Integration of Cryo-EM Model Building Software in Scipion
- M. Martínez
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- A. Jiménez-Moreno
- ,
- D. Maluenda
- ,
- E. Ramírez-Aportela
- ,
- R. Melero
- ,
- A. Cuervo
- ,
- P. Conesa
- ,
- L. del Caño
- ,
- Y. C. Fonseca
- ,
- R. Sánchez-García
- ,
- D. StrelakD. StrelakCNB-CSIC, C/Darwin 3, 28049 Madrid, SpainInstitute of Computer Science, Masaryk University, Botanická 68a, 60200 Brno, Czech RepublicMore by D. Strelak
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- J. J. Conesa
- ,
- E. Fernández-Giménez
- ,
- F. de Isidro
- ,
- C. O. S. Sorzano
- ,
- J. M. Carazo
- , and
- R. Marabini*R. Marabini*E-mail: [email protected]Escuela Politécnica, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 11, 28049 Madrid, SpainMore by R. Marabini
Abstract

Advances in cryo-electron microscopy (cryo-EM) have made it possible to obtain structures of large biological macromolecules at near-atomic resolution. This “resolution revolution” has encouraged the use and development of modeling tools able to produce high-quality atomic models from cryo-EM density maps. Unfortunately, many practical problems appear when combining different packages in the same processing workflow, which make difficult the use of these tools by non-experts and, therefore, reduce their utility. We present here a major extension of the image processing framework Scipion that provides inter-package integration in the model building area and full tracking of the complete workflow, from image processing to structure validation.
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