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Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2019, 91, 24, 15967-15973
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    Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures
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    • Roberto Fernández
      Roberto Fernández
      Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
      Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
      More by Roberto Fernández
    • Jone Garate
      Jone Garate
      Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
      More by Jone Garate
    • Tarson Tolentino-Cortez
      Tarson Tolentino-Cortez
      Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
      More by Tarson Tolentino-Cortez
    • Ainara Herraiz
      Ainara Herraiz
      Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
      More by Ainara Herraiz
    • Laura Lombardero
      Laura Lombardero
      Department of Pharmacology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
      More by Laura Lombardero
    • Fabien Ducrocq
      Fabien Ducrocq
      University of Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France
      More by Fabien Ducrocq
    • Rafael Rodríguez-Puertas
      Rafael Rodríguez-Puertas
      Department of Pharmacology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
      Neurodegenerative Diseases, Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain
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    • Pierre Trifilieff
      Pierre Trifilieff
      University of Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France
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    • Egoitz Astigarraga
      Egoitz Astigarraga
      Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
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    • Gabriel Barreda-Gómez*
      Gabriel Barreda-Gómez
      Research Department, IMG Pharma Biotech S.L., BIC Bizkaia (612), 48160-Derio, Spain
      *Phone: +34944316577. E-mail: [email protected]
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    • José A. Fernández*
      José A. Fernández
      Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, Leioa 48940, Spain
      *Phone: +34946015387. Fax: +34946013500. E-mail: [email protected]
      More by José A. Fernández
      Orcidhttp://orcid.org/0000-0002-7315-2326
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    Analytical Chemistry

    Cite this: Anal. Chem. 2019, 91, 24, 15967–15973
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.9b04529
    Published November 21, 2019
    Copyright © 2019 American Chemical Society
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    Abstract

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    The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell’s homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets.

    Copyright © 2019 American Chemical Society

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.9b04529.

    • Additional figures of tissue classification and correlation between lipid species; tables with tissue classification by comparison with the lipid fingerprint of the tissues in the rat array; table with the species that experience the largest changes between both mice models (PDF)

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    This article is cited by 21 publications.

