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Quaternary Structure Analysis of a Hemoglobin Core in Hemoglobin–Albumin Cluster

  • Yoshitsugu Morita
    Yoshitsugu Morita
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
  • Taiga Yamada
    Taiga Yamada
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
    More by Taiga Yamada
  • Moeka Kureishi
    Moeka Kureishi
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
  • Kiyohito Kihira
    Kiyohito Kihira
    JEM Utilization Center, Human Spaceflight Technology Directorate, Japan Aerospace Exploration Agency (JAXA), 2-1-1 Sengen, Tsukuba-shi, Ibaraki 305-8505, Japan
  • , and 
  • Teruyuki Komatsu*
    Teruyuki Komatsu
    Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
    *E-mail: [email protected]. Tel/Fax: +81-3-3817-1910.
Cite this: J. Phys. Chem. B 2018, 122, 50, 12031–12039
Publication Date (Web):November 16, 2018
https://doi.org/10.1021/acs.jpcb.8b10077
Copyright © 2018 American Chemical Society

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    Abstract

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    A core–shell ensemble of bovine hemoglobin (Hb) and human serum albumin (HSA) is an artificial O2 carrier as a red blood cell substitute. This protein particle is created by covalent wrapping of a carbonyl Hb with HSAs: HbR–HSA3 cluster, where HbR signifies the use of carbonyl Hb (relaxed (R) state conformation) as a starting material. The HbR–HSA3 cluster exhibits high O2 affinity and low cooperativity. Analysis of the quaternary structure of the central HbR in the cluster revealed that its high O2 affinity is attributed to the physically immobile HbR nucleus. Circular dichroism and UV–vis absorption spectroscopy showed that the structure of deoxy HbR core closely resembles the R-state. The crystal structure of Lys-modified carbonyl HbR was superimposed on that of carbonyl Hb. These results imply that chemical modifications of the surface Lys groups and Cys-93(β) of the carbonyl Hb with cross-linking agent interfered in the quaternary structure movement from the R-state to tense (T) state. As expected, coupling of deoxy Hb (T-state) with HSAs yielded HbT–HSA3 cluster having low O2 affinity. The mixing of HbR–HSA3 and HbT–HSA3 clusters conferred a tailor-made formulation of artificial O2 carrier with a desired O2 affinity (P50).

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jpcb.8b10077.

    • SEC chromatogram and IEF (Figure S1); UV–vis absorption spectral data (Table S1); time-course of absorbance decays after the laser flash photolysis (Figure S2); CD spectra of deoxy SMP-HbT (Figure S3); X-ray crystallography data collection and refinement statistics (Table S2) (PDF)

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    7. Taiga Yamada, Maho Katsumi, Yuka Yagisawa, Masato Ichihara, Teruyuki Komatsu. Catalase–albumin cluster incorporating protoporphyrin IX: an O 2 generating photosensitizer for enhanced photodynamic therapy. Materials Advances 2022, 3 (16) , 6451-6457. https://doi.org/10.1039/D2MA00242F
    8. Yupeng Wang, Jie Yu, Zhijian Luo, Qiankun Shi, Guanglong Liu, Fan Wu, Zhizhang Wang, Yubin Huang, Dongfang Zhou. Engineering Endogenous Tumor‐Associated Macrophage‐Targeted Biomimetic Nano‐RBC to Reprogram Tumor Immunosuppressive Microenvironment for Enhanced Chemo‐Immunotherapy. Advanced Materials 2021, 33 (39) https://doi.org/10.1002/adma.202103497
    9. Yoshitsugu Morita, Ryoya Takada, Asuka Saito, Teruyuki Komatsu. Genetically and chemically tuned haemoglobin–albumin trimers with superior O 2 transport efficiency. Chemical Communications 2021, 57 (72) , 9144-9147. https://doi.org/10.1039/D1CC03684J
    10. Taiga Yamada, Teruyuki Komatsu. Methemoglobin‐Albumin Cluster Incorporating Protoporphyrin IX: Dual Functional Protein Drug for Photodynamic Therapy. ChemBioChem 2021, 22 (15) , 2526-2529. https://doi.org/10.1002/cbic.202100213
    11. Yoshitsugu Morita, Asuka Saito, Jun Yamaguchi, Teruyuki Komatsu. Haemoglobin(βK120C)–albumin trimer as an artificial O 2 carrier with sufficient haemoglobin allostery. RSC Chemical Biology 2020, 1 (3) , 128-136. https://doi.org/10.1039/D0CB00056F
    12. Ryosuke Funaki, Hitomi Iwasaki, Tomonori Kashima, Teruyuki Komatsu. Lyophilized hemoglobin‐albumin cluster with disaccharides: Long‐term storable powder of artificial O 2 ‐carrier. Polymers for Advanced Technologies 2020, 31 (5) , 1122-1126. https://doi.org/10.1002/pat.4846
    13. Ryosuke Funaki, Wataru Okamoto, Chihiro Endo, Yoshitsugu Morita, Kiyohito Kihira, Teruyuki Komatsu. Genetically engineered haemoglobin wrapped covalently with human serum albumins as an artificial O 2 carrier. Journal of Materials Chemistry B 2020, 8 (6) , 1139-1145. https://doi.org/10.1039/C9TB02184A
    14. Yoshitsugu Morita, Keisuke Igarashi, Ryosuke Funaki, Teruyuki Komatsu. Hemoglobin(βC93A)–Albumin Cluster: Mutation of Cysteine‐β93 to Alanine Allows Moderate Reduction of O 2 Affinity by Inositol Hexaphosphate. ChemBioChem 2019, 20 (13) , 1684-1687. https://doi.org/10.1002/cbic.201900079

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