Contribution of Microorganisms with the Clade II Nitrous Oxide Reductase to Suppression of Surface Emissions of Nitrous OxideClick to copy article linkArticle link copied!
- Kristopher A. Hunt*Kristopher A. Hunt*Email: [email protected]. Phone: (206) 616-6985.Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195, United StatesMore by Kristopher A. Hunt
- Alex V. CarrAlex V. CarrDepartment of Molecular Engineering Sciences, University of Washington, Seattle, Washington 98105, United StatesInstitute for Systems Biology, Seattle, Washington 98109, United StatesMore by Alex V. Carr
- Anne E. OtwellAnne E. OtwellDepartment of Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195, United StatesMore by Anne E. Otwell
- Jacob J. ValenzuelaJacob J. ValenzuelaInstitute for Systems Biology, Seattle, Washington 98109, United StatesMore by Jacob J. Valenzuela
- Kathleen S. WalkerKathleen S. WalkerBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesDepartment of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United StatesMore by Kathleen S. Walker
- Emma R. DixonEmma R. DixonBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesDepartment of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United StatesMore by Emma R. Dixon
- Lauren M. LuiLauren M. LuiEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Lauren M. Lui
- Torben N. NielsenTorben N. NielsenEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Torben N. Nielsen
- Samuel BowmanSamuel BowmanDepartment of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02540, United StatesMore by Samuel Bowman
- Frederick von NetzerFrederick von NetzerDepartment of Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195, United StatesMore by Frederick von Netzer
- Ji-Won MoonJi-Won MoonBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesMore by Ji-Won Moon
- Christopher W. SchadtChristopher W. SchadtBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesMore by Christopher W. Schadt
- Miguel Rodriguez JrMiguel Rodriguez JrBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesMore by Miguel Rodriguez Jr
- Kenneth LoweKenneth LoweEnvironmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesMore by Kenneth Lowe
- Dominique JoynerDominique JoynerBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesDepartment of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United StatesMore by Dominique Joyner
- Katherine J. DavisKatherine J. DavisCenter for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United StatesMore by Katherine J. Davis
- Xiaoqin WuXiaoqin WuClimate and Ecosystem Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Xiaoqin Wu
- Romy ChakrabortyRomy ChakrabortyClimate and Ecosystem Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesMore by Romy Chakraborty
- Matthew W. FieldsMatthew W. FieldsCenter for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, United StatesDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United StatesMore by Matthew W. Fields
- Jizhong ZhouJizhong ZhouClimate and Ecosystem Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesInstitute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, United StatesState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, ChinaMore by Jizhong Zhou
- Terry C. HazenTerry C. HazenBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United StatesDepartment of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, United StatesMore by Terry C. Hazen
- Adam P. ArkinAdam P. ArkinEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United StatesDepartment of Bioengineering, University of California Berkeley, Berkeley, California 94720, United StatesMore by Adam P. Arkin
- Scott D. WankelScott D. WankelDepartment of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02540, United StatesMore by Scott D. Wankel
- Nitin S. BaligaNitin S. BaligaDepartment of Molecular Engineering Sciences, University of Washington, Seattle, Washington 98105, United StatesInstitute for Systems Biology, Seattle, Washington 98109, United StatesMore by Nitin S. Baliga
- David A. StahlDavid A. StahlDepartment of Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195, United StatesMore by David A. Stahl
Abstract
The sources and sinks of nitrous oxide, as control emissions to the atmosphere, are generally poorly constrained for most environmental systems. Initial depth-resolved analysis of nitrous oxide flux from observation wells and the proximal surface within a nitrate contaminated aquifer system revealed high subsurface production but little escape from the surface. To better understand the environmental controls of production and emission at this site, we used a combination of isotopic, geochemical, and molecular analyses to show that chemodenitrification and bacterial denitrification are major sources of nitrous oxide in this subsurface, where low DO, low pH, and high nitrate are correlated with significant nitrous oxide production. Depth-resolved metagenomes showed that consumption of nitrous oxide near the surface was correlated with an enrichment of Clade II nitrous oxide reducers, consistent with a growing appreciation of their importance in controlling release of nitrous oxide to the atmosphere. Our work also provides evidence for the reduction of nitrous oxide at a pH of 4, well below the generally accepted limit of pH 5.
Cited By
This article has not yet been cited by other publications.
Article Views
Altmetric
Citations
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.