    1. Laura Sánchez-Sánchez, Roberto Fernández, Egoitz Astigarraga, Gabriel Barreda-Gómez, María Dolores Ganfornina. Microarray-Based Methodology for Lipid Profiling, Enzymatic Activity, And Binding Assays in Printed Lipid Raft Membranes from Astrocytes and Neurons. Analytical Chemistry 2025, 97 (1) , 86-95. https://doi.org/10.1021/acs.analchem.4c02421
    2. Lucía Martín-Saiz, Beatriz Abad-García, Jon D. Solano-Iturri, Lorena Mosteiro, Javier Martín-Allende, Yuri Rueda, Amparo Pérez-Fernández, Miguel Unda, Pedro Coterón-Ochoa, Aintzane Goya, Alberto Saiz, Jennifer Martínez, Begoña Ochoa, Olatz Fresnedo, Gorka Larrinaga, José A. Fernández. Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma. Analytical Chemistry 2023, 95 (4) , 2285-2293. https://doi.org/10.1021/acs.analchem.2c03953
    3. Lucía Martín-Saiz, Lorena Mosteiro, Jon D. Solano-Iturri, Yuri Rueda, Javier Martín-Allende, Igone Imaz, Iván Olano, Begoña Ochoa, Olatz Fresnedo, José A. Fernández, Gorka Larrinaga. High-Resolution Human Kidney Molecular Histology by Imaging Mass Spectrometry of Lipids. Analytical Chemistry 2021, 93 (27) , 9364-9372. https://doi.org/10.1021/acs.analchem.1c00649
    4. Simon F. Berlanda, Maximilian Breitfeld, Claudius L. Dietsche, Petra S. Dittrich. Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics. Analytical Chemistry 2021, 93 (1) , 311-331. https://doi.org/10.1021/acs.analchem.0c04366
    5. Albert Maimó-Barceló, Lucía Martín-Saiz, Maria Barceló-Nicolau, Simona Salivo, Karim Pérez-Romero, Ramon M. Rodriguez, Javier Martín, Marco A. Martínez, Marcelo García, Isabel Amengual, Daniel Ginard, José A. Fernández, Gwendolyn Barceló-Coblijn. Lipid signature associated with chronic colon inflammation reveals a dysregulation in colonocyte differentiation process. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2024, 1869 (7) , 159528. https://doi.org/10.1016/j.bbalip.2024.159528
    6. Ibai Calvo, Alejandro Montilla, Cristina Huergo, Lucía Martín-Saiz, Javier Martín-Allende, Vanja Tepavcevic, María Domercq, José A. Fernández. Combining imaging mass spectrometry and immunohistochemistry to analyse the lipidome of spinal cord inflammation. Analytical and Bioanalytical Chemistry 2024, 416 (8) , 1923-1933. https://doi.org/10.1007/s00216-024-05190-3
    7. Cristina Huergo‐Baños, Verónica Velasco, Jone Garate, Roberto Fernández, Javier Martín‐Allende, Ignacio Zabalza, Juan L. Artola, Rosa M. Martí, Aintzane Asumendi, Egoitz Astigarraga, Gabriel Barreda‐Gómez, Olatz Fresnedo, Begoña Ochoa, Maria D. Boyano, José A. Fernández. Lipid fingerprint‐based histology accurately classifies nevus, primary melanoma, and metastatic melanoma samples. International Journal of Cancer 2024, 154 (4) , 712-722. https://doi.org/10.1002/ijc.34800
    8. Leidi Hernandez-Suarez, Eguzkiñe Diez-Martin, June Egiguren-Ortiz, Roberto Fernandez, Aitor Etxebarria, Egoitz Astigarraga, Cristina Miguelez, Andoni Ramirez-Garcia, Gabriel Barreda-Gómez. Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders. International Journal of Molecular Sciences 2024, 25 (4) , 2025. https://doi.org/10.3390/ijms25042025
    9. Marie-Lise Jobin, Véronique De Smedt-Peyrusse, Fabien Ducrocq, Rim Baccouch, Asma Oummadi, Maria Hauge Pedersen, Brian Medel-Lacruz, Maria-Florencia Angelo, Sandrine Villette, Pierre Van Delft, Laetitia Fouillen, Sébastien Mongrand, Jana Selent, Tarson Tolentino-Cortez, Gabriel Barreda-Gómez, Stéphane Grégoire, Elodie Masson, Thierry Durroux, Jonathan A. Javitch, Ramon Guixà-González, Isabel D. Alves, Pierre Trifilieff. Impact of membrane lipid polyunsaturation on dopamine D2 receptor ligand binding and signaling. Molecular Psychiatry 2023, 28 (5) , 1960-1969. https://doi.org/10.1038/s41380-022-01928-6
    10. María Dolores García-Fernández, Ane Larrea, Roberto Fernández, Rafael Rodríguez-Puertas, Egoitz Astigarraga, Iván Manuel, Gabriel Barreda-Gómez. Microarrays, Enzymatic Assays, and MALDI-MS for Determining Specific Alterations to Mitochondrial Electron Transport Chain Activity, ROS Formation, and Lipid Composition in a Monkey Model of Parkinson’s Disease. International Journal of Molecular Sciences 2023, 24 (6) , 5470. https://doi.org/10.3390/ijms24065470
