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Metabolic Traits of Candidatus Accumulibacter clade IIF Strain SCELSE-1 Using Amino Acids As Carbon Sources for Enhanced Biological Phosphorus Removal

  • Guanglei Qiu*
    Guanglei Qiu
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
    *(G.Q.) E-mail: [email protected]
    More by Guanglei Qiu
  • Xianghui Liu
    Xianghui Liu
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    More by Xianghui Liu
  • Nay Min Min Thaw Saw
    Nay Min Min Thaw Saw
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
  • Yingyu Law
    Yingyu Law
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    More by Yingyu Law
  • Rogelio Zuniga-Montanez
    Rogelio Zuniga-Montanez
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    Department of Civil and Environmental Engineering, One Shields Avenue, University of California, Davis, California 95616, United States
  • Sara Swa Thi
    Sara Swa Thi
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    More by Sara Swa Thi
  • Thi Quynh Ngoc Nguyen
    Thi Quynh Ngoc Nguyen
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
  • Per H. Nielsen
    Per H. Nielsen
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    Centre for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, DK-9220, Aalborg, Denmark
  • Rohan B.H. Williams
    Rohan B.H. Williams
    Singapore Centre for Environmental Life Sciences Engineering, National University of Singapore, Singapore 119077, Singapore
  • , and 
  • Stefan Wuertz*
    Stefan Wuertz
    Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore 637551, Singapore
    Department of Civil and Environmental Engineering, One Shields Avenue, University of California, Davis, California 95616, United States
    School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798, Singapore
    *(S.W.) E-mail: [email protected]
Cite this: Environ. Sci. Technol. 2020, 54, 4, 2448–2458
Publication Date (Web):December 2, 2019
https://doi.org/10.1021/acs.est.9b02901
Copyright © 2019 American Chemical Society

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    Abstract

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    Despite recent evidence from full-scale plants suggesting that Candidatus Accumulibacter may be capable of using amino acids, this metabolic trait has never been confirmed in a bioreactor experiment. Here we show that an enriched culture of Ca. Accumulibacter clade IIF strain SCELSE-1 could metabolize 11 of 20 α-amino acids, with aspartate, glutamate, asparagine, and glutamine resulting in the highest phosphorus removal. The anaerobic uptake of aspartate and glutamate was achieved through a glutamate/aspartate-proton symporter fully powered by the proton motive force (PMF). Under anaerobic conditions aspartate was deaminized and routed into core carbon metabolic pathways to form polyhydroxyalkanoates (PHA). The lack of genes encoding NADH dependent isocitrate dehydrogenase in the Ca. Accumulibacter genome resulted in a kinetic barrier for glutamate to be channelled to the TCA cycle. Glutamate was stored as glutamate polymer. When amino acids (aspartate or glutamate) and acetate were supplied together, Ca. Accumulibacter took up both carbon sources simultaneously, with the uptake rate of each carbon source largely preserved. Overall energy savings (up to 17%) were achieved under mixed carbon scenarios, due to the ability of Ca. Accumulibacter to rearrange its anaerobic carbon metabolism based on the reducing power, PMF and ATP balance.

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    • Additional experimental details, results (Tables S1–S6 and Figures S1–S13), and discussion (PDF)

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