High-Resolution Differential Ion Mobility Separations/Orbitrap Mass Spectrometry without Buffer Gas LimitationsClick to copy article linkArticle link copied!
- Matthew A. BairdMatthew A. BairdDepartment of Chemistry, Wichita State University, 1845 Fairmount, Wichita, Kansas 67260, United StatesMore by Matthew A. Baird
- Pavel V. ShliahaPavel V. ShliahaDepartment of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, DK-5230 Odense M, DenmarkMore by Pavel V. Shliaha
- Gordon A. AndersonGordon A. AndersonGAACE, 101904 Wiser Parkway Suite 105, Kennewick, Washington 99338, United StatesMore by Gordon A. Anderson
- Eugene MoskovetsEugene MoskovetsMassTech Inc., 6992 Columbia Gateway Drive, Columbia, Maryland 21046, United StatesMore by Eugene Moskovets
- Victor LaikoVictor LaikoMassTech Inc., 6992 Columbia Gateway Drive, Columbia, Maryland 21046, United StatesMore by Victor Laiko
- Alexander A. MakarovAlexander A. MakarovThermo Fisher Scientific, Hanna-Kunath Strasse 11, Bremen 28199, GermanyDepartment of Chemistry, University of Utrecht, 3508 TC Utrecht, NetherlandsMore by Alexander A. Makarov
- Ole N. JensenOle N. JensenDepartment of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, DK-5230 Odense M, DenmarkMore by Ole N. Jensen
- Alexandre A. Shvartsburg*Alexandre A. Shvartsburg*E-mail: [email protected]Department of Chemistry, Wichita State University, 1845 Fairmount, Wichita, Kansas 67260, United StatesMore by Alexandre A. Shvartsburg
Abstract
Strong orthogonality between differential ion mobility spectrometry (FAIMS) and mass spectrometry (MS) makes their hybrid a powerful approach to separate isomers and isobars. Harnessing that power depends on high resolution in both dimensions. The ultimate mass resolution and accuracy are delivered by Fourier Transform MS increasingly realized in Orbitrap MS, whereas FAIMS resolution is generally maximized by buffers rich in He or H2 that elevate ion mobility and lead to prominent non-Blanc effects. However, turbomolecular pumps have lower efficiency for light gas molecules and their flow from the FAIMS stage complicates maintaining the ultrahigh vacuum (UHV) needed for Orbitrap operation. Here we address this challenge via two hardware modifications: (i) a differential pumping step between FAIMS and MS stages and (ii) reconfiguration of vacuum lines to isolate pumping of the high vacuum (HV) region. Either greatly ameliorates the pressure increases upon He or H2 aspiration. This development enables free optimization of FAIMS carrier gas without concerns about MS performance, maximizing the utility and flexibility of FAIMS/MS platforms.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 19 publications.
- Alexandre A. Shvartsburg, Pawel Sadowski, Berwyck L. J. Poad, Stephen J. Blanksby. Metal Polycation Adduction to Lipids Enables Superior Ion Mobility Separations with Ultrafast Ozone-Induced Dissociation. Analytical Chemistry 2024, 96
(40)
, 15960-15969. https://doi.org/10.1021/acs.analchem.4c03071
- Junhui Li, Rong Liu, Zhonghan Hu, Shoushuai Fu, Jiancheng Yu, Keqi Tang. Racetrack FAIMS for High-Resolution and High-Sensitivity Characterization of Peptide Conformers. Analytical Chemistry 2024, 96
(34)
, 13980-13986. https://doi.org/10.1021/acs.analchem.4c02750
- Tobias P. Wörner, Hayden A. Thurman, Alexander A. Makarov, Alexandre A. Shvartsburg. Expanding Differential Ion Mobility Separations into the MegaDalton Range. Analytical Chemistry 2024, 96
(14)
, 5392-5398. https://doi.org/10.1021/acs.analchem.3c05012
- Pratima Pathak, Alexandre A. Shvartsburg. High-Definition Ion Mobility/Mass Spectrometry with Structural Isotopic Shifts for Nominally Isobaric Isotopologues. The Journal of Physical Chemistry A 2023, 127
(17)
, 3914-3923. https://doi.org/10.1021/acs.jpca.3c01792
- Junhui Li, Lei Li, Wenqing Gao, Shoudong Shi, Jiancheng Yu, Keqi Tang. Two-Dimensional FAIMS-IMS Characterization of Peptide Conformers with Resolution Exceeding 1000. Analytical Chemistry 2022, 94
(16)
, 6363-6370. https://doi.org/10.1021/acs.analchem.2c00805
