Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCIIClick to copy article linkArticle link copied!
- Václav ŠebelíkVáclav ŠebelíkDepartment of Physics, Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05 České Budějovice, Czech RepublicMore by Václav Šebelík
- Valentyna KuznetsovaValentyna KuznetsovaDepartment of Physics, Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05 České Budějovice, Czech RepublicMore by Valentyna Kuznetsova
- Heiko Lokstein*Heiko Lokstein*Email: [email protected]Department of Chemical Physics and Optics, Charles University, Ke Karlovu 3, 121 16 Prague 2, Czech RepublicMore by Heiko Lokstein
- Tomáš Polívka*Tomáš Polívka*Email: [email protected]Department of Physics, Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05 České Budějovice, Czech RepublicMore by Tomáš Polívka
Abstract
Femtosecond transient absorption spectroscopy following two-photon excitation (2PE) is used to determine the contributions of carotenoids and chlorophylls to the 2PE signals in the main plant light-harvesting complex (LHCII). For 2PE, excitation at 1210 and 1300 nm was used, being within the known 2PE profile of LHCII. At both excitation wavelengths, the transient absorption spectra exhibit a shape characteristic of excited chlorophylls with only a minor contribution from carotenoids. We compare the 2PE data measured for LHCII with those obtained from 2PE of a lutein/chlorophyll a mixture in acetone. We estimate that although the 2PE cross section of a single carotenoid in acetone is ∼1.7 times larger than that of a Chl a, due to the 1:3.5 carotenoid/Chl ratio in LHCII, only one-third of the absorbed 2PE photons excite carotenoids in LHCII in the 1200–1300 nm range.
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