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Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCII
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    Transient Absorption of Chlorophylls and Carotenoids after Two-Photon Excitation of LHCII
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    The Journal of Physical Chemistry Letters

    Cite this: J. Phys. Chem. Lett. 2021, 12, 12, 3176–3181
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    https://doi.org/10.1021/acs.jpclett.1c00122
    Published March 23, 2021
    Copyright © 2021 American Chemical Society

    Abstract

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    Femtosecond transient absorption spectroscopy following two-photon excitation (2PE) is used to determine the contributions of carotenoids and chlorophylls to the 2PE signals in the main plant light-harvesting complex (LHCII). For 2PE, excitation at 1210 and 1300 nm was used, being within the known 2PE profile of LHCII. At both excitation wavelengths, the transient absorption spectra exhibit a shape characteristic of excited chlorophylls with only a minor contribution from carotenoids. We compare the 2PE data measured for LHCII with those obtained from 2PE of a lutein/chlorophyll a mixture in acetone. We estimate that although the 2PE cross section of a single carotenoid in acetone is ∼1.7 times larger than that of a Chl a, due to the 1:3.5 carotenoid/Chl ratio in LHCII, only one-third of the absorbed 2PE photons excite carotenoids in LHCII in the 1200–1300 nm range.

    Copyright © 2021 American Chemical Society

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jpclett.1c00122.

    • Transient absorption data after 2PE at 1300 nm; direct comparison of 2PE data obtained after 1210 and 1300 nm excitation; global fitting results; comparison of 1PE and 2PE data; excitation intensity dependence of 2PE signals; and the coherent artifact signal around time zero (PDF)

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    Cited By

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    This article is cited by 14 publications.

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    The Journal of Physical Chemistry Letters

    Cite this: J. Phys. Chem. Lett. 2021, 12, 12, 3176–3181
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jpclett.1c00122
    Published March 23, 2021
    Copyright © 2021 American Chemical Society

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