org/10.3390/catal9100833
  10. Julia Laskin, Grant E. Johnson, Jonas Warneke, Venkateshkumar Prabhakaran. Von isolierten Ionen zu mehrschichtigen funktionellen Materialien durch sanfte Landung von Ionen. Angewandte Chemie 2018, 130 (50) , 16506-16521. https://doi.org/10.1002/ange.201712296
  11. Julia Laskin, Grant E. Johnson, Jonas Warneke, Venkateshkumar Prabhakaran. From Isolated Ions to Multilayer Functional Materials Using Ion Soft Landing. Angewandte Chemie International Edition 2018, 57 (50) , 16270-16284. https://doi.org/10.1002/anie.201712296
  12. Petra Darebna, Jan Spicka, Radek Kucera, Ondrej Topolcan, Eva Navratilova, Viktor Ruzicka, Michael Volny, Petr Novak, Petr Pompach. Detection and Quantification of Carbohydrate-Deficient Transferrin by MALDI-Compatible Protein Chips Prepared by Ambient Ion Soft Landing. Clinical Chemistry 2018, 64 (9) , 1319-1326. https://doi.org/10.1373/clinchem.2017.285452
  13. Helena Kupcova Skalnikova, Jana Cizkova, Jakub Cervenka, Petr Vodicka. Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research. International Journal of Molecular Sciences 2017, 18 (12) , 2697. https://doi.org/10.3390/ijms18122697

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