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Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCII

Cite this: J. Phys. Chem. Lett. 2021, 12, 12, 3176–3181
Publication Date (Web):March 23, 2021
https://doi.org/10.1021/acs.jpclett.1c00122
Copyright © 2021 American Chemical Society
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    Abstract

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    Femtosecond transient absorption spectroscopy following two-photon excitation (2PE) is used to determine the contributions of carotenoids and chlorophylls to the 2PE signals in the main plant light-harvesting complex (LHCII). For 2PE, excitation at 1210 and 1300 nm was used, being within the known 2PE profile of LHCII. At both excitation wavelengths, the transient absorption spectra exhibit a shape characteristic of excited chlorophylls with only a minor contribution from carotenoids. We compare the 2PE data measured for LHCII with those obtained from 2PE of a lutein/chlorophyll a mixture in acetone. We estimate that although the 2PE cross section of a single carotenoid in acetone is ∼1.7 times larger than that of a Chl a, due to the 1:3.5 carotenoid/Chl ratio in LHCII, only one-third of the absorbed 2PE photons excite carotenoids in LHCII in the 1200–1300 nm range.

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jpclett.1c00122.

    • Transient absorption data after 2PE at 1300 nm; direct comparison of 2PE data obtained after 1210 and 1300 nm excitation; global fitting results; comparison of 1PE and 2PE data; excitation intensity dependence of 2PE signals; and the coherent artifact signal around time zero (PDF)

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    Cited By

    This article is cited by 9 publications.

    1. Hai-Dan Yao, Dan-Hong Li, Rong-Yao Gao, Cuicui Zhou, Wenda Wang, Peng Wang, Jian-Ren Shen, Tingyun Kuang, Jian-Ping Zhang. A Possible Mechanism for Aggregation-Induced Chlorophyll Fluorescence Quenching in Light-Harvesting Complex II from the Marine Green Alga Bryopsis corticulans. The Journal of Physical Chemistry B 2022, 126 (46) , 9580-9590. https://doi.org/10.1021/acs.jpcb.2c05823
    2. Václav Šebelík, Christopher D. P. Duffy, Erika Keil, Tomáš Polívka, Jürgen Hauer. Understanding Carotenoid Dynamics via the Vibronic Energy Relaxation Approach. The Journal of Physical Chemistry B 2022, 126 (22) , 3985-3994. https://doi.org/10.1021/acs.jpcb.2c00996
    3. Gürkan Keşan, Emrah Özcan, Pavel Chábera, Tomáš Polívka, Marcel Fuciman. Time‐Resolved Spectroelectrochemical Dynamics of Carotenoid 8’‐apo‐β‐Carotenal. ChemPlusChem 2023, 88 (11) https://doi.org/10.1002/cplu.202300404
    4. Olivia C. Fiebig, Dvir Harris, Dihao Wang, Madeline P. Hoffmann, Gabriela S. Schlau-Cohen. Ultrafast Dynamics of Photosynthetic Light Harvesting: Strategies for Acclimation Across Organisms. Annual Review of Physical Chemistry 2023, 74 (1) , 493-520. https://doi.org/10.1146/annurev-physchem-083122-111318
    5. Donatas Zigmantas, Tomáš Polívka, Petter Persson, Villy Sundström. Ultrafast laser spectroscopy uncovers mechanisms of light energy conversion in photosynthesis and sustainable energy materials. Chemical Physics Reviews 2022, 3 (4) https://doi.org/10.1063/5.0092864
    6. Juan Zhou, Weidong Yuan, Bao Di, Guanghua Zhang, Jianxi Zhu, Pengyu Zhou, Tianran Ding, Ji Qian. Relationship among Electrical Signals, Chlorophyll Fluorescence, and Root Vitality of Strawberry Seedlings under Drought Stress. Agronomy 2022, 12 (6) , 1428. https://doi.org/10.3390/agronomy12061428
    7. Premashis Manna, Gabriela S. Schlau-Cohen. Photoprotective conformational dynamics of photosynthetic light-harvesting proteins. Biochimica et Biophysica Acta (BBA) - Bioenergetics 2022, 1863 (4) , 148543. https://doi.org/10.1016/j.bbabio.2022.148543
    8. Minjung Son, Stephanie M. Hart, Gabriela S. Schlau-Cohen. Investigating carotenoid photophysics in photosynthesis with 2D electronic spectroscopy. Trends in Chemistry 2021, 3 (9) , 733-746. https://doi.org/10.1016/j.trechm.2021.05.008
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