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Size-Limited Penetration of Nanoparticles into Porcine Respiratory Mucus after Aerosol Deposition

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    Size-Limited Penetration of Nanoparticles into Porcine Respiratory Mucus after Aerosol Deposition
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    ‡ ∥ ‡Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), ∥Biopharmaceutics and Pharmaceutical Technology, Department of Pharmacy, and ⊥Experimental Physics, Saarland University, 66123 Saarbruecken, Germany
    § Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany
    *E-mail: [email protected]. Tel.: +49 681 98806-1000.
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    Biomacromolecules

    Cite this: Biomacromolecules 2016, 17, 4, 1536–1542
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    https://doi.org/10.1021/acs.biomac.6b00164
    Published March 9, 2016
    Copyright © 2016 American Chemical Society
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    Abstract

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    We investigated the rheological properties and the penetration of differently sized carboxylated nanoparticles in pig pulmonary mucus, on different distance and time scales. Nanoparticles were either mechanically mixed into the mucus samples or deposited as an aerosol, the latter resembling a more physiologically relevant delivery scenario. After mechanical dispersion, 500 nm particles were locally trapped; a fraction of carboxylated tracer particles of 100 or 200 nm in diameter could however freely diffuse in these networks over distances of approximately 20 μm. In contrast, after aerosol deposition on top of the mucus layer only particles with a size of 100 nm were able to penetrate into mucus, suggesting the presence of smaller pores at the air-mucus interface compared to within mucus. These findings are relevant to an understanding of the fate of potentially harmful aerosol particles, such as pathogens, pollutants, and other nanomaterials after incidental inhalation, as well as for the design of pulmonary drug delivery systems.

    Copyright © 2016 American Chemical Society

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.biomac.6b00164.

    • Calibration curves showing a strong linear correlation between the concentration and fluorescence intensity of the fluorescently labeled particles; Comparison of the MSD plots of 100, 200, and 500 nm carboxylated nanoparticles in fresh pulmonary pig mucus, versus pig mucus stored at −20 °C and thawed gradually (PDF).

    • Video-recording of 100 nm COOH-modified particles in native porcine pulmonary mucus (MPG).

    • Video-recording of 200 nm COOH-modified particles in native porcine pulmonary mucus (MPG).

    • Video-recording of 500 nm COOH-modified particles in native porcine pulmonary mucus (MPG).

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    Cite this: Biomacromolecules 2016, 17, 4, 1536–1542
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    https://doi.org/10.1021/acs.biomac.6b00164
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