- Kevin Jeanne Dit Fouque, Desmond Kaplan, Valery G. Voinov, Frederik H. V. Holck, Ole N. Jensen, Francisco Fernandez-Lima. Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Analytical Chemistry 2021, 93
(27)
, 9575-9582. https://doi.org/10.1021/acs.analchem.1c01735
- Francis Berthias, Matthew A. Baird, Alexandre A. Shvartsburg. Differential Ion Mobility Separations of d/l Peptide Epimers. Analytical Chemistry 2021, 93
(8)
, 4015-4022. https://doi.org/10.1021/acs.analchem.0c05023
- Pavel V. Shliaha, Vladimir Gorshkov, Sergey I. Kovalchuk, Veit Schwämmle, Matthew A. Baird, Alexandre A. Shvartsburg, Ole N. Jensen. Middle-Down Proteomic Analyses with Ion Mobility Separations of Endogenous Isomeric Proteoforms. Analytical Chemistry 2020, 92
(3)
, 2364-2368. https://doi.org/10.1021/acs.analchem.9b05011
- Daniel
L. Winter, Jordan Mastellone, K. M. Mohibul Kabir, Marc R. Wilkins, William A. Donald. Separation of Isobaric Mono- and Dimethylated RGG-Repeat Peptides by Differential Ion Mobility-Mass Spectrometry. Analytical Chemistry 2019, 91
(18)
, 11827-11833. https://doi.org/10.1021/acs.analchem.9b02504
- Shoushuai Fu, Chenlu Wang, Junhui Li, Jiancheng Yu, Keqi Tang. Simulation study of a new racetrack FAIMS analyzer to achieve both high-resolution and high-sensitivity. Talanta 2024, 276 , 126305. https://doi.org/10.1016/j.talanta.2024.126305
- Jie Hao, Rong Feng, Junhui Li, Wenqing Gao, Jiancheng Yu, Keqi Tang. A high-performance standalone planar FAIMS system for effective detection of chemical warfare agents via TSPSO algorithm. Talanta 2024, 269 , 125516. https://doi.org/10.1016/j.talanta.2023.125516
- Xiaoli Ji, Rong Liu, Jie Hao, Chenlu Wang, Junhui Li, Wenqing Gao, Jiancheng Yu, Keqi Tang. Two‐step particle swarm optimization algorithm for effective deconvolution and resolution enhancement of various overlapping peaks. Rapid Communications in Mass Spectrometry 2023, 37
(3)
https://doi.org/10.1002/rcm.9429
- Ayoub Boulghobra, Myriam Bonose, Eskandar Alhajji, Antoine Pallandre, Emmanuel Flamand-Roze, Bruno Baudin, Marie-Claude Menet, Fathi Moussa. Autoxidation Kinetics of Tetrahydrobiopterin—Giving Quinonoid Dihydrobiopterin the Consideration It Deserves. Molecules 2023, 28
(3)
, 1267. https://doi.org/10.3390/molecules28031267
- Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li. High-end ion mobility mass spectrometry: A current review of analytical capacity in omics applications and structural investigations. TrAC Trends in Analytical Chemistry 2022, 157 , 116761. https://doi.org/10.1016/j.trac.2022.116761
- Junhui Li, Wenqing Gao, Huanming Wu, Shoudong Shi, Jiancheng Yu, Keqi Tang. Application of zero‐phase digital filtering for effective denoising of field asymmetric waveform ion mobility spectrometry signal. Rapid Communications in Mass Spectrometry 2022, 36
(1)
https://doi.org/10.1002/rcm.9211
- Junhui Li, Wenqing Gao, Huanming Wu, Shoudong Shi, Jiancheng Yu, Keqi Tang. On the resolution, sensitivity and ion transmission efficiency of a planar FAIMS. International Journal of Mass Spectrometry 2022, 471 , 116727. https://doi.org/10.1016/j.ijms.2021.116727
- James D. Sanders, Jamie P. Butalewicz, Brian H. Clowers, Jennifer S. Brodbelt. Absorption Mode Fourier Transform Ion Mobility Mass Spectrometry Multiplexing Combined with Half-Window Apodization Windows Improves Resolution and Shortens Acquisition Times. Analytical Chemistry 2021, 93
(27)
, 9513-9520. https://doi.org/10.1021/acs.analchem.1c01427
- Francis Berthias, Yali Wang, Eskander Alhajji, Bernard Rieul, Fathi Moussa, Jean-François Benoist, Philippe Maître. Identification and quantification of amino acids and related compounds based on Differential Mobility Spectrometry. The Analyst 2020, 145
(14)
, 4889-4900. https://doi.org/10.1039/D0AN00377H
- Pratima Pathak, Matthew A. Baird, Alexandre A. Shvartsburg. High-Resolution Ion Mobility Separations of Isomeric Glycoforms with Variations on the Peptide and Glycan Levels. Journal of the American Society for Mass Spectrometry 2020, 31
(7)
, 1603-1609. https://doi.org/10.1021/jasms.0c00183
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.