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Enhancing Catalytic Activity of Uranyl-Dependent DNAzyme by Flexible Linker Insertion for More Sensitive Detection of Uranyl Ion

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Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2019, 91, 10, 6608-6615
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    Enhancing Catalytic Activity of Uranyl-Dependent DNAzyme by Flexible Linker Insertion for More Sensitive Detection of Uranyl Ion
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    • Mengli Feng
      Mengli Feng
      Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, People’s Republic of China
      More by Mengli Feng
    • Chunmei Gu
      Chunmei Gu
      Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, People’s Republic of China
      More by Chunmei Gu
    • Yanping Sun
      Yanping Sun
      School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, People’s Republic of China
      More by Yanping Sun
    • Shuyuan Zhang
      Shuyuan Zhang
      School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, People’s Republic of China
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    • Aijun Tong
      Aijun Tong
      Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, People’s Republic of China
      More by Aijun Tong
      Orcidhttp://orcid.org/0000-0003-0633-4098
    • Yu Xiang*
      Yu Xiang
      Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, People’s Republic of China
      *E-mail for Y.X.: [email protected]
      More by Yu Xiang
      Orcidhttp://orcid.org/0000-0002-5593-5773
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    Analytical Chemistry

    Cite this: Anal. Chem. 2019, 91, 10, 6608–6615
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    https://doi.org/10.1021/acs.analchem.9b00490
    Published April 24, 2019
    Copyright © 2019 American Chemical Society
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    Abstract

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    The uranyl-dependent DNAzyme 39E cleaves its nucleic acid substrate in the presence of uranyl ion (UO22+). It has been widely utilized in many sensor designs for selective and sensitive detection of UO22+ in the environment and inside live cells. In this work, by inserting a flexible linker (C3 Spacer) into one critical site (A20) of the 39E catalytic core, we successfully enhanced the original catalytic activity of 39E up to 8.1-fold at low UO22+ concentrations. Applying such a modified DNAzyme (39E-A20-C3) in a label-free fluorescent sensor for UO22+ detection achieved more than 1 order of magnitude sensitivity enhancement over using native 39E, with the UO22+ detection limit improved from 2.6 nM (0.63 ppb) to 0.19 nM (0.047 ppb), while the high selectivity to UO22+ over other metal ions was fully preserved. The method was also successfully applied for the detection of UO22+-spiked environmental water samples to demonstrate its practical usefulness.

    Copyright © 2019 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.9b00490.

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    This article is cited by 25 publications.

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    18. Yunlong Bai, Lechang Xu, Huining Chai, Lei Zhou, Guoping Jiang, Guangyao Zhang. Recent Advances on DNAzyme-Based Biosensors for Detection of Uranyl. Frontiers in Chemistry 2022, 10 https://doi.org/10.3389/fchem.2022.882250
    19. Lin Sun, Jinquan Liu, Le Li, Deshuai Zhen, Zhongran Dai, Shuangyang Tang, Bingyu Zhu, Lei Chen, Hong Chen, Mi Gong, Ziwei Tang, Yu Hu. Advances of biosensors for UO22+ detecting based on specific DNAzyme. Inorganic Chemistry Communications 2022, 138 , 109234. https://doi.org/10.1016/j.inoche.2022.109234
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    Analytical Chemistry

    Cite this: Anal. Chem. 2019, 91, 10, 6608–6615
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.9b00490
    Published April 24, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

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