Pressure, Peptides, and a Piezolyte: Structural Analysis of the Effects of Pressure and Trimethylamine-N-oxide on the Peptide Solvation ShellClick to copy article linkArticle link copied!
- Angelina FolberthAngelina FolberthEduard-Zintl-Institut fuer Anorganische und Physikalische Chemie, Technical University of Darmstadt, Alarich-Weiss-Strasse 10, 64287 Darmstadt, GermanyMore by Angelina Folberth
- Jakub PolákJakub PolákPhysical Chemistry Department, University of Chemistry and Technology, Prague Technicka 5, 16628 Prague 6, Czech RepublicMore by Jakub Polák
- Jan Heyda*Jan Heyda*Email: [email protected]Physical Chemistry Department, University of Chemistry and Technology, Prague Technicka 5, 16628 Prague 6, Czech RepublicMore by Jan Heyda
- Nico F. A. van der Vegt*Nico F. A. van der Vegt*Email: [email protected]Eduard-Zintl-Institut fuer Anorganische und Physikalische Chemie, Technical University of Darmstadt, Alarich-Weiss-Strasse 10, 64287 Darmstadt, GermanyMore by Nico F. A. van der Vegt
Abstract
The osmolyte trimethylamine-N-oxide (TMAO) is able to increase the thermodynamic stability of folded proteins, counteracting pressure denaturation. Herein, we report experimental solubility data on penta-alanine (pAla) in aqueous TMAO solutions (at pH = 7 and pH = 13) together with molecular simulation data for pAla, penta-serine (pSer), and an elastin-like peptide (ELP) sequence (VPGVG) under varying pH and pressure conditions. The effect of the peptide end groups on TMAO–peptide interactions is investigated by comparing the solvation of zwitterionic and negatively charged pentamers with the solvation of pentamers with charge-neutral C- and N-termini and linear, virtually infinite, peptide chains stretched across the periodic boundaries of the simulation cell. The experiments and simulations consistently show that TMAO is net-depleted from the pAla–water interface, but local accumulation of TMAO is observed just outside the first hydration shell of the peptide. While the same observations are also made in the simulations of the zwitterionic pentamers (Ala, Ser, and ELP) and virtually infinite peptide chains (Ala and ELP), weak preferential binding of TMAO is instead observed for pAla with neutral end groups at a 1 M TMAO concentration and for an ELP pentamer with capped neutral end groups at a 0.55 M TMAO concentration studied in previous work (Y.-T. Liao et al. Proc. Natl. Acad. Sci. USA, 2017, 114, 2479–2484). The above observations made at 1 bar ambient pressure remain qualitatively unchanged at 500 bar and 2 kbar. Local accumulation of TMAO correlates with a reduction in the total number of peptide–solvent hydrogen bonds, independent of the peptide’s primary sequence and the applied pressure. By weakening water hydrogen bonds with the protein backbone, TMAO indirectly contributes to stabilizing internal hydrogen bonds in proteins, thus providing a protein stabilization mechanism beyond net depletion.
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This article is cited by 8 publications.
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https://doi.org/10.1063/5.0088388
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