Ternary Zn(II) Complexes of Fluorescent Zinc Probes Zinpyr-1 and Zinbo-5 with the Low Molecular Weight Component of Exchangeable Cellular Zinc PoolClick to copy article linkArticle link copied!
- Ilona MarszałekIlona MarszałekInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, PolandMore by Ilona Marszałek
- Wojciech GochWojciech GochInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, PolandFaculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-091 Warsaw, PolandMore by Wojciech Goch
- Wojciech Bal*Wojciech Bal*E-mail: [email protected]Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, PolandMore by Wojciech Bal
Abstract
The intracellular exchangeable Zn(II) is usually measured with synthetic fluorescent zinc sensors. 4′,5′-Bis[bis(2-pyridylmethyl)aminomethyl]-2′,7′-dichlorofluorescein (Zinpyr-1) is a sensor containing the fluorescein platform and a duplicated chelating unit. Its advantages include brightness and a relatively high affinity for Zn(II), Kd = 0.7 nM. 2-(4,5-Dimethoxy-2-hydroxyphenyl)-4-(2-pyridylmethyl)aminomethylbenzoxazole (Zinbo-5) is a member of a growing family of ratiometric synthetic Zn(II) probes, offering a possibility to determine Zn(II) concentration independently of the sensor concentration. Cells, however, contain high, millimolar or nearly millimolar concentrations of low molecular weight ligands (LMWLs) capable of binding Zn(II) ions. Previously, we demonstrated that such LMWLs can perturb the performance of some fluorescent zinc sensors by competition and formation of ternary Zn(sensor) (LMWL) complexes. Here we tested Zinpyr-1 and Zinbo-5 in this respect. Despite structural differences, both sensors formed such ternary complexes. We determined their stability constants CKtern and performed numerical simulations of Zn(II) distributions at physiological concentrations of selected LMWLs. Glutamic acid was found to provide the strongest ternary complexes with either of the studied sensors. Zn(Zinpyr-1)(Glu) was an absolutely dominant Zn(II)/Zinpyr-1 species (more than 96% of the exchangeable Zn(II)), and Zn(Zinbo-5)(Glu) was the most abundant one (more than 40%) in these simulations. Our results indicate that under cellular conditions these sensors are able to report Zn(II) complexed to LMWLs rather than free Zn2+ ions. On the other hand, the specific affinity of Zn(Zinpyr-1) and Zn(Zinbo-5) for Glu creates interesting opportunities for determining glutamic acid in biological samples.
Cited By
This article is cited by 11 publications.
- Marek Łuczkowski, Weronika Leszczyńska, Joanna Wątły, Stephan Clemens, Artur Krężel. Phytochelatins Bind Zn(II) with Micro- to Picomolar Affinities without the Formation of Binuclear Complexes, Exhibiting Zinc Buffering and Muffling Rather than Storing Functions. Inorganic Chemistry 2024, 63
(24)
, 10915-10931. https://doi.org/10.1021/acs.inorgchem.4c01707
- Claire M. S. Michielsen, Eva A. van Aalen, Maarten Merkx. Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn2+. ACS Chemical Biology 2022, 17
(6)
, 1567-1576. https://doi.org/10.1021/acschembio.2c00227
- Adarash Kumar Shukla, Ashutosh Mahale, Savita Choudhary, Pravesh Sharma, Onkar Prakash Kulkarni, Anupam Bhattacharya. Development and Validation of a Fluorogenic Probe for Lysosomal Zinc Release. ChemBioChem 2024, 25
(4)
https://doi.org/10.1002/cbic.202300783
- Alexey Krinochkin, Maria Valieva, Ekaterina Starnovskaya, Nataliya Slovesnova, Artem Minin, Anna Belousova, Leila Sadieva, Olga Taniya, Albert Khasanov, Alexander Novikov, Vitaly Bruskov, Svetlana Vatolina, Dmitry Kopchuk, Pavel Slepukhin, Vladimir Sharutin, Grigory Zyryanov. New Fluorescent Dye for the Detection of Zn2+ in Living Cells and Fixed Sections of the Rat Pancreas. Journal of Fluorescence 2024, 165 https://doi.org/10.1007/s10895-024-03603-1
- Sanjay Yadav, Nishu Choudhary, Alok Ranjan Paital. Tailored graphene/silica functional composite as signaling and adsorbent material: A sequential excimer probe with a fluoro-switching response with zinc and adenosine triphosphate. Carbon 2023, 205 , 527-539. https://doi.org/10.1016/j.carbon.2023.01.063
- Olivia R.M. Bagshaw, Ricardo Alva, Jeremy Goldman, Jaroslaw W. Drelich, Jeffrey A. Stuart. Mitochondrial zinc toxicity. 2023, 723-744. https://doi.org/10.1016/B978-0-323-88462-4.00008-0
- Wenlei Liu, Jana Jakobs, Lothar Rink. Proton-Pump Inhibitors Suppress T Cell Response by Shifting Intracellular Zinc Distribution. International Journal of Molecular Sciences 2023, 24
(2)
, 1191. https://doi.org/10.3390/ijms24021191
- Lucía López-Solís, Encarna Companys, Jaume Puy, Claudia A. Blindauer, Josep Galceran. Direct determination of free Zn concentration in samples of biological interest. Analytica Chimica Acta 2022, 1229 , 340195. https://doi.org/10.1016/j.aca.2022.340195
- Chen Zhang, Anna Dischler, Kaitlyn Glover, Yan Qin. Neuronal signalling of zinc: from detection and modulation to function. Open Biology 2022, 12
(9)
https://doi.org/10.1098/rsob.220188
- Lucía López-Solis, Josep Galceran, Jaume Puy, Encarna Companys. Absence of Gradients and Nernstian Equilibrium Stripping (AGNES): An Electroanalytical Technique for Chemical Speciation: A Tutorial Review. Chemosensors 2022, 10
(9)
, 351. https://doi.org/10.3390/chemosensors10090351
- Pritam Ghosh, Koushik Pramanik, Suparna Paul, Debanjan Dey, Swapan Kumar Chandra, Subhra Kanti Mukhopadhyay, Naresh Chandra Murmu, Priyabrata Banerjee. Zn
2+
Recognition for Pathogenesis of
Pick's Disease
via a Luminescent Test Kit. ChemistrySelect 2021, 6
(26)
, 6733-6739. https://doi.org/10.1002/slct.202100908
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.