ACS Publications. Most Trusted. Most Cited. Most Read
Label-Free Detection and Discrimination of Bacterial Pathogens Based on Hemin Recognition

OR SEARCH CITATIONS

My Activity
Publications

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:Bioconjugate Chem. 2016, 27, 7, 1713-1722
    Article

    Label-Free Detection and Discrimination of Bacterial Pathogens Based on Hemin Recognition
    Click to copy article linkArticle link copied!

    View Author Information
    † †Department of Chemistry and ‡Department of Comparative Pathology and Biochemistry, Purdue University, West Lafayette, Indiana 47907, United States
    *E-mail: [email protected]
    Other Access Options

    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2016, 27, 7, 1713–1722
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.bioconjchem.6b00236
    Published June 23, 2016
    Copyright © 2016 American Chemical Society
    Request reuse permissions

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    Hemin linked to hexa(ethylene glycol)bishydrazide was patterned by inkjet printing into periodic microarrays, and evaluated for their ability to capture bacterial pathogens expressing various hemin receptors. Bacterial adhesion was imaged under darkfield conditions with Fourier analysis, supporting a label-free method of pathogen detection. Hemin microarrays were screened against a panel of 16 bacteria and found capable of capturing multiple species, some with limits of detection as low as 103 cfu/mL. Several Gram-positive strains including Staphylococcus aureus and Bacillus anthracis also exhibited rapid adhesion, enabling pattern recognition within minutes of exposure. This can be attributed to differences in hemin acquisition systems: aggressively adherent bacteria express cell-surface hemin receptors (CSHRs) that enable direct hemin binding and uptake, whereas other types of bacteria including most Gram-negative strains rely on the secretion and recapture of soluble proteins (hemophores) for hemin acquisition, with consequently longer times for ligand binding and detection.

    Copyright © 2016 American Chemical Society

    Subjects

    what are subjects

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai
    powered by  Scite

    This article is cited by 6 publications.

    1. Neetu Tripathi, Manoj Kumar Goshisht. Recent Advances and Mechanistic Insights into Antibacterial Activity, Antibiofilm Activity, and Cytotoxicity of Silver Nanoparticles. ACS Applied Bio Materials 2022, 5 (4) , 1391-1463. https://doi.org/10.1021/acsabm.2c00014
    2. Ana V. Morales-de-Echegaray, Thora R. Maltais, Lu Lin, Waleed Younis, Naveen R. Kadasala, Mohamed N. Seleem, Alexander Wei. Rapid Uptake and Photodynamic Inactivation of Staphylococci by Ga(III)-Protoporphyrin IX. ACS Infectious Diseases 2018, 4 (11) , 1564-1573. https://doi.org/10.1021/acsinfecdis.8b00125
    3. Muhammed Awad, Timothy J. Barnes, Nicky Thomas, Paul Joyce, Clive A. Prestidge. Gallium Protoporphyrin Liquid Crystalline Lipid Nanoparticles: A Third-Generation Photosensitizer against Pseudomonas aeruginosa Biofilms. Pharmaceutics 2022, 14 (10) , 2124. https://doi.org/10.3390/pharmaceutics14102124
    4. Muhammed Awad, Timothy J. Barnes, Paul Joyce, Nicky Thomas, Clive A. Prestidge. Liquid crystalline lipid nanoparticle promotes the photodynamic activity of gallium protoporphyrin against S. aureus biofilms. Journal of Photochemistry and Photobiology B: Biology 2022, 232 , 112474. https://doi.org/10.1016/j.jphotobiol.2022.112474
    5. Ana V. Morales-de-Echegaray, Lu Lin, Badhu Sivasubramaniam, Aiganym Yermembetova, Qi Wang, Nader S. Abutaleb, Mohamed N. Seleem, Alexander Wei. Antimicrobial photodynamic activity of gallium-substituted haemoglobin on silver nanoparticles. Nanoscale 2020, 12 (42) , 21734-21742. https://doi.org/10.1039/C9NR09064A
    6. Mengyao Wang, Yue Wu, Yong He, Xiaoxiao Su, Hui Ouyang, Zhifeng Fu. Antibiotic-affinity strategy for bioluminescent detection of viable Gram-positive bacteria using daptomycin as recognition agent. Analytica Chimica Acta 2017, 987 , 91-97. https://doi.org/10.1016/j.aca.2017.08.030
    Download PDF
    Go to Bioconjugate Chemistry
    Get e-Alerts
    Get e-Alerts

    Bioconjugate Chemistry

    Cite this: Bioconjugate Chem. 2016, 27, 7, 1713–1722
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.bioconjchem.6b00236
    Published June 23, 2016
    Copyright © 2016 American Chemical Society
    Request reuse permissions

    Article Views

    684

    Altmetric

    -

    Citations

    6
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.