    11. Yaiza Garro-Aguilar, Roberto Fernández, Silvia Calero, Ekaterina Noskova, Marina Gulak, Miguel de la Fuente, Albert Adell, Edurne Simón, Urko Muzquiz, Diego Rodríguez-Piñón, Egoitz Astigarraga, Gabriel Barreda-Gómez. Acute Stress-Induced Changes in the Lipid Composition of Cow’s Milk in Healthy and Pathological Animals. Molecules 2023, 28 (3) , 980. https://doi.org/10.3390/molecules28030980
    12. Laura Sánchez-Sánchez, Roberto Fernández, Maria Dolores Ganfornina, Egoitz Astigarraga, Gabriel Barreda-Gómez. Protective Actions of α-Tocopherol on Cell Membrane Lipids of Paraquat-Stressed Human Astrocytes Using Microarray Technology, MALDI-MS and Lipidomic Analysis. Antioxidants 2022, 11 (12) , 2440. https://doi.org/10.3390/antiox11122440
    13. Aitor Etxebarria, Eguzkiñe Díez-Martín, Egoitz Astigarraga, Gabriel Barreda-Gómez. Role of the Immune System in Renal Transplantation, Types of Response, Technical Approaches and Current Challenges. Immuno 2022, 2 (4) , 548-570. https://doi.org/10.3390/immuno2040035
    14. Lucía Martín‐Saiz, Juan Guerrero‐Mauvecin, Diego Martín‐Sanchez, Olatz Fresnedo, Manuel J Gómez, Susana Carrasco, Pablo Cannata‐Ortiz, Alberto Ortiz, José A Fernandez, Ana B Sanz. Ferrostatin‐1 modulates dysregulated kidney lipids in acute kidney injury. The Journal of Pathology 2022, 257 (3) , 285-299. https://doi.org/10.1002/path.5882
    15. Ane Elexpe, Laura Sánchez-Sánchez, Tarson Tolentino-Cortez, Egoitz Astigarraga, María Torrecilla, Gabriel Barreda-Gómez. Analysis of Mitochondrial Function in Cell Membranes as Indicator of Tissue Vulnerability to Drugs in Humans. Biomedicines 2022, 10 (5) , 980. https://doi.org/10.3390/biomedicines10050980
    16. Miguel de la Fuente, Iñaki Rodríguez-Agirretxe, Elena Vecino, Egoitz Astigarraga, Arantxa Acera, Gabriel Barreda-Gómez. Elevation of Tear MMP-9 Concentration as a Biomarker of Inflammation in Ocular Pathology by Antibody Microarray Immunodetection Assays. International Journal of Molecular Sciences 2022, 23 (10) , 5639. https://doi.org/10.3390/ijms23105639
    17. Lissete Sánchez-Magraner, Miguel de la Fuente, Charles Evans, James Miles, Ane Elexpe, Maddalen Rodriguez-Astigarraga, Egoitz Astigarraga, Gabriel Barreda-Gómez. Quantification of PD-1/PD-L1 Interaction between Membranes from PBMCs and Melanoma Samples Using Cell Membrane Microarray and Time-Resolved Förster Resonance Energy Transfer. Analytica 2021, 2 (4) , 156-170. https://doi.org/10.3390/analytica2040015
    18. Ane Elexpe, Nerea Nieto, Claudia Fernández-Cuétara, Celtia Domínguez-Fernández, Teresa Morera-Herreras, María Torrecilla, Cristina Miguélez, Antonio Laso, Eneko Ochoa, María Bailen, Azucena González-Coloma, Iñigo Angulo-Barturen, Egoitz Astigarraga, Gabriel Barreda-Gómez. Study of Tissue-Specific Reactive Oxygen Species Formation by Cell Membrane Microarrays for the Characterization of Bioactive Compounds. Membranes 2021, 11 (12) , 943. https://doi.org/10.3390/membranes11120943
    19. Miguel de la Fuente, Laura Lombardero, Alfonso Gómez-González, Cristina Solari, Iñigo Angulo-Barturen, Arantxa Acera, Elena Vecino, Egoitz Astigarraga, Gabriel Barreda-Gómez. Enzyme Therapy: Current Challenges and Future Perspectives. International Journal of Molecular Sciences 2021, 22 (17) , 9181. https://doi.org/10.3390/ijms22179181
    20. Bárbara Rienda, Ane Elexpe, Tarson Tolentino-Cortez, Marina Gulak, Cristina Bruzos-Cidón, María Torrecilla, Egoitz Astigarraga, Gabriel Barreda-Gómez. Analysis of Acetylcholinesterase Activity in Cell Membrane Microarrays of Brain Areas as a Screening Tool to Identify Tissue Specific Inhibitors. Analytica 2021, 2 (1) , 25-36. https://doi.org/10.3390/analytica2010003
    21. Xandra Pereiro, Roberto Fernández, Gabriel Barreda-Gómez, Noelia Ruzafa, Arantxa Acera, Javier Araiz, Egoitz Astigarraga, Elena Vecino. Comparative lipidomic analysis of mammalian retinal ganglion cells and Müller glia in situ and in vitro using High-Resolution Imaging Mass Spectrometry. Scientific Reports 2020, 10 (1) https://doi.org/10.1038/s41598-020-77087-x
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    Analytical Chemistry

    Cite this: Anal. Chem. 2019, 91, 24, 15967–15973
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.9b04529
    Published November 21, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

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