Recombinant Spider Silk Bioinks for Continuous Protein Release by Encapsulated Producer CellsClick to copy article linkArticle link copied!
- Vanessa T. TrossmannVanessa T. TrossmannLehrstuhl Biomaterialien, Fakultät für Ingenieurswissenschaften, Universität Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, Bayreuth 95447, GermanyMore by Vanessa T. Trossmann
- Stefanie Heltmann-MeyerStefanie Heltmann-MeyerDepartment of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Krankenhaus-Str. 12, Erlangen 91054, GermanyMore by Stefanie Heltmann-Meyer
- Hanna AmoueiHanna AmoueiDivision of Molecular Internal Medicine, Department of Internal Medicine II, University Hospital of Würzburg, Grombühl-Str. 12, Würzburg 97080, GermanyMore by Hanna Amouei
- Harald WajantHarald WajantDivision of Molecular Internal Medicine, Department of Internal Medicine II, University Hospital of Würzburg, Grombühl-Str. 12, Würzburg 97080, GermanyMore by Harald Wajant
- Raymund E. HorchRaymund E. HorchDepartment of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Krankenhaus-Str. 12, Erlangen 91054, GermanyMore by Raymund E. Horch
- Dominik Steiner*Dominik Steiner*Email: [email protected]. Phone: +49 (0) 9131 85 33 296.Department of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Krankenhaus-Str. 12, Erlangen 91054, GermanyMore by Dominik Steiner
- Thomas Scheibel*Thomas Scheibel*Email: [email protected]. Phone: +49 (0) 921 55 6700Lehrstuhl Biomaterialien, Fakultät für Ingenieurswissenschaften, Universität Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, Bayreuth 95447, GermanyBayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayerisches Polymerinstitut (BPI), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Universität Bayreuth, Universitäts-Str. 30, Bayreuth 95447, GermanyMore by Thomas Scheibel
Abstract
Targeted therapies using biopharmaceuticals are of growing clinical importance in disease treatment. Currently, there are several limitations of protein-based therapeutics (biologicals), including suboptimal biodistribution, lack of stability, and systemic side effects. A promising approach to overcoming these limitations could be a therapeutic cell-loaded 3D construct consisting of a suitable matrix component that harbors producer cells continuously secreting the biological of interest. Here, the recombinant spider silk proteins eADF4(C16), eADF4(C16)-RGD, and eADF4(C16)-RGE have been processed together with HEK293 producer cells stably secreting the highly traceable reporter biological TNFR2-Fc-GpL, a fusion protein consisting of the extracellular domain of TNFR2, the Fc domain of human IgG1, and the luciferase of Gaussia princeps as a reporter domain. eADF4(C16) and eADF4(C16)-RGD hydrogels provide structural and mechanical support, promote HEK293 cell growth, and allow fusion protein production by the latter. Bioink-captured HEK293 producer cells continuously release functional TNFR2-Fc-GpL over 14 days. Thus, the combination of biocompatible, printable spider silk bioinks with drug-producing cells is promising for generating implantable 3D constructs for continuous targeted therapy.
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This article is cited by 10 publications.
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- Vanessa T. Trossmann, Thomas Scheibel. Spider silk and blend biomaterials: recent advances and future opportunities. 2024, 133-190. https://doi.org/10.1016/B978-0-323-96017-5.00005-4
- Eduarda P. Oliveira, F. Raquel Maia, Rui L. Reis, Joaquim M. Oliveira. Engineering enzymatic- and photo-crosslinked silk-based hydrogels for regenerative medicine. 2024, 221-239. https://doi.org/10.1016/B978-0-323-96017-5.00009-1
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