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Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures

  • Roberto Fernández
    Roberto Fernández
    Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
    Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
  • Jone Garate
    Jone Garate
    Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
    More by Jone Garate
  • Tarson Tolentino-Cortez
    Tarson Tolentino-Cortez
    Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
  • Ainara Herraiz
    Ainara Herraiz
    Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
  • Laura Lombardero
    Laura Lombardero
    Department of Pharmacology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
  • Fabien Ducrocq
    Fabien Ducrocq
    University of Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France
  • Rafael Rodríguez-Puertas
    Rafael Rodríguez-Puertas
    Department of Pharmacology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
    Neurodegenerative Diseases, Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain
  • Pierre Trifilieff
    Pierre Trifilieff
    University of Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France
  • Egoitz Astigarraga
    Egoitz Astigarraga
    Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
  • Gabriel Barreda-Gómez*
    Gabriel Barreda-Gómez
    Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
    *Phone: +34944316577. E-mail: [email protected]
  • , and 
  • José A. Fernández*
    José A. Fernández
    Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
    *Phone: +34946015387. Fax: +34946013500. E-mail: [email protected]
Cite this: Anal. Chem. 2019, 91, 24, 15967–15973
Publication Date (Web):November 21, 2019
https://doi.org/10.1021/acs.analchem.9b04529
Copyright © 2019 American Chemical Society

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    Abstract

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    The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell’s homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets.

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    • Additional figures of tissue classification and correlation between lipid species; tables with tissue classification by comparison with the lipid fingerprint of the tissues in the rat array; table with the species that experience the largest changes between both mice models (PDF)

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