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Nuclear Pore Membrane Proteins Self-Assemble into Nanopores

  • Radhakrishnan Panatala
    Radhakrishnan Panatala
    Biozentrum and Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland
  • Suncica Barbato
    Suncica Barbato
    Biozentrum and Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland
  • Toshiya Kozai
    Toshiya Kozai
    Biozentrum and Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland
  • Jinghui Luo
    Jinghui Luo
    Paul Scherrer Institute, 5232 Villigen, Switzerland
    More by Jinghui Luo
  • Larisa E. Kapinos
    Larisa E. Kapinos
    Biozentrum and Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland
  • , and 
  • Roderick Y. H. Lim*
    Roderick Y. H. Lim
    Biozentrum and Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland
    *E-mail: [email protected]. Phone: +41 61 207 2083. Fax: +41 61 207 2109.
Cite this: Biochemistry 2019, 58, 6, 484–488
Publication Date (Web):January 3, 2019
https://doi.org/10.1021/acs.biochem.8b01179
Copyright © 2019 American Chemical Society

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    Abstract

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    Large multiprotein nanopores remain difficult to reconstitute in vitro, such as, for instance, the nuclear pore complex (NPC) that regulates macromolecular transport between the nucleus and cytoplasm in cells. Here, we report that two NPC pore membrane proteins self-assemble into ∼20 nm diameter nanopores following in vitro reconstitution into lipid bilayers. Pore formation follows from the assembly of Pom121 and Ndc1 oligomers, which arrange into ringlike membrane structures that encircle aqueous, electrically conductive pores. This represents a key step toward reconstituting membrane-embedded NPC mimics for biological studies and biotechnological applications.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.biochem.8b01179.

    • Supplementary figures for Figures 1, 3, and 4, Figures S5 and S6 with their respective figure legends, and Materials and Methods (PDF)

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    Cited By

    This article is cited by 3 publications.

    1. Qian Shen, Junchen Li, Chuanlong Zhang, Xue Pan, Yi Li, Xiyuan Zhang, Ge’er En, Bo Pang. Pan-cancer analysis and experimental validation identify ndc1 as a potential immunological, prognostic and therapeutic biomarker in pancreatic cancer. Aging 2023, 15 (18) , 9779-9796. https://doi.org/10.18632/aging.205048
    2. Alexandr A. Makarov, Norma E. Padilla-Mejia, Mark C. Field. Evolution and diversification of the nuclear pore complex. Biochemical Society Transactions 2021, 49 (4) , 1601-1619. https://doi.org/10.1042/BST20200570
    3. Bart W. Hoogenboom, Loren E. Hough, Edward A. Lemke, Roderick Y.H. Lim, Patrick R. Onck, Anton Zilman. Physics of the nuclear pore complex: Theory, modeling and experiment. Physics Reports 2021, 921 , 1-53. https://doi.org/10.1016/j.physrep.2021.03.003

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