Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C BiosynthesisClick to copy article linkArticle link copied!
- Hai P. NguyenHai P. NguyenDepartment of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, United StatesMore by Hai P. Nguyen
- Kenichi Yokoyama*Kenichi Yokoyama*E-mail: [email protected]Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, United StatesDepartment of Chemistry, Duke University, Durham, North Carolina 27708-0354, United StatesMore by Kenichi Yokoyama
Abstract

Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis remained uncharacterized. Here we report evidence that AHBA is first loaded onto an MmcB acyl carrier protein (ACP) by a MitE acyl ACP synthetase, followed by a transfer of GlcNAc from UDP-GlcNAc by MitB. The results suggest that the early steps of mitomycin biosynthesis proceed via intermediates linked to MmcB.
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