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Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2018, 90, 11, 6749-6756
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    Specific Detection of Proteins Using Exceptionally Responsive Magnetic Particles
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    • Yi-Ting Chen
      Yi-Ting Chen
      Department of Chemistry, University of Houston, Houston, Texas 77204, United States
      More by Yi-Ting Chen
    • Riddhiman Medhi
      Riddhiman Medhi
      Department of Chemistry, University of Houston, Houston, Texas 77204, United States
      More by Riddhiman Medhi
    • Ivan Nekrashevich
      Ivan Nekrashevich
      Department of Electrical and Computer Engineering, University of Houston, Houston, Texas 77204, United States
      More by Ivan Nekrashevich
    • Dmitri Litvinov
      Dmitri Litvinov
      Department of Chemistry  and  Department of Electrical and Computer Engineering, University of Houston, Houston, Texas 77204, United States
      More by Dmitri Litvinov
      Orcidhttp://orcid.org/0000-0003-2272-562X
    • Shoujun Xu*
      Shoujun Xu
      Department of Chemistry  and  Texas Center for Superconductivity, University of Houston, Houston, Texas 77204, United States
      *E-mail: [email protected]
      More by Shoujun Xu
    • T. Randall Lee*
      T. Randall Lee
      Department of Chemistry  and  Texas Center for Superconductivity, University of Houston, Houston, Texas 77204, United States
      *E-mail: [email protected]
      More by T. Randall Lee
      Orcidhttp://orcid.org/0000-0001-9584-8861
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    Analytical Chemistry

    Cite this: Anal. Chem. 2018, 90, 11, 6749–6756
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    https://doi.org/10.1021/acs.analchem.8b00593
    Published May 7, 2018
    Copyright © 2018 American Chemical Society
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    Abstract

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    Sensitivity and specificity are among the most important parameters for viable sensor technologies based on magnetic nanoparticles. In this work, we describe synthetic routes and analytical approaches to improve both aspects. Magnetic iron oxide particles having diameters of 120, 440, and 700 nm were synthesized, and their surfaces were specifically functionalized. The larger particles showed significantly stronger magnetic signals and responses when compared to commercially available magnetic particles (Dynabeads). A force-based detection method was used to distinguish specifically bound particles (via protein interactions) and nonspecifically bound ones (e.g., via physisorption). In addition, an exchange platform, denoted as exchange-induced remnant magnetization (EXIRM), was developed and utilized to detect label-free proteins specifically. Using EXIRM, the 700 nm magnetic particles showed a 7-fold increase in detection sensitivity when compared to the markedly larger commercially available Dynabeads; furthermore, EXIRM exhibited high specificity, even in a 100-fold increase of nontargeted protein. More generally, particle size effects, reaction times, and dynamic ranges are evaluated and discussed herein.

    Copyright © 2018 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications Web site. Contents include . The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.8b00593.

    • Experimental methods, elemental characterization of the magnetic particles, quantification of magnetization per particle, functionalization route, TEM images after each step of the synthetic procedure, TGA analysis of the functionalized magnetic particles, magnetic properties of the functionalized magnetic particles, and magnetic measurements in protein exchange (PDF)

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    Analytical Chemistry

    Cite this: Anal. Chem. 2018, 90, 11, 6749–6756
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.8b00593
    Published May 7, 2018
    Copyright © 2018 American Chemical Society
    Request reuse permissions

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