Exploring the Efficacy of Nile Red in Microplastic Quantification: A Costaining ApproachClick to copy article linkArticle link copied!
- Thomas Stanton*Thomas Stanton*E-mail: [email protected]School of Geography, University of Nottingham, NG7 2RD Nottingham, United KingdomFood, Water, Waste Research Group, Faculty of Engineering, University of Nottingham, NG7 2RD Nottingham, United KingdomMore by Thomas Stanton
- Matthew JohnsonMatthew JohnsonSchool of Geography, University of Nottingham, NG7 2RD Nottingham, United KingdomMore by Matthew Johnson
- Paul NathanailPaul NathanailLand Quality Management Ltd, University of Nottingham Innovation Park, NG7 2TU Nottingham, United KingdomMore by Paul Nathanail
- Rachel L. GomesRachel L. GomesFood, Water, Waste Research Group, Faculty of Engineering, University of Nottingham, NG7 2RD Nottingham, United KingdomMore by Rachel L. Gomes
- Teresa NeedhamTeresa NeedhamSchool of Geography, University of Nottingham, NG7 2RD Nottingham, United KingdomMore by Teresa Needham
- Amanda BursonAmanda BursonSchool of Geography, University of Nottingham, NG7 2RD Nottingham, United KingdomMore by Amanda Burson
Abstract
The presence of microplastic particles (<5 mm) in the environment has generated considerable concern across public, political, and scientific platforms. However, the diversity of microplastics that persist in the environment poses complex analytical challenges for our understanding of their prevalence. The use of the dye Nile red to quantify microplastics is increasingly common. However, its use in microplastic analysis rarely accounts for its affinity with the breadth of particles that occur in environmental samples. Here, we examine Nile red’s ability to stain a variety of microplastic particles and common natural and anthropogenic particles found in environmental samples. To better constrain microplastic estimates using Nile red, we test the coapplication of a second stain that binds to biological material, 4′,6-diamidino-2-phenylindole (DAPI). We test the potential inflation of microplastic estimates using Nile red alone by applying this costaining approach to samples of drinking water and freshwater. The use of Nile red dye alone resulted in a maximum 100% overestimation of microplastic particles. These findings are of particular significance for the public dissemination of findings from an emotive field of study.
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