Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules
- Celia C. Homyak
- ,
- Ann Fernandez
- ,
- Mollie A. Touve
- ,
- Bo Zhao
- ,
- Francesca Anson
- ,
- Jeanne A. Hardy
- ,
- Richard W. Vachet
- ,
- Nathan C. Gianneschi
- ,
- Jennifer L. Ross
- , and
- S. Thayumanavan
Abstract
Lipid–polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV–vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.
Cited By
This article is cited by 8 publications.
- Xunan Wei, Yujie Li, Xingdi Cheng, Yixing Wen, Wei Yuan, Ruofan Chen, Siwen Meng, Xueguang Lu, Zhiyong Yu, Lijin Xu, Dongsheng Liu, Yuanchen Dong. Increase Nebulization Stability of Lipid Nanoparticles by Integrating a DNA Supramolecular Hydrogel. ACS Macro Letters 2023, 12 (6) , 745-750. https://doi.org/10.1021/acsmacrolett.3c00183
- Riwang Li, Zhen Lin, Qian Zhang, Yuhui Zhang, Yi Liu, Yang Lyu, Xinyang Li, Changren Zhou, Gang Wu, Ningjian Ao, Lihua Li. Injectable and In Situ-Formable Thiolated Chitosan-Coated Liposomal Hydrogels as Curcumin Carriers for Prevention of In Vivo Breast Cancer Recurrence. ACS Applied Materials & Interfaces 2020, 12 (15) , 17936-17948. https://doi.org/10.1021/acsami.9b21528
- Ana Torres-Martínez, César A. Angulo-Pachón, Francisco Galindo, Juan F. Miravet. Liposome-Enveloped Molecular Nanogels. Langmuir 2019, 35 (41) , 13375-13381. https://doi.org/10.1021/acs.langmuir.9b02282
- Ilaria Clemente, Sara Falsini, Emanuela Di Cola, Giulia C. Fadda, Cristina Gonnelli, Francesco Spinozzi, Maria Bacia-Verloop, Isabelle Grillo, Sandra Ristori. Green Nanovectors for Phytodrug Delivery: In-Depth Structural and Morphological Characterization. ACS Sustainable Chemistry & Engineering 2019, 7 (15) , 12838-12846. https://doi.org/10.1021/acssuschemeng.9b01748
- Changwoo Nam, Jongsun Yoon, Sang A Ryu, Chang-Hyung Choi, Hyomin Lee. Water and Oil Insoluble PEGDA-Based Microcapsule: Biocompatible and Multicomponent Encapsulation. ACS Applied Materials & Interfaces 2018, 10 (47) , 40366-40371. https://doi.org/10.1021/acsami.8b16876
- Natkritta Hueppe, Frederik R. Wurm, Katharina Landfester. Nanocarriers with Multiple Cargo Load—A Comprehensive Preparation Guideline Using Orthogonal Strategies. Macromolecular Rapid Communications 2023, 44 (16) https://doi.org/10.1002/marc.202200611
- Soo Chin Yeoh, Choon Fu Goh. Topical delivery of salicylates. Drug Delivery and Translational Research 2022, 12 (5) , 981-1001. https://doi.org/10.1007/s13346-021-00988-5
- Riwang Li, Yang Lyu, Simin Luo, Huajun Wang, Xiaofei Zheng, Lihua Li, Ningjian Ao, Zhengang Zha. Fabrication of a multi-level drug release platform with liposomes, chitooligosaccharides, phospholipids and injectable chitosan hydrogel to enhance anti-tumor effectiveness. Carbohydrate Polymers 2021, 269 , 118322. https://doi.org/10.1016/j.carbpol.2021.118322