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Anionically Functionalized Glycogen Encapsulates Melittin by Multivalent Interaction

  • Hanna Zhukouskaya
    Hanna Zhukouskaya
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Pablo M. Blanco
    Pablo M. Blanco
    Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, Prague 2 128 40, Czech Republic
  • Zulfiya Černochová
    Zulfiya Černochová
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Lucie Čtveráčková
    Lucie Čtveráčková
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Roman Staňo
    Roman Staňo
    Faculty of Physics, University of Vienna, Kolingasse 14-16, Vienna 1090, Austria
    More by Roman Staňo
  • Ewa Pavlova
    Ewa Pavlova
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
    More by Ewa Pavlova
  • Miroslav Vetrík
    Miroslav Vetrík
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Peter Černoch
    Peter Černoch
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Marcela Filipová
    Marcela Filipová
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Miroslav Šlouf
    Miroslav Šlouf
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Miroslav Štěpánek
    Miroslav Štěpánek
    Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, Prague 2 128 40, Czech Republic
  • Martin Hrubý
    Martin Hrubý
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
  • Peter Košovan*
    Peter Košovan
    Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, Prague 2 128 40, Czech Republic
    *Email: [email protected]
  • , and 
  • Jiří Pánek*
    Jiří Pánek
    Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 06, Czech Republic
    *Email: [email protected]
Cite this: Biomacromolecules 2022, 23, 8, 3371–3382
Publication Date (Web):June 29, 2022
https://doi.org/10.1021/acs.biomac.2c00400
Copyright © 2022 American Chemical Society

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    Abstract

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    We developed acid-functionalized glycogen conjugates as supramolecular carriers for efficient encapsulation and inhibition of a model cationic peptide melittin─the main component of honeybee venom. For this purpose, we synthesized and characterized a set of glycogens, functionalized to various degrees by several different acid groups. These conjugates encapsulate melittin up to a certain threshold amount, beyond which they precipitate. Computer simulations showed that sufficiently functionalized conjugates electrostatically attract melittin, resulting in its efficient encapsulation in a broad pH range around the physiological pH. Hemolytic assays confirmed in vitro that the effective inhibition of melittin’s hemolytic activity occurs for highly functionalized samples, whereas no inhibition is observed when using low-functionalized conjugates. It can be concluded that functional glycogens are promising carriers for cationic molecular cargos or antidotes against animal venoms under conditions, in which suitable properties such as biodegradability and biocompatibility are crucial.

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.biomac.2c00400.

    • Details on the computational model; additional details on characterization of the prepared glycogen conjugates by ATR-FTIR, 1H NMR, DLS, and ζ potential measurements (PDF)

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    Cited By

    This article is cited by 1 publications.

    1. Zulfiya Černochová, Volodymyr Lobaz, Lucie Čtveráčková, Peter Černoch, Miroslav Šlouf, Marcela Filipová, Martin Hrubý, Jiří Pánek. Encapsulating melittin from animal venom by finely tuned charge compensation with polymer carriers. European Polymer Journal 2023, 190 , 111996. https://doi.org/10.1016/j.eurpolymj.2023.111996

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